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Single-cell Raman clinical drug resistance kit and detection method

A technology of Raman drug resistance and kits, which is applied in Raman scattering, biochemical equipment and methods, and measurement/testing of microorganisms, can solve the problems of slow detection of clinical drug-resistant pathogens, and achieve high accuracy, Simple operation and high sensitivity effect

Pending Publication Date: 2020-12-15
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a single-cell Raman clinical drug resistance rapid detection kit and detection method that can solve the problem of slow detection of clinical drug-resistant pathogenic bacteria in the current technology

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  • Single-cell Raman clinical drug resistance kit and detection method

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Embodiment 1

[0031] Embodiment 1 kit composition information

[0032] The information of the kit in this embodiment is shown in Table 1. The incubation solution is 100% heavy water, the fixative solution I is 0.9% normal saline, the fixative solution II is absolute ethanol, and the ultrapure water and culture medium can be prepared by yourself. .

[0033] Table 1. Kit composition information

[0034]

[0035]

[0036] Table 2 shows the formula of the incubation system prepared when using the above-mentioned kits for rapid detection of single-cell Raman clinical drug resistance. The volume of the test drug is adjusted according to different samples.

[0037] Table 2. Preparation of incubation system

[0038] components volume 2×Medium (2×Medium) 5.0mL Incubation Buffer 3.0mL Clinical samples to be tested 1.0mL Test Drugs (Antibiotics) n mL ultrapure waterddH 2 o

Replenish to 10.0mL Total 10.0mL

[0039] Table 3 shows the...

Embodiment 2

[0042] Example 2 Detection of drug resistance of Escherichia coli (E.coli395) in clinical urine samples by single-cell Raman clinical drug resistance quick test kit

[0043] In this example, the single-cell Raman clinical drug resistance quick detection kit is used to detect the drug resistance of Escherichia coli (E.coli395) in clinical urine samples of patients with urinary tract infection, and the corresponding test drug (antibiotic) is Levofloxacin (Levofloxacin, Levo), the specific drug resistance detection steps are as follows:

[0044] 1. Clinical urine sample and drug pretreatment

[0045] Collect 1.0mL clinical fresh urine sample, centrifuge (8000rpm, 2min), remove the supernatant, add 1.0mL PBS to resuspend for use.

[0046] 2. Preparation of incubation system

[0047] Carry out the establishment of the incubation system of control group and experimental group according to Table 4, do not add one group of levofloxacin (Levo) as control group (with ultrapure water d...

Embodiment 3

[0062] Example 3 Detection of drug resistance of Escherichia coli (E.coli865) in clinical urine samples by single-cell Raman clinical drug resistance quick test kit

[0063] In this example, the single-cell Raman clinical drug resistance quick detection kit is used to detect the drug resistance of Escherichia coli (E.coli865) in clinical urine samples of patients with urinary tract infection, and the corresponding test drug (antibiotic) is Gentamicin Gentamicin (Gentamicin, Genta), the specific drug resistance detection steps are as follows:

[0064] 1. Clinical urine sample and drug pretreatment

[0065] Collect 1.0mL clinical fresh urine sample, centrifuge (8000rpm, 2min), remove the supernatant, add 1.0mL PBS to resuspend for use.

[0066] 2. Preparation of incubation system

[0067] According to Table 5, the establishment of the incubation system of the control group and the experimental group was carried out, and the group without gentamicin (Genta) was used as the cont...

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Abstract

The invention discloses a single-cell Raman clinical drug resistance rapid detection kit and a detection method. The single-cell Raman clinical drug resistance rapid detection kit comprises an incubation solution, a stationary solution I and a stationary solution II, wherein the incubation solution contains heavy water; the stationary solution I contains normal saline or PBS; and the stationary solution II contains absolute ethyl alcohol. The kit is used for stable isotope labeled single-cell Raman drug resistance detection, can realize rapid detection of drug resistance phenotypes of trace microorganisms in a clinical sample without dependence on proliferation, and can solve the problem of slow detection of clinical drug-resistant pathogenic bacteria in the prior art.

Description

technical field [0001] The invention relates to the technical field of microbial drug resistance detection, in particular to a single-cell Raman rapid detection kit for clinical drug resistance and its detection method and application. Background technique [0002] Antibiotics are an indispensable clinical drug for the treatment of pathogenic bacterial infections, but in recent years, the therapeutic effect of antibiotics on pathogenic bacteria has become worse and worse. The abuse of antibiotics has led to the widespread spread of drug resistance, and microbial resistance is still A conundrum in the medical world. After the human body is infected by pathogenic bacteria, if the doctor cannot use antibiotics correctly and targetedly for treatment, it will not only consume time and effort and waste treatment costs, but may also kill the probiotics in the body, causing some conditional pathogenic bacteria to produce disease. Sex, more likely to form "super bacteria". "Superbu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18G01N21/65
CPCC12Q1/18G01N21/65
Inventor 徐健朱鹏飞戴靖籍月彤马波
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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