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Fructus aurantii quality marker and screening method and application thereof

A marker, the technology of Citrus aurantium, applied in the field of medicine, can solve the problems of uneven quality, variety and confusion of Citrus aurantii, and achieve the effect of solving unclear expectations

Inactive Publication Date: 2020-12-22
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, the Fructus Fructus Fructus produced in Jiangxi is used as the authentic medicinal material, which is of good quality and has exact medicinal effects.

Method used

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  • Fructus aurantii quality marker and screening method and application thereof
  • Fructus aurantii quality marker and screening method and application thereof
  • Fructus aurantii quality marker and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052]The chemical composition and main chemical components of Fructus Aurantii were obtained using molecular network research methods.

[0053]Solid phase extraction and enrichment method: 1) Solid phase extraction cartridge activation: The solid phase extraction cartridge uses pure acetonitrile 1mL each time, rinse three times, and then pure water 1mL each time, rinse three times. 2) Sample loading: After spin-drying the 50% alcohol extract of Fructus Aurantii from Gan, reconstituted with 1 mL of 30% acetonitrile, centrifuged at 18000g, and the supernatant was slowly loaded. 3) Elution: Wash with 30% acetonitrile, 50% acetonitrile, 70% acetonitrile, and 90% acetonitrile respectively after loading, and wash each gradient with 1 mL each time, three times. 4) Concentration: After rotary evaporation of each gradient fraction, 1 mL 70% acetonitrile is added for reconstitution, centrifuged, the supernatant is taken for injection, and UPLC-MS acquisition chromatography and corresponding pri...

Embodiment 2

[0057]Metabonomics was used to distinguish the chemical components of Fructus Aurantii produced in Jiangxi and Fructus Aurantii produced in non-Gan.

[0058]Collection of medicinal materials: 12 batches of Citrus aurantium medicinal materials were collected in Jiangxi Province in 2017, numbered ZQ1-12; 3 batches of medicinal materials were collected in Yiyang City, Hunan Province, numbered ZQ13-15; 5 batches of medicinal materials were collected in Chongqing City, numbered ZQ16- twenty one. All specimens were identified by Professor Zhang Shouwen of Jiangxi University of Traditional Chinese Medicine and stored in a refrigerator at 4°C in our laboratory.

[0059]Extraction of medicinal materials: After crushing the medicinal materials of Citrus aurantium, sieving the fine powder of medicinal materials through a 40-mesh standard sieve. Accurately weigh 50g powder and add 10 times the amount of 50% ethanol for reflux extraction three times. Filter, combine the three filtrates, concentrate un...

Embodiment 3

[0062]The serum medicinal chemistry study of Fructus Aurantii screened the blood components of Fructus Aurantii.

[0063]Clean SD rats (male, 20-22g) were purchased from the Experimental Animal Center of Kunming Medical University. Ten SD rats were randomly divided into two groups. The Fructus Aurantii group was given 1g / kg Fructus Aurantii freeze-dried powder suspension by intragastric administration, and the blank group was given distilled water treatment with a gastric volume of 2mL / 200g. The treatment was given for three consecutive days. All rats were given blood from the vein at 1h, 2h, 3h, 4h, 6h and 24h after the administration, and the serum was collected by centrifugation at 2000g / 4°C. After the liquid phase detection, non-target drug metabolomics was used to screen the blood components of Gardenia.

[0064]The experimental results showed that the chemical species of Fructus Aurantii were exposed to more than 1 hour after the administration of Fructus Aurantii. Under physiologic...

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Abstract

The invention discloses a fructus aurantii quality marker and a screening method thereof. The method includes the steps that firstly, chemical phenotypes and difference components of Jiangxi genuine fructus aurantii and non-genuine fructus aurantii are compared, and finally, a chemical component group with the stomach invigorating effect in fructus aurantii is determined through pharmacological research on serum medicinal chemistry and in-vitro, in-vivo and virtual stomach invigorating activity screening. It is found that intragastric administration through the fructus aurantii extract can remarkably resist atropine-induced gastrointestinal motility disorder, the chemical component group capable of reflecting clinical medication effectiveness of fructus aurantii is finally determined through screening of the stomach invigorating activity of in-vitro chemical components, and the chemical component group can serve as a quality marker of fructus aurantii, and the quality control of the fructus aurantii based on the medicinal components is realized. According to the invention, the traditional Chinese medicine quality control problems that the quality of fructus aurantii medicinal materials is uneven, the clinical efficacy is unstable, the traditional fructus aurantii quality standard does not reflect the unrelated efficacy and the like are solved.

Description

Technical field:[0001]The invention belongs to the technical field of medicine, and relates to the discovery and application of quality markers of traditional Chinese medicine. Specifically, it relates to an active ingredient group representing the medicinal effect of Fructus Aurantii, a quality marker of Fructus Aurantii and a screening method thereof.Background technique:[0002]Traditional Chinese medicine is a complex and rich chemical entity library. Traditional Chinese medicine quality control only uses simple fingerprints and quantitative determination of single components to evaluate its quality. There is limited quality control and cannot fully and objectively reflect the complexity and complexity of chemical components of Chinese medicine. Diversity. In addition, traditional indicators have poor specificity and cannot be associated with drug effects.[0003]As a current emerging quality control idea of ​​traditional Chinese medicine, the quality-marker of traditional Chinese m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/86
CPCG01N30/02G01N30/72G01N30/8686G01N30/8696
Inventor 李飞王义坤
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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