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A kind of method for preparing agarose

A technology of agarose and colloid, applied in the field of preparation of agarose, can solve the problems of long production cycle, high cost, cumbersome production process, etc., achieve the effect of improving sample quality, improving gel strength, and reducing intermediate production links

Active Publication Date: 2022-07-19
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has certain disadvantages in the process of producing agarose. The raw material agar often needs to be extracted from seaweed by traditional alkali treatment process to make agar powder and then further modified. There are problems such as long production cycle, cumbersome production process and high cost.

Method used

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  • A kind of method for preparing agarose
  • A kind of method for preparing agarose
  • A kind of method for preparing agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Different maleic anhydride addition amounts

[0046] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0047] Acidification treatment: After the step of alkali treatment, the Grapefruit was added with sulfuric acid, oxalic acid and EDTA in the proportions of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40min, poured off the liquid, soaked and rinsed until the pH of the lotion was neutral.

[0048] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0049] Bo...

Embodiment 2

[0054] Example 2 at different temperatures

[0055] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0056] Acidification treatment: After the step of alkali treatment, the Grapefruit was added with sulfuric acid, oxalic acid and EDTA in the proportions of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40min, poured off the liquid, soaked and rinsed until the pH of the lotion was neutral.

[0057] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0058] Boiled gelatin: After w...

Embodiment 3

[0063] Example 3 Different pH

[0064] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0065] Acidification treatment: After the step of alkali treatment, the Grapefruit was added with sulfuric acid, oxalic acid and EDTA in the proportions of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40min, poured off the liquid, soaked and rinsed until the pH of the lotion was neutral.

[0066] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0067] Boiled gelatin: After washing, the g...

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Abstract

The present invention relates to a method for preparing agarose, which comprises the following steps: after washing grass, performing alkali treatment, acidification treatment, bleaching treatment, boiling gelatin, filtering, cooling and solidifying, so as to obtain colloid; ; Add water to the crushed colloid, heat, adjust pH, and then slowly add maleic anhydride ethanol solution dropwise to react, filter after the reaction, use absolute ethanol and deionized water to alternately wash the colloidal sample, and repeatedly wash until The pH of the lotion is neutral, and the colloidal sample is then frozen, dehydrated, dried and pulverized to obtain agarose. The method can significantly reduce the content of sulfuric acid groups in the agar, increase the gel strength, and significantly reduce the electroendosmosis, so as to meet the requirements of the commercially available agarose index, the reaction time is short, and the environment is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of deep processing of seaweed, in particular to a method for preparing agarose. Background technique [0002] Agarose is a polysaccharide polymer, the main component of agar, its basic structure is repeating alternating 1,3-linked β-D-galactose and 1,4-linked 3,6-anhydro-α-L - Galactose. Agarose is white or slightly yellow powder, odorless, tasteless, soluble in hot water, and soluble in dimethyl sulfoxide (DMSO). Compared with agar, agarose contains less negatively charged groups and has better properties. It has the characteristics of high gel strength, low sulfate content, and low electro-endosmosis. These properties also make agarose in the fields of biology and medicine. Wide range of applications. [0003] In the 1960s, a scientist in Sweden isolated agarose for the first time. Since then, researchers from various countries have begun to explore the preparation technology of agarose. At present, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/12
CPCC08B37/0039C08B37/0003
Inventor 肖安风张聪叶思莹张永辉肖琼杨秋明陈福泉
Owner SHANGHAI OPM BIOSCI CO LTD
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