Precious metal nanocone SERS substrate for detecting novel coronavirus and preparation method

Abstract

The invention belongs to the technical field of laser Raman spectroscopy and virus detection, and particularly relates to a precious metal nanocone SERS substrate for detecting a novel coronavirus anda preparation method. The SERS substrate comprises a substrate body, a nanocone array located on the substrate body and a precious metal film wrapping the nanocones and the surface of the substrate body where the nanocones are located, the nanocones forming the nanocone array are radial from the center of the substrate body to the periphery, the included angle between every two adjacent nanoconesis gradually changed, and coronaviruses with the size of 60-140 nm can be effectively captured. the substrate has strong compatibility, and when the virus is in contact with the substrate and slideson the surface, the capture efficiency can be greatly improved. The preparation method comprises the following steps: covering a substrate with a single-layer polystyrene microsphere template, bendingthe substrate, carrying out reactive ion etching, carrying out magnetron sputtering on a noble metal film, and leveling the substrate. The preparation method is simple, and the prepared noble metal nanocone has three-dimensionally distributed SERS hot spots and high SERS sensitivity.

Description

technical field [0001] The invention relates to the technical field of laser Raman spectroscopy and virus detection, in particular to a noble metal nanocone SERS substrate and a preparation method for detecting novel coronaviruses. Background technique [0002] The 2019 novel coronavirus (2019-nCOV) can be transmitted through respiratory droplets, contact, aerosol, etc. After infection, the symptoms are mainly fever, cough, shortness of breath and dyspnea, and severe cases can lead to renal failure or even death. Due to the lack of targeted specific drugs, physical isolation of suspected and confirmed cases is an effective way to prevent further deterioration of the epidemic. Therefore, it is particularly important to quickly screen patients who have been infected with new pneumonia from the population. At present, the commonly used screening and detection technology is mainly reverse transcription-polymerase chain reaction (RT-PCR). , has a high false negative rate. Ther...

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Problems solved by technology

However, in order to capture the virus, it is necessary to design an auxiliary reagent containing a replace chain, a capture chain, and a probe chain based on the viral gene sequence, and it is necessary to co-culture the detection sample with the auxiliary reagent at a certain temperature. The capture method steps are cumbersome and time-consuming

At the same time, the traditional noble metal cone-shaped SERS substrate has a definite nanocone spacing and gap, which can only capture viruses with a small size distribution, and the capture efficiency is low, resulting in weak Raman signal intensity required for detection.

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