Microcystis aeruginosa lysozyme and application thereof

A technology of Microcystis aeruginosa and Microcystis, which is applied in the field of microbial engineering and can solve problems such as water body algae pollution

Active Publication Date: 2020-10-27
HUATIAN ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a kind of microbial strain that can b...

Method used

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  • Microcystis aeruginosa lysozyme and application thereof
  • Microcystis aeruginosa lysozyme and application thereof
  • Microcystis aeruginosa lysozyme and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, screening and identification of thermotolerant bacillus Z-1

[0030] 1. Experimental materials

[0031] Activated sludge of the second sewage plant in Ma'anshan City, Anhui Province

[0032] The Microcystis aeruginosa used was purchased from the Wuhan Institute of Hydrobiology, Chinese Academy of Sciences. After the algae were activated, they were cultured at 25°C, with a light intensity of 2000lx and a light-to-dark ratio of 12h:12h.

[0033] 2. Medium

[0034] LB liquid medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L.

[0035] LB solid medium: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 20g / L.

[0036] Culture algae BG11 medium:

[0037]

[0038] 3. Screening of algalytic bacteria Take the activated sludge from the No. 2 Sewage Treatment Plant in Ma'anshan City, Anhui Province, remove the supernatant at rest, take 5mL of activated sludge, inoculate it into LB medium, and place it in a constant temperature incubator at 30°C....

Embodiment 2

[0047] The discussion of embodiment 2 algae-dissolving mode

[0048] Centrifuge the liquid-cultured Z-1 culture at 1200r / min, separate the bacteria from the supernatant, take the supernatant and filter it with a microporous membrane with a pore size of 0.22nm, wash the bacteria twice with sterile distilled water, and take the filtrate Inserted into liquid-cultured Microcystis at a volume ratio of 10%, and at the same time, bacterium was added to cultured Microcystis, and a blank algae solution was set as a control. Observe the color change of the water body every day, whether the microcystis is yellowed, and whether the algae are agglomerated. At the same time, the chlorophyll a of each group of Microcystis aeruginosa was measured.

[0049] The experiment found that in the experimental group of bacteria and algae liquid alone, the growth of algae was good, while in the experimental group of bacteria supernatant and bacteria liquid, the algae gradually turned yellow after the ...

Embodiment 3

[0051] The influence of embodiment 3 temperature on the effect of dissolving algae

[0052] Temperature is an important environmental condition for the growth and energy metabolism of strains, and has a great influence on the algicidal efficiency of strains. Determining the optimum temperature range in the application process is the key to ensuring the maximum potential of strains. The cultured bacteria were inoculated with Microcystis at a ratio of 5%, and placed in light incubators at 20°C, 30°C, 35°C, and 40°C respectively. figure 2 After one week, the algae-dissolving rate of each experimental group was measured to be 30%, 64%, 88%, and 83%, respectively.

[0053] The results show that the algae-dissolving efficiency is the highest when the temperature is around 35°C. In practical application, the outbreak of eutrophic algae generally occurs in summer when the temperature is higher.

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Abstract

The invention belongs to the technical field of microbial engineering, and particularly relates to a microcystis aeruginosa lysozyme and an application thereof; specifically, the strain is heat-resistant bacillus sporothermodurans, and the preservation name of the strain is heat-resistant bacillus sporothermodurans sporothermodurans Z-1; the invention also optimizes an applicable condition culturesystem of the strain, discusses the algae-lysing mechanism of the strain, researches the influence of parameters such as temperature, concentration of added bacterial liquid and pH value on the algae-lysing efficiency, and tests show that the strain has good dissolving and eliminating effects on microcystis aeruginosa, and mainly exerts the algae-lysing effect through extracellular products, wherein the efficiency of treating microcystis aeruginosa is highest under the conditions that the temperature is 25-40 DEG C, the illumination intensity is 1800-2200 lux, the light and dark period is (10-15) h: (10-15) h, and the pH value is 6-9, and the method can be applied to the treatment of the microcystis aeruginosa polluted water body.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a Microcystis aeruginosa lysing bacterium and its restoration application to water bodies polluted by algae. Background technique [0002] The eutrophication of the water body causes the algae to bloom in large numbers, which leads to the outbreak of algal blooms and red tides, which deteriorates the water quality, affects the function of the water body, and even threatens the safety of drinking water. The main manifestations of algae hazards are: large areas of algae cover the water surface, causing water quality corruption, causing suffocation of fish, etc.; many blue-green algae can produce algal toxins, which can cause damage to the human nervous system, causing convulsions, breathing difficulties and even respiratory failure. It may cause diarrhea, vomiting, and damage to organs such as liver and kidney; affect environmental indicators such as water...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07
CPCC12N1/20C02F3/34C12R2001/07C12N1/205
Inventor 崔倩倩孟溪骆守鹏高卫民陶文达
Owner HUATIAN ENG & TECH CORP MCC
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