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Corneal graft material capable of rapidly repairing damaged tissue and preparation method thereof

A technology of damaged tissue and transplanted materials, which is applied in the field of medical devices and can solve problems such as affecting vision and blindness of patients

Active Publication Date: 2020-10-30
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, vision will be affected to varying degrees, and in severe cases, patients will be blinded due to perforation

Method used

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  • Corneal graft material capable of rapidly repairing damaged tissue and preparation method thereof
  • Corneal graft material capable of rapidly repairing damaged tissue and preparation method thereof
  • Corneal graft material capable of rapidly repairing damaged tissue and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The preparation method of the keratoplasty material capable of quickly repairing damaged tissue of the present embodiment comprises the following steps:

[0041]Step 1. After cleaning the extracted animal cornea, carry out high static pressure treatment, so that all the cells in the animal corneal tissue are broken; the sclera with a width of 2 mm remains on the animal cornea, and the pressure of the high static pressure treatment is 100 MPa. The temperature is 0°C, and the holding time is 1min;

[0042] Step 2. Place the animal cornea after the high static pressure treatment in step 1 in the cleaning solution, shake and wash it in a shaker for 24 hours, and remove the immunogenic cell components and soluble protein components; the cleaning solution contains transparent Phosphate buffer solution of sodium hyaluronate (it can also be replaced by carbonate buffer solution or normal saline), the mass concentration of sodium hyaluronate in the cleaning solution is 0.2%, the...

Embodiment 2

[0050] The preparation method of the keratoplasty material capable of quickly repairing damaged tissue of the present embodiment comprises the following steps:

[0051] Step 1, after cleaning the extracted animal cornea, carry out high static pressure treatment, so that all the cells in the animal corneal tissue are broken; the 3mm wide sclera remains on the animal cornea, and the pressure of the high static pressure treatment is 1000MPa, The temperature is 30°C, and the holding time is 20 minutes;

[0052] Step 2. Place the animal cornea treated with high static pressure in step 1 in a cleaning solution, shake and wash it in a shaker for 24 hours, and remove immunogenic cell components and soluble protein components; the cleaning solution contains glucose Phosphate buffer solution of polysaccharide (it can also be replaced by carbonate buffer solution or normal saline), the mass concentration of dextran in the cleaning solution is 1%, the temperature of the cleaning solution ...

Embodiment 3

[0057] The preparation method of the keratoplasty material capable of quickly repairing damaged tissue of the present embodiment comprises the following steps:

[0058] Step 1. Clean the extracted animal cornea and perform high static pressure treatment to break all the cells in the animal cornea tissue; the animal cornea retains a 5mm wide sclera, and the pressure of the high static pressure treatment is 500MPa. The temperature is 15°C, and the holding time is 10 minutes;

[0059] Step 2. Place the animal cornea after the high static pressure treatment in step 1 in the cleaning solution, shake and wash it in a shaker for 96 hours, and remove the immunogenic cell components and soluble protein components; the cleaning solution contains sulfuric acid Phosphate buffer solution of chondroitin (it can also be replaced by carbonate buffer solution or normal saline), the mass concentration of chondroitin sulfate in the cleaning solution is 1%, the temperature of the cleaning solutio...

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Abstract

The invention discloses a corneal graft material capable of rapidly repairing damaged tissue and a preparation method thereof. The preparation method comprises the following steps: 1, performing high-static-pressure treatment on animal corneas; 2, placing the animal corneas in a cleaning solution, and carrying out shaking cleaning in a shaking table; 3, carrying out immunoregulation treatment on the animal corneas by using an immunoregulation solution containing a cross-linking agent; and 4, conducting cutting, subjecting the cut corneas to filling and storing, and carrying out sterilizing. According to the invention, based on an immunological rejection mechanism and a modified corneal material itself, the prepared corneal graft material is free of complete cell residues, good in mechanical properties and degradation resistance and low in immunological rejection; generation and adhesion of macrophages can be controlled through the characteristics of the corneal graft material after thematerial is transplanted, so the immunological rejection is controlled; and compared with similar products, the graft success rate of the corneal graft material is greatly increased, and even compared with natural corneas, the graft success rate of the corneal graft material is also greatly increased.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a corneal transplant material capable of quickly repairing damaged tissues and a preparation method thereof. Background technique [0002] The cornea is completely transparent, located in the front of the eyeball, and has a transverse oval shape. Together with the sclera tissue, it provides special protection for the delicate eyeball contents, constitutes the first barrier of the eyeball, and completes more than 90% of the refractive function. formation is extremely important. [0003] As the first barrier of the eyeball, the cornea is vulnerable to external damage or infection caused by bacteria and other substances. The Bowman's membrane, stroma, and endothelial layers of the cornea cannot be regenerated once damaged. As a result, vision will be affected to varying degrees, and in severe cases, patients will be blinded due to perforation. Severe corneal l...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/50A61L27/3604A61L27/3691A61L27/3687A61L2430/16A61L2430/40
Inventor 樊杏利张立娟张勇杰王二浩任珂星
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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