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Method for preparing agarose

A kind of technology of agarose and colloid, which is applied in the field of preparation of agarose, can solve the problems of long production cycle, high cost, complicated production process, etc., and achieve the effect of reducing intermediate production links, saving production cost and shortening production cycle

Active Publication Date: 2020-11-27
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has certain disadvantages in the process of producing agarose. The raw material agar often needs to be extracted from seaweed by traditional alkali treatment process to make agar powder and then further modified. There are problems such as long production cycle, cumbersome production process and high cost.

Method used

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  • Method for preparing agarose
  • Method for preparing agarose
  • Method for preparing agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 different maleic anhydride additions

[0046] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0047] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0048] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion...

Embodiment 2

[0054] Embodiment 2 different temperatures

[0055] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0056] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0057] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral. ...

Embodiment 3

[0063] Example 3 Different pH

[0064] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) to water, and Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0065] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0066] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral.

[0067] Boil...

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Abstract

The invention relates to a method for preparing agarose. The method comprises the following steps of: cleaning gracilaria, and carrying out alkali treatment, acidification treatment, bleaching treatment, colloid boiling, filtration, cooling and solidification to obtain colloid; stirring and crushing the colloid; and adding water into the crushed colloid, heating, adjusting the pH value, slowly dropwise adding a maleic anhydride ethanol solution for reaction, performing filtering after finishing the reaction, alternately washing the colloidal particle sample by using absolute ethyl alcohol anddeionized water, repeatedly washing the sample until the pH value of the washing solution is neutral, and freezing, dehydrating, drying and crushing the colloidal particle sample to obtain agarose. According to the method, the content of sulfuric acid groups of agar can be remarkably reduced, the jelly strength is improved, the electroendosmosis is remarkably reduced, the index requirements of thecommercially available agarose is met, the reaction time is short, and the method is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of seaweed deep processing, in particular to a method for preparing agarose. Background technique [0002] Agarose is a polysaccharide polymer and the main component of agar. Its basic structure is repeated alternating 1,3-linked β-D-galactose and 1,4-linked 3,6-anhydro-α-L - Galactose. Agarose is white or slightly yellow powder, odorless and tasteless, and has good solubility in hot water and can also be dissolved in dimethyl sulfoxide (DMSO). Compared with agar, agarose contains less negatively charged groups and has better properties. It has the characteristics of high gel strength, low sulfate content, and low electroosmosis. These properties also make agarose have great potential in the fields of biology and medicine. Wide range of applications. [0003] In the 1960s, a scientist in Sweden isolated agarose for the first time. Since then, researchers from various countries have begun to explore the te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/12
CPCC08B37/0039C08B37/0003
Inventor 肖安风张聪叶思莹张永辉肖琼杨秋明陈福泉
Owner SHANGHAI OPM BIOSCI CO LTD
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