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A biomimetic targeting of TIO with a PD-L1-loaded antibody 2 Nanoparticles and their preparation and use

A PD-L1 and nanoparticle technology, applied in the direction of antibodies, antibody medical components, anti-tumor drugs, etc., can solve the problems of insufficient targeting and efficacy, achieve good homologous targeting and specificity, and inhibit malignant melanoma. The effect of growth, strong target aggregation ability

Active Publication Date: 2022-03-18
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the prior art modified TiO 2 The application of nanometer in SDT has the problem of insufficient targeting and curative effect

Method used

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  • A biomimetic targeting of TIO with a PD-L1-loaded antibody <sub>2</sub> Nanoparticles and their preparation and use
  • A biomimetic targeting of TIO with a PD-L1-loaded antibody <sub>2</sub> Nanoparticles and their preparation and use
  • A biomimetic targeting of TIO with a PD-L1-loaded antibody <sub>2</sub> Nanoparticles and their preparation and use

Examples

Experimental program
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Embodiment 1

[0134] Example 1. Biomimetic targeting of TiO loaded with PD-L1 antibody of the present invention 2 Preparation of nanoparticles

[0135] 1. Membrane extraction of mouse malignant melanoma B16F10 cells

[0136] (1) Collect B16F10 cells (approximately 1×10 8 cells);

[0137] (2) Centrifuge at 1000g for 5min, wash twice with PBS; resuspend cells with 1mL buffer. The pH of the buffer solution is 7.2, with 10mM tris(hydroxymethyl)aminomethane (Tris(hydroxymethyl)aminomethane, Tris) as solvent, add MgCl 2 (100mM) 0.1mL and 100× EDTA-free protease inhibitor (10μL);

[0138] (3) Use an ultrasonic cell disruptor (power 300W, frequency 21KHz, 50% duty cycle) to disrupt the cells for 30 minutes in an ice bath; collect the supernatant after centrifugation (4000g) at 4°C for 10 minutes;

[0139] (4) The supernatant was then centrifuged at 4°C for 30 minutes at low temperature (16000g), and washed twice with PBS after discarding the supernatant;

[0140] (5) Ultrasonic resuspension i...

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Abstract

The invention provides a biomimetic targeting TiO loaded with PD‑L1 antibody 2 The nanoparticle and its preparation method and application belong to the field of biomedical materials. The TiO 2 Nanoparticles are biomimetic TiO loaded with programmed cell death receptor-ligand 1 antibody 2 nanoparticles; the biomimetic TiO 2 Nanoparticles made of TiO 2 Nanoparticles, DSPE‑PEG2000‑NH 2 and cell membranes as raw materials; TiO 2 Nanoparticles, DSPE‑PEG2000‑NH 2 The mass ratio to the cell membrane is (1-5):(1-5):(1-5). The nanoparticle has a small particle size, a high antibody carrying rate, and no obvious cytotoxicity. At the same time, the nanoparticle can better homologously target and specifically target and bind malignant melanoma cells, thereby causing cell apoptosis. In addition, the nanometer is biologically safe, has a relatively strong targeting aggregation ability after intratumoral injection, and can better inhibit the growth of malignant melanoma under SDT conditions. It can be used for the treatment of tumors, especially malignant melanoma, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a biomimetic targeting TiO loaded with PD-L1 antibody 2 Nanoparticles and methods for their preparation and use. Background technique [0002] Malignant tumors are one of the most serious diseases that threaten human health and life worldwide. At present, early malignant tumors can be treated by traditional treatment methods such as surgery, chemotherapy, radiotherapy, etc., but these methods have little effect on patients with advanced tumors, and huge toxic side effects such as liver and kidney damage may accelerate the progression of the disease. deterioration and death. Therefore, there is an urgent need for some new therapeutic methods for adjuvant tumor therapy. With the continuous development of medical technology, some emerging therapies such as biological therapy, immunotherapy, photodynamic and sonodynamic therapy have attracted more and more attention. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K47/02A61K39/395A61K41/00A61P35/00
CPCA61K9/143A61K39/3955A61K41/0033A61P35/00A61K2300/00
Inventor 邱逦马朗韦馨程冲向茜王丽芸朱笔挥李玲
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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