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Skin cold storage liquid and application thereof

A technology for preserving liquid and skin, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of unclear components, complex and diverse components, etc., to maintain skin moisture, improve the acquisition of fibroblasts, and save time. long effect

Pending Publication Date: 2020-11-24
北京利物谱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since calf serum refers to the light yellow clear viscous liquid left after the fibrin is removed from fetal calf blood, its components are complex and diverse, and some components are not clear, so there are many potential safety problems in the application of preserved skin

Method used

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  • Skin cold storage liquid and application thereof
  • Skin cold storage liquid and application thereof
  • Skin cold storage liquid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Using sterile water as the solvent, according to hydroxyethyl starch 50g / L, tretinoin 1g / L, uronic acid 3g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 1g / L, Y-276322HCl 10μmol / L (μM) to make up the solution, then adjust the pH to 7.0 using sodium hydroxide.

Embodiment 2

[0017] Using sterile water as a solvent, according to 45g / L of hydroxyethyl starch, 1.2g / L of retinoic acid, 2.5g / L of uronic acid, 2.5g / L of potassium dihydrogen phosphate, 0.9g / L of magnesium sulfate, Y- 276322HCl 3μmol / L to prepare the solution, and then use sodium hydroxide to adjust the pH to 6.9.

Embodiment 3

[0019] Using sterile water as a solvent, according to hydroxyethyl starch 55g / L, tretinoin 0.8g / L, uronic acid 3.5g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.2g / L, Y- 276322HCl 18μmol / L to prepare the solution, and then use sodium hydroxide to adjust the pH to 7.2.

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PUM

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Abstract

The invention discloses skin cold storage liquid and application thereof. The cold storage liquid comprises hydroxyethyl starch, tretinoin, uronic acid, monopotassium phosphate, magnesium sulfate, sodium hydroxide and an ROCK inhibitor, wherein the ROCK inhibitor is selected from one or two of Y-27632 and Y-27632 2HCl. The cold storage liquid provided by the invention can be used for human skin storage, so that the skin can keep biological activity, and under the in-vitro condition, skin cells can continuously survive for a long time. The requirement of stably storing the skin for a long timecan be met, and meanwhile, the skin can survive after being stored and can be cultured into a large number of fibroblasts.

Description

technical field [0001] The invention relates to the field of skin preservation, in particular to a skin cryopreservation solution and its application. Background technique [0002] At present, low-temperature cryopreservation of skin is mostly used in China. The main defect is that the recrystallization produced in the rewarming process will cause harmful ice crystal damage to the skin. Studies have shown that ice crystal damage can be avoided only when the rewarming speed reaches 3.3-17°C / s. Such a fast rewarming speed is difficult to achieve in clinical application. Dimethyl sulfoxide is often added as a cell protectant when cryopreserving skin. Because of its antifreeze effect, it is used for the protection of cell cryopreservation. Dimethyl sulfoxide can lower the freezing point of cells, reduce the formation of ice crystals, reduce the damage of free radicals to cells, and change the passage of biofilms to electrolytes, drugs, poisons and metabolism. permeability. Ho...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 孙浩蔡军聂宵张鹏
Owner 北京利物谱生物科技有限公司
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