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Application of combination of morroniside and stem cells to preparation of cartilage repair materials

A technology of cartilage repair and morroniside, which is applied in the fields of medical science, tissue regeneration, prosthesis, etc., can solve the problems of limited application, poor effect, and complications of cartilage donor sites, and achieve the goal of promoting repair and repairing the damaged area Effect

Inactive Publication Date: 2020-11-03
ZHONGSHAN HOSPITAL XIAMEN UNIV
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Problems solved by technology

[0008] 5. Anti-tumor effect: Morroniside can inhibit the proliferation of human malignant melanoma cells and promote their apoptosis, and it is used as an effective component in anti-thyroid tumor and anti-bladder cancer drugs, but its specific biological effects remain to be studied
[0009] 6. Others: Morroniside is also used as an effective component in the treatment of mumps, bronchitis, bronchial asthma, chronic renal failure drugs and labor pain drugs, but its specific biological role in related drugs remains to be studied
Bone marrow stimulation surgery, such as trepanation decompression, is effective in the treatment of small cartilage injuries in young patients, but less effective in large cartilage injuries and elderly patients
Autologous cartilage transplantation, osteochondral transplantation and chondrocyte transplantation are also suitable for patients with small cartilage injuries, but their application is limited in larger cartilage injuries, and there are negative effects such as complications in the cartilage donor site

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  • Application of combination of morroniside and stem cells to preparation of cartilage repair materials
  • Application of combination of morroniside and stem cells to preparation of cartilage repair materials
  • Application of combination of morroniside and stem cells to preparation of cartilage repair materials

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Embodiment Construction

[0049] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0050] Some scholars have confirmed that morroniside can protect chondrocytes cultured in vitro, promote the survival of chondrocytes in an inflammatory environment, and have a therapeutic effect on osteoarthritis in rat animal models. Based on the research of these scholars, the present application cultured bone marrow-derived rat MSCs in vitro, and detected the proliferation-promoting effect of morroniside on rat MSCs and its effect on rat MSCs to chondrocytes through cell and molecular biology techniques. directional differentiation; and through the inhibitor of the non-action site, explored the molecular biology matrix for inducing rat MSC to differentiate into chondrocytes, and preliminarily judged its induction effect, acting on the target of SMAD3 in the SMAD signaling pathway The point is to promote the differentiation of MSCs into chondrocyte...

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Abstract

The invention relates to an application of combination of morroniside and stem cells to preparation of cartilage repair materials. Morroniside can promote phosphorylation of an Smad3 signal channel ofa rat MSC cultured in vitro to improve expression of the Sox9 protein level of the rat MSC without affecting the expression level of endogenous TGF-beta 3 genes of the rat MSC, TGF-beta receptors areinhibited through Ly2157299,, the phosphorylation effect of an SMAD3 signal channel of the rat MSC cultured in vitro is inhibited, and the expression of the Sox9 protein level is reduced. When the morroniside is applied to the rat MSC to which the Ly2157299 is applied, the inhibition effect of the Ly2157299 on the phosphorylation of the SMAD3 can be partially reversed. The etiology acidificationof an ERK signal channel is inhibited through U0126, and the phosphorylation effect of the SMAD3 signal channel of the rat MSC cultured in vitro is inhibited.

Description

technical field [0001] The present invention relates to the application of morroniside, in particular to morroniside, a traditional Chinese medicine extract, which induces bone marrow mesenchymal stem cells to differentiate into chondrocytes, and verifies in animals by implanting morroniside containing bone marrow mesenchymal stem cells Hydrogel can promote the application of morroniside in the repair of osteochondral injury combined with stem cells in the preparation of cartilage repair materials. Background technique [0002] Morroniside (Morroniside), molecular formula C17H26O11 ( figure 1 ), is an iridoid glycoside compound, which was first extracted from the pulp of Cornus (Cornus), a plant of the family Cornaceae (Cornaceae), and then successively found in honeysuckle (Lonicera japonica), gray felted honeysuckle (Lonicera macranthoides), elderberry Morroniside is also found in traditional Chinese medicinal materials such as Sambucus williamsii Hance. As a traditional...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/54A61L27/52A61L27/20
CPCA61L27/3834A61L27/3852A61L27/54A61L27/52A61L27/20A61L2430/06
Inventor 夏春张同恩
Owner ZHONGSHAN HOSPITAL XIAMEN UNIV
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