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A kind of method and application of using chrome leather shavings to prepare white rot fungus solid medium

A technology of solid medium and white rot fungi, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of reducing the chromium content of hydrolyzed protein, achieve smooth reaction, good cultivation effect, and small fluctuations Effect

Active Publication Date: 2022-06-21
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the current dechroming method is difficult to completely remove chromium, it is an effective means to reduce the chromium content in hydrolyzed protein and control it in an appropriate range through certain methods, and then apply to the fermentation culture of specific microorganisms

Method used

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  • A kind of method and application of using chrome leather shavings to prepare white rot fungus solid medium
  • A kind of method and application of using chrome leather shavings to prepare white rot fungus solid medium
  • A kind of method and application of using chrome leather shavings to prepare white rot fungus solid medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing white rot fungus solid culture medium utilizing chrome leather scraps, comprising the following steps:

[0038] (1) Hydrolysis and dechromization: in a constant temperature water bath at 60°C, add 25g of chrome leather scraps (containing Cr to the Erlenmeyer flask) 2 O 3: 3.2%), then add 3g of calcium oxide and 300g of deionized water, after stirring and reacting for 0.5h, add 12.5g of chrome leather scraps, continue to react for 0.5h, then add 12.5g of chrome leather scraps and continue to react for 1h to obtain a thick The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 mi...

Embodiment 2

[0052] A method for preparing white rot fungus solid culture medium utilizing chrome leather scraps, comprising the following steps:

[0053] (1) Hydrolytic dechromization: in a constant temperature water bath at 60 °C, add 30 g of chrome leather scraps (containing Cr 2 O 3 : 2.5%), then add 4.2g of calcium oxide and 390g of deionized water, after stirring and reacting for 0.5h, add 15g discarded chrome leather scraps, continue the reaction for 0.5h, then add 15g chrome leather scraps and continue the reaction for 1h to obtain a thick The homogeneous mixture of hydrolyzed protein was removed by centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 min, discard the p...

Embodiment 3

[0067] A method for preparing white rot fungus solid culture medium utilizing chrome leather scraps, comprising the following steps:

[0068] (1) Hydrolytic dechromization: in a constant temperature water bath at 80°C, add 20g of chrome leather scraps (containing Cr 2 O 3 : 5%), then add 3.6g of calcium oxide, 400g of deionized water, after stirring for 0.5h, add 10g of chrome leather scraps, continue to react for 0.5h, then add 10g of chrome leather scraps and continue to react for 1h to obtain a viscous The hydrolyzed protein homogenate was subjected to centrifugal suction filtration while hot to remove insoluble solids, the filtrate was collected, the hydrolyzed protein sample obtained after dechromization was treated with carbon dioxide, the white precipitated calcium carbonate was removed by suction filtration, the filtrate was collected, and the filtrate was placed in a centrifuge. Centrifuge at 8000 r / min for 5 min, discard the precipitate, collect the liquid, measure ...

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Abstract

The invention belongs to the field of solid leather wastes, and in particular relates to a method and application for preparing a solid medium for white-rot fungi by using chrome leather shavings. Firstly, the waste chrome leather shavings from tanning were used as raw materials, and the dechromed hydrolyzed collagen was prepared by batch-feeding process based on the alkali-enzyme hydrolysis system. Secondly, using the extracted dechromed hydrolyzed collagen as a nutrient source, compound glucose, a small amount of peptone, trace element solution, agar, vitamin B1, and purified water to prepare a solid fermentation medium; finally, use the solid medium to ferment and cultivate white rot fungi. The prepared solid medium can realize the normal and efficient growth of white-rot fungi, not only realize the purpose of turning chromium leather scraps into treasure, but also greatly replace traditional peptone resources, effectively reduce the cultivation cost of white-rot fungi, and benefit High-value utilization of solid waste containing chromium protein.

Description

technical field [0001] The invention belongs to the field of leather solid waste, and in particular relates to a method and application for preparing a solid culture medium for white rot fungi by utilizing chrome leather scraps. Background technique [0002] Since the 1990s, the leather industry has developed rapidly, and China has gradually become the world's largest tannery country, but the accompanying pollution problem has also become increasingly serious. Especially in the tanning production process, 70% of the waste is chromium-containing waste, which mainly includes chromium leather scraps, chromium-containing leather blocks, and leather dust. Nitrogen sources, on the other hand, are nutrients that constitute sources of nitrogen in microbial cells and metabolites. Tannery waste is rich in collagen and its hydrolyzed products (polypeptides, amino acids, etc.) to different degrees, which can provide the necessary organic nitrogen source for the growth and reproduction ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P21/06C12R1/645
CPCC12N1/14C12P21/06
Inventor 刘洁张斐斐徐洲李彦春李雪松周玉
Owner QILU UNIV OF TECH
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