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Method for preparing agarose

A technology of agarose and glue block, which is applied in the field of agarose preparation, can solve the problems of complicated production process, high cost, long production cycle and the like, and achieves the effects of improving desulfurization efficiency, preventing degradation and reducing intermediate production links.

Active Publication Date: 2020-11-20
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has certain disadvantages in the process of producing agarose. The raw material agar often requires seaweed to produce agar powder through the traditional alkali treatment process and then further modified. There are problems such as long production cycle, cumbersome production process, and high cost.

Method used

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  • Method for preparing agarose
  • Method for preparing agarose
  • Method for preparing agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Different glutaric anhydride and ethanol solid-liquid ratio of embodiment 1

[0044] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at the ratio of 1g:20mL (m:v) algal water, Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0045] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0046] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse...

Embodiment 2

[0053] Embodiment 2 different temperatures

[0054] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at the ratio of 1g:20mL (m:v) algal water, Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0055] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0056] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral. ...

Embodiment 3

[0063] Example 3 Different pH

[0064] Alkali treatment: Rinse Gracilaria tap water, dry naturally, take 100g of dried Gracilaria, add 7% (w / v) NaOH solution at the ratio of 1g:20mL (m:v) algal water, Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, soak and rinse with tap water, with an interval of 40 minutes each time, until the pH of the washing solution is neutral.

[0065] Acidification treatment: add sulfuric acid, oxalic acid and ethylenediaminetetraacetic acid to the Gracilaria after the alkali treatment step according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system Disodium, treated for 40 minutes, poured off the liquid, soaked and rinsed until the pH of the washing liquid was neutral.

[0066] Bleaching treatment: add water to the acidified Gracilaria, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 minutes, then soak and rinse until the pH of the lotion is neutral.

[0067] Coo...

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Abstract

The invention relates to a method for preparing agarose. The method comprises the following steps: cleaning gracilaria, and carrying out alkali treatment, acidification treatment, bleaching treatment,gelatin boiling, filtration, cooling and solidification to obtain colloid; dicing the colloid, and mixing the diced colloid with an ethanol solution to obtain a colloid block mixed solution; and heating the colloid block mixed solution, dropwise adding a glutaric anhydride ethanol solution for reaction, adjusting the pH value in the reaction process, filtering after the reaction is finished to obtain colloid blocks, and washing, dehydrating, drying and crushing the colloid blocks to obtain the agarose. According to the method, the content of sulfuric acid groups of agar can be remarkably reduced, the gel strength is improved, the electroendosmosis is remarkably reduced, the commercially available agarose index requirement is met, the reaction time is short, and the method is environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of seaweed deep processing, in particular to a method for preparing agarose. Background technique [0002] Agar is mainly composed of two parts: agarose and agar pectin. Among them, agarose is the main component of agar gel, and its structure is a long chain alternately linked by 1,3-β-D-galactose and 1,4-α-3,6-endether-L-galactose composed of hydrophilic polymers. Due to the few oxygen-containing groups on its structural units, it has excellent characteristics such as low sulfate content, high gel strength, low electroosmosis, etc., making agarose more and more popular as an electrically neutral polysaccharide. It is an ideal inert carrier and has been widely used in gel electrophoresis, affinity chromatography, molecular sieve, immunology, biomedical research, etc. Compared with agar with a higher content of sulfate groups, agarose is more widely used in microbial experiments, and its selling price is a...

Claims

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Application Information

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IPC IPC(8): C08B37/12
CPCC08B37/0039C08B37/0003
Inventor 肖安风叶思莹陈福泉杨秋明肖琼张永辉
Owner SHANGHAI OPM BIOSCI CO LTD
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