Amphoteric glycolipid biosurfactant and preparation method thereof

A bio-surface, amphoteric glycolipid technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as the decline of the overall effect, and achieve the effects of low cost, excellent surface and interface activity, and simple preparation method.

Active Publication Date: 2021-01-01
夏文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Then once different types of surfactants are introduced, under certain conditions, the overall effect tends to be greatly reduced

Method used

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  • Amphoteric glycolipid biosurfactant and preparation method thereof
  • Amphoteric glycolipid biosurfactant and preparation method thereof
  • Amphoteric glycolipid biosurfactant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation method of amphoteric glycolipid biosurfactant molecule is

[0058] 1. Bacteria: Amphoteric glycolipid biosurfactants need to be cultivated in sequence with three bacteria, Pseudomonas aeruginosa (ATCC 15442), Candida albicans (ATCC 10231), and Neurospora crassa (ATCC 18889).

[0059] 2. Medium: NaNO 3 : 0.4%, FeCl 2 : 0.002%, NaH 2 PO 4 : 0.25%, K 2 HPO 4 : 0.25%, MgSO 4 ·7H 2 O: 0.005%, KCl: 0.05%, Choline chloride: 0.05%, fatty amine: 0.5%, corn syrup: 0.05%, soybean oil 5%: 3.0%, glucose: 0.1%, yeast extract powder: 0.001%, initial pH 6 -7.

[0060] 3. Seed shake flask culture

[0061] The slant strains were cultured for 48 hours, and the three above-mentioned single-bacteria slants were respectively transferred to 1L Erlenmeyer flasks, in which 200mL of LB or YPD medium was filled in the Erlenmeyer flasks, and Pseudomonas and Candida were cultured at about 35°C. Candida was cultured at about 25°C, and Neurospora was cultured at about 30°C f...

Embodiment 2

[0085] Preparation method of amphoteric glycolipid biosurfactant molecule

[0086] 1. Bacteria: Amphoteric glycolipid biosurfactants need to be cultivated in sequence with three bacteria, Pseudomonas aeruginosa (ATCC 15442), Candida albicans (ATCC 10231), and Neurospora crassa (ATCC 18889).

[0087] 2. Medium: NaNO 3 : 1.4%, FeCl 2 : 0.005%, NaH 2 PO 4: 0.4%, K 2 HPO 4 : 0.4%, MgSO 4 ·7H 2 O: 0.01%, KCl: 0.09%, Choline chloride: 0.05%, fatty amine: 0.5%, corn syrup: 0.1%, soybean oil: 4.0%, glucose: 0.3%; yeast infusion powder: 0.004%, initial pH 6-7 .

[0088] 3. Seed shake flask culture

[0089] The slant strains were cultured for 36h-48h, and the three above-mentioned single-bacteria slants were transferred to 1L conical flasks respectively. The conical flasks were filled with 200mL of LB or YPD medium, and Pseudomonas was cultured at about 35°C. Candida candida was cultured at about 25°C, and Neurospora was cultured at about 30°C for 30 hours.

[0090] 4. Produc...

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Abstract

The invention discloses an amphoteric glycolipid biosurfactant and a preparation method thereof, and belongs to the technical field of biological materials. The amphoteric glycolipid biosurfactant comprises fatty acid, polysaccharide, amphoteric glycolipid biosurfactant molecules, glycosamine, lipopeptide, ethanol, fatty amine and the like. The preparation method of the amphoteric glycolipid biosurfactant comprises the following steps: (1) culturing: carrying out staged culture by adopting three bacteria; (2) separating: adjusting the pH value of the obtained fermentation culture solution, centrifuging, and recovering a middle-layer clear solution; (3) performing acid precipitation: adjusting the pH value of the middle-layer clear solution, and then performing refrigerating precipitation;(4) filtering: collecting the precipitate by adopting a ceramic membrane, then dissolving the precipitate, and filtering again to obtain an aqueous solution; (5) concentrating: concentrating the aqueous solution in vacuum to obtain a concentrated aqueous solution; and (6) obtaining the finished product: adding the above components into the concentrated aqueous solution, and stirring. The preparedamphoteric glycolipid biosurfactant is a high-quality amphoteric glycolipid biosurfactant.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to an amphoteric glycolipid biosurfactant and a preparation method thereof. Background technique [0002] Biosurfactants refer to active substances with surface activity formed by microorganisms through their own metabolism. These substances have special hydrophilic groups and hydrophobic groups, and have a more complex structure than chemical surfactants. Therefore, it has a wide range of uses and has been widely used in industries such as chemical industry, medicine, cosmetics, oil recovery and environmental pollution control. According to the structural characteristics of biosurfactants, they can be divided into polymers, lipopeptides, neutral lipids, phospholipids, etc. Currently, biosurfactant-producing bacteria include Enterococcus, Pseudomonas, Streptomyces, Bacillus licheniformis, Acinetobacter, and yeast. Under certain culture conditions, such as suitable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/00C12P1/04C12P1/02C12R1/38C12R1/72C12R1/01C09K23/00
CPCC12P1/04C12P1/02C12R2001/01C12N1/205C12R2001/38C12R2001/72C12N1/165C09K23/00C09K23/017
Inventor 夏文杰
Owner 夏文杰
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