Seawater spirulina-phycomycetes symbiotic system, construction method and application
A symbiotic system and construction method technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high sludge treatment cost, high cost, high cost, etc., and achieve good denitrification and phosphorus removal effect. , Solve the effect of adding carbon source and promoting growth rate
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Embodiment 1
[0060] In the present invention, seawater spirulina and Nitratireductor sp.strain ZYF759 are mixed and cultivated to obtain a stable algae-bacteria symbiosis system, and the inoculated biomass ratio of seawater spirulina and Nitratireductor sp.strain ZYF759 is 3:1;
[0061] Described seawater spirulina medium is seawater Zarrouk medium;
[0062] Described Nitratireductor sp.strain ZYF759 medium is 2216E liquid medium;
[0063] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL seawater Zarrouk medium is housed in the 1000mL Erlenmeyer flask;
[0064] The inoculation method is to centrifuge the seawater Spirulina culture medium and the Nitratireductor sp.strain ZYF759 culture medium separately, centrifuge at 5000 rpm for 5 minutes, discard the supernatant, resuspend and mix with fresh Zarrouk medium;
[0065] The temperature of the mixed culture was 30°C, and the light intensity was 60 μmol m -1 ·s -1 , ...
Embodiment 2
[0071] In the present invention, seawater spirulina and Nitratireductor sp.strain ZYF759 are mixed and cultivated to obtain a stable algae-bacteria symbiosis system, and the inoculated biomass ratio of seawater spirulina and Nitratireductor sp.strain ZYF759 is 3:1;
[0072] Described seawater spirulina medium is seawater Zarrouk medium;
[0073] Described Nitratireductor sp.strain ZYF759 medium is 2216E liquid medium;
[0074] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL of simulated seawater aquaculture tail water is housed in the 1000mL Erlenmeyer flask;
[0075] The composition of the simulated mariculture tail water is: 132mg L -1 Glucose, 33.71mg·L -1 Ammonium chloride, 25.25mg·L -1 Potassium nitrate, 15.55mg·L -1 Potassium dihydrogen phosphate, 90mg·L -1 Magnesium sulfate, 14mg·L -1 Calcium chloride, 0.25mg·L -1 Cobalt chloride hexahydrate, 0.5mg L -1 Copper sulfate pentahydrate, 0.5mg ...
Embodiment 3
[0085]In the present invention, seawater spirulina and Microcellaalkaliphila, strain:JAM AC0309 are mixed and cultured to obtain a stable algae-bacteria symbiosis system, and the inoculated biomass ratio of seawater spirulina and Microcellaalkaliphila,strain:JAM AC0309 is 3:1;
[0086] Described seawater spirulina medium is seawater Zarrouk medium;
[0087] Described Microcellaalkaliphila, strain: JAM AC0309 medium is 2216E liquid medium;
[0088] The mixed culture container is a 1000mL Erlenmeyer flask, and three parallel experiments are set for each group, and 500mL seawater Zarrouk medium is housed in the 1000mL Erlenmeyer flask;
[0089] The inoculation method is to centrifuge the seawater Spirulina culture medium and Microcellaalkaliphila, strain:JAM AC0309 culture medium separately, centrifuge at 5000rpm for 5min, discard the supernatant, and use fresh Zarrouk medium to resuspend and inoculate;
[0090] The temperature of the mixed culture was 30°C, and the light intens...
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