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Zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and preparation method thereof

A technology of zinc glutamate and triphenylphosphine, applied in the field of zinc glutamate-coated Prussian blue nanoparticles and its preparation, can solve problems affecting the effect of photothermal therapy

Active Publication Date: 2020-10-27
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these two solutions have solved the problem of heat shock protein activity and expression, they can only target one heat shock protein, and there are many kinds of heat shock proteins that can play a role in photothermal therapy. heat therapy effect

Method used

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  • Zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and preparation method thereof
  • Zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and preparation method thereof
  • Zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Preparation of Prussian blue nanoparticles

[0047] Weigh 135mg of ferric chloride hexahydrate and 2625mg of citric acid monohydrate dissolved in 500mL ultrapure water to prepare ferric chloride solution; then weigh 210mg of potassium ferrocyanide trihydrate and 2625mg of citric acid monohydrate dissolved Prepare a solution in 500mL ultrapure water. Measure 60mL of ferric chloride solution into a beaker, slowly add 60mL of potassium ferrocyanide solution dropwise under stirring in a water bath at 60°C. After the dropwise addition, continue to stir at a constant temperature of 60°C for 1 min, then transfer to room temperature and stir for 5 min, then slowly pour 120 mL of acetone to induce crystallization, finally collect by centrifugation and wash with water 3 times, and obtain Prussian blue nanoparticles after vacuum freeze-drying.

Embodiment 2

[0048] Example 2: Preparation of Zinc Glutamate Coated Prussian Blue Nanoparticles

[0049] Weigh 3 mg of the Prussian blue nanoparticles in Example 1 and disperse them in ultrapure water, add 10 mL of 0.5 mg / mL polyvinylpyrrolidone solution, and stir for 30 min to fully mix. Then 8 mL of zinc nitrate solution with a concentration of 5 mol / L was added dropwise, and stirred for 30 min to assemble the first layer. After centrifugation, wash with water and disperse again. Add 5 mL of 5 mol / L disodium glutamate solution dropwise, stir for 30 min to assemble the second layer, centrifuge and wash with water to collect the precipitate. A total of 4 layers were assembled in this way, collected by centrifugation, washed twice with water, and vacuum freeze-dried to obtain zinc glutamate-coated Prussian blue nanoparticles.

[0050] According to the X-ray diffraction figure of present embodiment 1 and embodiment 2 product ( figure 2 ), all diffraction peak positions correspond to the ...

Embodiment 3

[0051] Embodiment 3: the load of drug

[0052] Weigh 5 mg of the zinc glutamate-coated Prussian blue nanoparticles prepared in Example 2 and disperse them in methanol, add the methanol solution of zinc nitrate to assemble the fifth layer, then add the methanol solution of 2 mg of lonidamine and stir for 2 h, centrifuge and Washed twice with water to obtain zinc glutamate-coated Prussian blue nanoparticles loaded with lonidamine.

[0053] Each weighed 38.1 mmol of triphenylphosphine and 2-aminobromoethane hydrobromide in a 100 mL round bottom flask, added 50 mL of acetonitrile and stirred at reflux for 24 h at 82°C. After the reaction, the precipitate was dried to obtain the product triphenylphosphine-aminoethane hydrobromide. Weigh 3mmol of lonidamine, 3mmol of 4-dimethylaminopyridine and 3mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in a round bottom flask, add 10 mL of dimethyl sulfoxide was stirred at room temperature for 6 h in the dark. Then 3 mm...

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Abstract

The invention discloses zinc glutamate-coated Prussian blue nanoparticles with triphenylphosphine-clonidamine coated with cancer cell membrane and a preparation method thereof. The nanoparticle comprises Prussian blue nanocore, wherein a surface of the Prussian blue nanocore is coated with at least one zinc glutamate layer, and a surface of the zinc glutamate layer at the outermost layer is provided with a loading layer loaded with triphenylphosphine-clonidamine, and the loading layer is coated with a tumor cell membrane layer. The nanoparticle has an ability to target tumor cells, has a longcirculation time in a body, can gather in mitochondria and cause dysfunction, reduces synthesis of ATP, down-regulates synthesis of a variety of heat shock proteins, causes cell apoptosis, and effectively enhances efficacy of tumor low-temperature photothermal therapy.

Description

technical field [0001] The invention belongs to the technical field of drug carriers, and in particular relates to zinc glutamate-coated Prussian blue nanoparticles loaded with triphenylphosphine-lonidamine wrapped by cancer cell membranes and a preparation method thereof. Background technique [0002] As a type of malignant tumor, cancer is a non-communicable disease with very high morbidity and mortality worldwide. According to the International Cancer Research Institute, there will be about 20 million new cancer cases in the world in 2025. At present, traditional cancer treatment methods still occupy the main position, such as chemotherapy, radiotherapy and surgery, which have the disadvantages of high recurrence rate and strong side effects. As a new type of treatment, photothermal therapy is a treatment method that uses photothermal conversion agents to quickly convert light energy into heat energy to kill tumor cells. Compared with traditional treatment methods, photo...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K31/416A61K33/26A61K41/00A61K47/46A61K47/18A61K47/69A61K49/00A61K49/22A61P35/00
CPCA61K9/5068A61K31/416A61K33/26A61K41/0052A61K47/183A61K49/0002A61K49/0013A61K49/225A61K47/6929A61P35/00A61K2300/00
Inventor 刘源岗王士斌王沛
Owner HUAQIAO UNIVERSITY
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