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Method for extracting and preparing high-content dihydromyricetin from ampelopsis grossedentata

A technology of dihydromyricetin and high content is applied in the preparation of alcoholic beverages, food ingredients containing natural extracts, functions of food ingredients, etc., and can solve the problems of low yield of dihydromyricetin, long separation and purification period, etc., Achieve the effect of shortening production cycle, high product yield and mild conditions

Pending Publication Date: 2020-12-04
劲牌持正堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the preparation methods of high-content dihydromyricetin include macroporous resin purification, aqueous phase recrystallization and other processes, the separation and purification cycle is long, and the extraction and preparation process is likely to cause low yield of dihydromyricetin

Method used

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  • Method for extracting and preparing high-content dihydromyricetin from ampelopsis grossedentata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Extraction: Take 20 kg of rattan tea medicinal materials, add 10 times the amount of water, and extract twice at 70 ° C, each time for 1 hour;

[0031] (2) Filtration: filter the extract from the medicinal residues while hot through a 40-mesh duplex filter, and collect the clear liquid;

[0032] (3) Cooling: the filtrate to be extracted is cooled for 6 hours, and the temperature is cooled to 20°C to enter the next step;

[0033] (4) Solid-liquid separation: the cooling liquid is separated from the solid and liquid by a decanter centrifuge, and the precipitate is collected to be the crude extract of rattan tea, the content of dihydromyricetin is 82%, and the yield is 32%;

[0034] (5) One-step reverse-phase recrystallization: heat and dissolve the crude extract of vine tea with 82% content with 25 times of 70% ethanol for 1 hour, filter with a 30nm ceramic membrane, and dilute the filtrate to 10% ethanol concentration with water to carry out one-step reverse-phase re...

Embodiment 2

[0038] (1) Extraction: take 30 kg of rattan tea medicinal materials, add 15 times the amount of water, and extract twice at 80°C, each time for 1.5 hours;

[0039] (2) Filtration: filter the extract from the medicinal residues while hot through a 50-mesh duplex filter, and collect the clear liquid;

[0040] (3) Cooling: the filtrate to be extracted is cooled for 8 hours, and the temperature is cooled to 30°C to enter the next step;

[0041] (4) Solid-liquid separation: the cooling liquid is separated from the solid-liquid by a decanter centrifuge, and the precipitate is collected to be the crude extract of rattan tea, the content of dihydromyricetin is 83%, and the yield is 31%;

[0042] (5) One-step reverse-phase recrystallization: heat and dissolve the 83% vine tea crude extract with 28 times 75% ethanol for 1.5 hours, filter with a 40nm ceramic membrane, and dilute the filtrate with water to 13% ethanol concentration for one-step reverse-phase recrystallization. Stand for ...

Embodiment 3

[0046] (1) Extraction: Take 40 kg of rattan tea medicinal materials, add 20 times the amount of water, and extract 3 times at 90 ° C, each time for 2 hours;

[0047] (2) Filtration: filter the extract from the medicinal residues while hot through a 60-mesh duplex filter, and collect the clear liquid;

[0048] (3) Cooling: the filtrate to be extracted is cooled for 9 hours, and the temperature is cooled to 40°C to enter the next step;

[0049] (4) Solid-liquid separation: the cooling liquid is separated from the solid-liquid by a decanter centrifuge, and the precipitate is collected to be the crude extract of rattan tea, the content of dihydromyricetin is 83%, and the yield is 33%;

[0050] (5) One-step reverse-phase recrystallization: the vine tea crude extract with 83% content was heated and dissolved with 30 times 80% ethanol for 2 hours, filtered through a 50nm ceramic membrane, and the filtrate was diluted with water to 15% ethanol concentration to carry out one-step rever...

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Abstract

The invention discloses a method for extracting and preparing high-content dihydromyricetin from ampelopsis grossedentata, which is used for preparing the high-content dihydromyricetin through the steps of extraction, separation, ethanol recrystallization and the like. Only solvent water and ethyl alcohol are used, and the method has the advantages of being free of pollution, simple in process, convenient to operate, high in benefit and the like and is suitable for production of dihydromyricetin.

Description

technical field [0001] The invention relates to the technical field of biomass extraction, in particular to a method for extracting high-content dihydromyricetin. Background technique [0002] Vine tea is a kind of wild vine in the family Vitaceae. Its scientific name is Snake-toothed Grape. It is mainly distributed in Hubei, Hunan, Jiangxi, Yunnan, Guizhou and other provinces. Sore throat, jaundice hepatitis and other diseases. [0003] Vine tea contains a large amount of flavonoids, the main substance is dihydromyricetin, and the content in the effective part is even as high as 27%. The high content of flavonoids makes it have a good preventive effect on cardiovascular diseases such as arrhythmia, myocardial ischemia, hypertension, coronary heart disease, and atherosclerosis. At the same time, vine tea is rich in dietary fiber, carotene and vitamin E, so it has a good preventive effect on cancer. The vine tea extract is rich in sugar and amino acids, including 8 kinds o...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40A23L33/105C12G3/055
CPCC07D311/32C07D311/40A23L33/105C12G3/055A23V2002/00A23V2200/30A23V2250/21
Inventor 江丹刘晶晶袁咏红陈志元孙代华李强
Owner 劲牌持正堂药业有限公司
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