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sers-spr dual-mode sensor and its preparation method and application

A sensor and detection method technology, applied in the field of functional nanomaterials and biological detection, can solve the problems of insufficient sensitivity and specificity, and achieve improved detection sensitivity and specificity, strong local surface plasmon resonance enhancement effect, and excellent spatial positioning effect of ability

Active Publication Date: 2022-04-08
NANJING UNIV OF POSTS & TELECOMM
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention proposes a SERS-SPR dual-mode sensor for nucleic acid detection, which can effectively solve the problems of insufficient sensitivity and specificity in most current detection methods

Method used

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  • sers-spr dual-mode sensor and its preparation method and application
  • sers-spr dual-mode sensor and its preparation method and application
  • sers-spr dual-mode sensor and its preparation method and application

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Effect test

Embodiment 1

[0062] Embodiment 1 Preparation of silver nanohole-nanorod array substrate

[0063] 1. Silver nanohole-nanorod arrays were prepared by nanosphere etching (NSL), reactive ion etching (RIE) and physical evaporation deposition techniques. figure 1 The fabrication process of the silver nanopore-nanorod array is shown. In the first step, 500nm polystyrene (PS) microspheres are assembled on a clean glass or silicon wafer in a monolayer close-packed manner by the air-water interface method to form a microsphere template, a glass or silicon wafer. The size is 0.9×2.5em.

[0064] 2. In the second step, use O 2The plasma etches the PS microspheres on the surface of the substrate to reduce the diameter of the microspheres on the surface of the substrate. In the etching process, Trion Technology Phantom III RIE / ICP system was used to operate, the pressure was 40mTorr, the oxygen flow rate was 10sccm, the ICP power was 25W, the RF power was 10W, and the duration was 350s.

[0065] 3. P...

Embodiment 2

[0067] Example 2 Characterization of Refractive Index Response Performance of Silver Nanohole-Nanorod Array Substrate

[0068] The silver nanohole-nanorod array substrate (structure such as figure 2 shown) were soaked in different refractive index solvents: methanol (n=1.328), acetone (n=1.359), 1-hexanol (n=1.418), chloroform (n=1.446) and toluene (n=1.496), Afterwards, the polarized and non-polarized transmission spectra of the silver nanohole-nanorod array substrate were tested respectively. Under the irradiation of non-polarized, 0-degree polarized and 90-degree polarized light, the transmission spectrum of the silver nanohole-nanorod array substrate exhibits a refractive index dependence, such as image 3 shown. In addition, both the spectral profile and the peak-to-valley variation of the transmission spectrum exhibit obvious polarization dependence. According to the response of different peaks and valleys to different refractive index solvents, the response of peaks...

Embodiment 3

[0069] Example 3 Preparation of SERS-SPR dual-mode sensor

[0070] 1. Preparation of tetrahedral DNA probes. Such as Figure 5 As shown, tetrahedral DNA is formed by base complementary hybridization self-assembly of four single strands A, B, C, and D. Mix equimolar amounts (1 μM) of four DNA single strands in 300 μL TM buffer (20 mM Tris-HCl, 50 mM MgCl2, pH 8.0), mix them, and anneal (heat to 95 ° C for 5 min, then naturally cool to room temperature) ), forming tetrahedral DNA, the concentration of tetrahedral DNA in the final solution was 1 μM. Tetrahedral DNA formation was characterized by 10% polyacrylamide gel electrophoresis, Image 6 a is the electrophoretic gel map corresponding to the formation process of tetrahedral DNA. Compared with the combination of one (lane 1), two (lane 2) and three DNA strands (lane 3), four DNA strands are mixed and self-assembled to form a tetrahedron DNA moved the slowest in lane 4, indicating that tetrahedral DNA was formed successful...

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Abstract

The invention discloses a SERS-SPR dual-mode sensor for nucleic acid detection. The dual-mode sensor comprises a detection chip and a DNA probe, and the detection chip is a silver nanopore-nanorod array substrate with tetrahedral DNA modified on the surface. The invention also discloses a preparation method and a detection method of the SERS‑SPR dual-mode sensor. In the present invention, the detection chip is sequentially mixed with the liquid sample to be detected and the DNA probe solution, and a "detection chip-target DNA-DNA probe" complex is formed through complementary pairing, and then the transmission spectrum test and the SERS test are sequentially carried out. The wavelength change of the valley, the SERS spectrum and its characteristic signal intensity value realize the highly sensitive and specific dual-mode sensing detection of nucleic acids in serum. The liter level and sub-picomole level can realize the detection of nucleic acid markers in complex environments such as serum.

Description

technical field [0001] The invention belongs to the field of functional nanomaterials and biological detection, in particular to a SERS-SPR dual-mode sensor for nucleic acid detection and its preparation method and application. Background technique [0002] With the continuous development of molecular biology and medical fields, and further research on the mechanism of cancer, people have gradually realized that cancer is a disease caused by changes in genetic material, that is, the corresponding cells secrete cancer markers, including The presence or content of nucleic acid (such as microRNA), protein, hormone, etc. in the blood will reflect the status of cancer. However, in the early stage of lesions, the content of cancer markers is very low. Due to the limitation of sensitivity of conventional clinical detection techniques, it is easy to miss detection during the detection process of markers; in addition, the samples for cancer marker detection are generally blood or tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N21/552
CPCG01N21/658G01N21/553
Inventor 宋春元蒋新宇张晶晶汪联辉
Owner NANJING UNIV OF POSTS & TELECOMM
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