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Detection and identification method for acanthocephalan worm of neoechinorhynchus

An identification method and technology of acanthocephalus, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem that the species cannot be determined, the targeted protection and control work cannot be carried out smoothly, and cannot be completely effective and accurate The problem of distinguishing species and other issues can be achieved to improve the accuracy rate, the experimental results are accurate and reliable, and the accuracy rate is high.

Pending Publication Date: 2020-12-04
西藏自治区农牧科学院水产科学研究所
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  • Application Information

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Problems solved by technology

That is to say, the identification of universal primers cannot completely effectively and accurately distinguish species, the species cannot be determined, and targeted protection and control work cannot be carried out smoothly.

Method used

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  • Detection and identification method for acanthocephalan worm of neoechinorhynchus

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Experimental program
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Effect test

Embodiment 1

[0031] A method for detection and identification of the genus Acanthocephala, comprising the steps of:

[0032] A, according to the morphological characteristics, collect the new Acanthocephala genus Acanthocephala in the fish host;

[0033] B. Extract the DNA of the parasite sample;

[0034] C. Carry out agarose gel electrophoresis to detect the quality of the extracted DNA, if the quality is qualified, then set up a reaction system and carry out PCR amplification;

[0035] D. Sequence comparison is carried out on the sequencing results, and if the consistency is above 99%, it is determined that the species is the Acanthocephala genus Neoacantha.

Embodiment 2

[0037] This embodiment is on the basis of embodiment 1:

[0038] The collection method of the worm body sample is to scrape the contents and mucus of the digestive tract of fish, place them in a glass plate, and squeeze them with a glass plate of the same size to disperse the contents and mucus, and observe the worms with a portable dissecting microscope. body shape and collect the acanthocephala.

[0039] The size of the glass plate is 10*10 cm, which is suitable for the portable dissecting mirror used.

Embodiment 3

[0041] This embodiment is on the basis of embodiment 1:

[0042] The morphological characteristics of the genus Acanthocephala, white or light yellow, with smooth body surface without spines, banana-like in shape, slightly curved to the ventral surface, the rear part of the body is slightly thinner than the front part of the body, and the body length is about 100 inches wide. More than 3 times, females are larger than males. Elliptical giant nuclei can be seen in the body wall, with 1 on the ventral surface and 4 to 5 on the back. The snout is short and small, in the shape of an enlarged ball. The hooks are arranged in a spiral shape, 3 rows, 6 hooks in each row, 18 hooks in total; the hooks in the first row are wider, and the hooks in the second and third rows are equal in size, smaller than the hooks in the first row. There are two rod-shaped snout glands, one with 1 meganucleus and the other with 2 meganuclei; the length of snout glands varies greatly among individuals, t...

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Abstract

The invention provides a detection and identification method for an acanthocephalan worm of neoechinorhynchus. The method comprises the following steps that according to morphological characteristics,the acanthocephalan worm of neoechinorhynchus in a fish host is collected; DNA of a worm body sample is extracted; agarose gel electrophoresis is carried out to detect the quality of the extracted DNA, if the quality is qualified, a reaction system is established, and PCR amplification is carried out; and a sequencing result is subjected to sequence alignment, and if the consistency is 99 % or above, it is judged that the species is the acanthocephalan worm of neoechinorhynchus. The identification method has the advantage that the experimental results are accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of identification by combining traditional morphology and molecular biology detection, and in particular relates to a detection and identification method for Acanthocephala genus Neoacantha. Background technique [0002] The Acanthocephala genus Neoacantha is a common fish parasite in Tibet. It penetrates the host's intestinal mucosa by its snout to live as a parasite. A large number of parasitism can damage the intestinal wall of the host, cause inflammation, endanger the health of fish, and even cause intestinal perforation, intestinal blockage, make fish thin, cause various diseases, and even cause fish death in severe cases. The development of fishery is accompanied by the struggle against various diseases of fish. It is necessary to accurately identify the infected parasites before formulating a reasonable and effective treatment plan. This is the basis and prerequisite for the sustainable and healthy d...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888
Inventor 潘瑛子曾本和王金林
Owner 西藏自治区农牧科学院水产科学研究所
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