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Hypsizygus marmoreus strain

A technology of shiitake mushrooms and strains, which is applied in the directions of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems that the hollow stipe is not strong enough, and is prone to the occurrence of nodular mushrooms, and achieves the effect of good promotion prospects.

Active Publication Date: 2020-12-11
FUJIAN AGRI & FORESTRY UNIV +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a No. 1 jitake mushroom strain, Fuhong No. 1, which solves the technical problems that stropharia, stipe fluff, and thick stipe are hollow and not strong enough in the existing production of jijitake mushroom strains.

Method used

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  • Hypsizygus marmoreus strain
  • Hypsizygus marmoreus strain

Examples

Experimental program
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Effect test

Embodiment 1

[0024] True Ji mushroom ‘Hy0051’ is a strain obtained from ‘Shennong Baixue’ seafood mushroom purchased in the market by the method of fruiting body tissue separation. (References for fruiting body tissue separation method: Wang Xiuyan, Preparation of edible mushroom parent species by fruiting body tissue separation method, Journal of Chifeng University (Natural Science Edition), 2016, 32(17): 13-14. Ke Lina, Lai Zhibin, etc., Pleurotus eryngii Factory cultivation test of strains isolated from tissue, Jiangsu Agricultural Science, 2015, 43(8):239-241)

[0025] True Jiji mushroom 'Hy0056' is a strain obtained by tissue isolation of white jade mushroom 'B-4' from Tianjin Lvsheng Pengyuan Agricultural Technology Development Co., Ltd. through fruiting body tissue isolation method.

[0026] The main planted mushrooms 'Hy0051' and 'Hy0056' were selected as hybrid parents, and 10 monospore strains with faster growth and more developed aerial hyphae were selected from each of the two ...

Embodiment 2

[0033] Example 2 The SV detection technology was used to compare the DNA difference between this strain and its parent.

[0034] Test materials: 3 strains of jiji mushrooms, among which the strain to be tested is 'Fuhong No. 1', and the 2 control strains are 'Hy0051' and 'Hy0056' respectively.

[0035] Inspection method: According to the genome sequencing data, Kmer subtractive assembly technology is used to obtain differential fragments (SV), and several SV sequences are selected accordingly to design specific primers to amplify the samples to be tested. (For references see Alkan, C., Coe, B. P. & Eichler, E. E. Genome structural variation discovery and genotyping . Nature reviews. Genetics, 2011 (12), 363-376; Fengli Zhu et al., Whole Genome Resequencing Analysis of Korean Chizhi, World Science and Technology-Modernization of Traditional Chinese Medicine, 2019, 21(4)764-774.). SV test primer sequences were synthesized by Qingke Biotechnology Co., Ltd.

[0036] Mycelia act...

Embodiment 3

[0043] The multi-batch pilot test yield results of 'Fuhong 1' showed that 'Fuhong 1' met the requirements of industrial cultivation, and its yield was higher than that of its parents.

[0044] Participating strains: Fuhong 1', 'Hy0056'.

[0045] Test location: Tianjin Binhai Desheng Agricultural Technology Co., Ltd.

[0046] Test arrangement: The strains are uniformly produced, and the test sites are cultivated and managed in accordance with local production management methods.

[0047] Test results: The test found that the hyphae of 'Fuhong 1' were thicker in PDA, the optimum growth temperature was 20°C, and the growth rate was 0.24 cm / d, which was 14 times faster than that of the parents 'Hy0056' and 'Hy0051' % and 7%. The cultivation results of ‘Fuhong 1’ are as follows: image 3 shown.

[0048] Cultivation findings: (1) The time from inoculation to harvest, 'Fuhong 1' was the same as that of the parent; (2) The average yield of 'Fuhong 1' was 230.0 g / bottle, and the av...

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Abstract

The invention provides a hypsizygus marmoreus strain Fuhong No.1. The hypsizygus marmoreus strain Fuhong No.1 is preserved in China General Microbiological Culture Collection Center on April 29, 2020,and the preservation number is CGMCC NO. 19637. The 'Fuhong No.1' strain has excellent hypsizigus marmoreus strain quality characteristics of sporocarp clustered type, difficult generation of russule, round mushroom caps, white middle parts and edges, clear marbling, white gill, wavy shape, low density, white stipes, middle solid, no fluff and the like, and the average fresh mushroom yield of the'Fuhong No.1' strain in a productive test reaches 250.0 g / bottle. The defects that Hy0051 pileus is small and thin and Hy0056 stipes are thin are overcome, and the method has a good popularization prospect.

Description

technical field [0001] The invention relates to a true jiji mushroom strain Fuhong No. 1, which belongs to the field of edible fungi. Background technique [0002] Mushroom [ Hypsizygus marmoreus (Peck) H.E.Bigelow] belongs to Basidiomycotina, Laminomycetes, Agaricaceae, White Mushrooms, and Yuji. Because it has a unique crab flavor, some people call it crab-flavored mushroom, Xiuzhen mushroom, Seafood mushrooms. Fujian Province is the main planting area of ​​Shiji mushroom in southern China. The daily output of Shunchang, Fujian Province has reached 200 tons, and there are 56 seafood mushroom factory production enterprises, accounting for more than 65% of the national daily output. With the expansion of the cultivation scale of shimeji mushrooms, there are different criteria for judging jiji mushrooms in the market, such as the cultivation period, the thickness and pattern of the cap, the thickness and thickness of the stipe, whether it is hollow or not, and the presence...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12R2001/645C12N1/145
Inventor 谢宝贵班立桐刘芳蔡鹏岑光红赖丽红江益民伊格禧陈启永
Owner FUJIAN AGRI & FORESTRY UNIV
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