Multi-functional hybrid peptide with antibacterial, anti-inflammatory and detoxifying activities and applications thereof

Abstract

The invention relates to the technical field of protein engineering, and specifically relates to multi-functional hybrid peptide with antibacterial, anti-inflammatory and detoxifying activities and applications thereof. The multi-functional hybrid peptide is named as CLA and obtained by immune anti inflammatory hybrid peptide LTA and antibacterial and antiviral hybrid peptide CL through protein engineering computer design, heterozygosis and in vivo and in vitro re-screening; and the amino acid sequence is shown as SEQ ID NO. 1. The multi-functional hybrid peptide has high antibacterial, anti-inflammatory and anti infective functions, can dispel endotoxin and adjust immunity, and is low in cytotoxicity and high in safety, so that the multi-functional hybrid peptide can be used as ideal antibiotic substitutes, biological antibacterial drugs for human and animals, anti-infective drugs, feed additives, preservatives, disinfectants, immunomodulator or anti-inflammatory agents; and therefore, the multi-functional hybrid peptide has good application potential and values.

Description

technical field [0001] The invention relates to the technical field of protein engineering, in particular to a novel multifunctional hybrid peptide with antibacterial, anti-inflammatory and interpretation activities and its application. Background technique [0002] Bacterial infection is an infectious disease caused by pathogenic bacteria or conditional pathogenic bacteria invading the animal body to grow and reproduce, producing toxins (such as endotoxin) and other metabolites, which can induce inflammation and oxidative damage, etc., and damage the immune system in severe cases. even lead to death. When animals are young, weak, sick, stressed, or infected with pathogens, their immunity is often reduced, and then secondary infections (bacterial infections, etc.) occur, causing inflammatory reactions. For the treatment of infection and inflammation, the traditional control strategy is to use antibiotics and chemical drug agents. The continuous use of antibiotics and chemi...

AI Technical Summary

Problems solved by technology

[0005] However, natural bioactive peptides also have some defects, mainly as follows: (1) Some natural bioactive peptides have relatively low biological activity and single function, such as the problem of weak antibacterial activity of pig lactoferrin peptide ; and although cecropin A has good antibacterial efficacy, its anti-inflammatory ability is relatively weak, so it is difficult for natural bioactive peptides to completely replace antibiotics; While killing pathogenic microorganisms, it will also cause serious damage to the body (such as melittin and LL-37); (3) Most natural active peptides have poor stability in vivo, short half-life, and are easily degraded (gastrointestinal loss) , which increases the difficulty of its practical application

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