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Bacteroides thetaiotaomicron and application thereof

A kind of Bacteroides polymorpha, the technology of use, applied in the field of microbiology, can solve the problems of losing structure and sensory properties, reducing the immune activity of allergens, and not fundamentally alleviating or controlling crustacean food allergy

Active Publication Date: 2020-12-11
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Food allergen removal methods can significantly reduce the immune activity of allergens, but they cannot fundamentally alleviate or control crustacean food allergy, and it is easy to make crustacean food lose its original structure and sensory properties

Method used

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  • Bacteroides thetaiotaomicron and application thereof
  • Bacteroides thetaiotaomicron and application thereof
  • Bacteroides thetaiotaomicron and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The isolation and identification of embodiment 1 bacterial strain

[0056] 1. Isolation of strains

[0057] (1) Collect fecal samples from healthy people (take some students of Zhejiang Gongshang University as the object, conduct questionnaires on the students' physical health, living and eating habits, and dietary hygiene conditions, and collect healthy people who have not taken drugs for more than one year Fecal samples from healthy students), fresh feces were stored in sterile EP tubes, refrigerated at 4°C, and shipped to the laboratory within 4 hours to complete sample processing and culture. Smash the feces sample with a sterile glass rod, oscillate evenly, dilute the sample with sterile normal saline according to the ten-fold dilution method, and take 10 -3 -10 -8 The dilution solution was poured onto the solidified selective GAM (Gifuanaerobic medium) broth medium, coated evenly, placed in an anaerobic incubator, and incubated anaerobically at 37° C. for 48 hou...

Embodiment 2

[0075]Bacteroides polymorpha (WF-1198-1-2) relieves tropomyosin-sensitized BALB / c mice

[0076] (1) Establishment of Bacteroides polymorpha intervention food allergic mouse model: 15 6-week-old SPF female BALB / c mice were randomly divided into three groups: blank group, sensitization group, and treatment group. The mice were pre-raised for one week, and were sensitized by oral administration of tropomyosin (TM) on the seventh day, in which the control group (PBS), the sensitization group TM (200 μg), the treatment group TM (200 μg) + Bacteroides polymorpha Suspension (1×10 8 cfu / mouse per day), three times a week for two consecutive weeks. Afterwards, the TM corresponding to 6 times the sensitizing dose was given to challenge, once a week, for 2 consecutive weeks, and the Bacteroides polymorpha treatment group was given oral administration from the 7th day, and the experiment lasted for four weeks. Breed at 25°C, drink water freely, weigh once a week, fast after the last cha...

Embodiment 3

[0090] Bacteroides polymorpha (WF-1198-1-2) relieves tropomyosin-sensitized BALB / c mice

[0091] (1) Establishment of Bacteroides polymorpha intervened food allergic mouse model: conventional tropomyosin (TM) was used to sensitize the food allergic mouse model, and Bacteroides polymorpha suspension (1× 10 11 cfu / mouse per day) for intragastric administration to establish a mouse model of Bacteroides polymorpha intervening in food allergy. The experiment lasted for four weeks, fasted after the last challenge, and executed by neck breaking the next day.

[0092] (2) Weekly body weight change of the mice: During the feeding period of the mice, the mice were weighed on the same day every week, and the body weight changes of the mice were observed. And calculate the weekly body weight growth rate of each group of mice, the calculation formula:

[0093]

[0094] The result is as Figure 8 As shown, after TM sensitization, the weight of the mice decreased, but the weight of th...

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Abstract

The invention provides application of bacteroides thetaotaomicron to preparation of a food or medicine for preventing, relieving or treating allergy to tropomyosin in foods; the bacteroides thetaiotaomicron is collected in China Center for Type Culture Collection on April 30, 2020, and the collection number is CCTCC M 2020105. The bacteroides thetaotaomicron can regulate the imbalance of Th1 / Th2 and Th17 / Treg cell subsets, so that the allergic reaction caused by the tropomyosin in the foods can be relieved; immune diseases including food allergy, bronchial asthma and the like are relieved; andthe bacteroides thetaotaomicron has high pertinence, high economic benefits, and few side effects, and the raw materials are green, environment-friendly and pollution-free.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a Bacteroides polymorpha and its use. Background technique [0002] Aquatic products are rich in nutrition and delicious, and are deeply loved by people. They are an important part of global food consumption. China has been the largest country in aquaculture and consumption for many years, and its aquaculture and consumption have continued growing trend. However, crustaceans such as shrimp and crab, as one of the eight major food allergens, often cause severe food allergic reactions in humans, posing a huge threat to people's lives and health. Among them, Tropomyosin (TM) widely exists in crustaceans and is the main allergen of crustaceans. It has high thermal stability, high sequence conservation, and obvious cross-reaction between species. Patients with allergies may experience allergic symptoms such as redness, rhinitis, and asthma. In severe cases, they may be accompanied by co...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K35/741A61P11/06A61P37/08C12N1/20C12R1/01
CPCA23L33/135A61K35/741A61P37/08A61P11/06A23V2002/00C12R2001/01C12N1/205A23V2200/30A23V2200/324
Inventor 傅玲琳王彦波王翀王方婷周瑾茹张巧智
Owner ZHEJIANG GONGSHANG UNIVERSITY
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