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A kind of biomimetic nano preparation based on platelet membrane fragments and its preparation method and application

A bionic nano-platelet technology, applied in the field of medicine, can solve the problems of inability to produce EPR effect and small tumor tissue, and achieve the effect of improving anti-tumor effect in vivo, strong cytotoxicity, and low cytotoxicity

Active Publication Date: 2021-06-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drug delivery to residual tumor lesions or circulating tumor cells after surgery cannot be effectively accomplished by ordinary nano-drug delivery systems.
Because once most tumors are surgically eradicated, the remaining tumor tissue (<0.1g) will certainly be too small to produce an EPR effect

Method used

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  • A kind of biomimetic nano preparation based on platelet membrane fragments and its preparation method and application
  • A kind of biomimetic nano preparation based on platelet membrane fragments and its preparation method and application
  • A kind of biomimetic nano preparation based on platelet membrane fragments and its preparation method and application

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preparation example Construction

[0058] In yet another specific embodiment of the present invention, the preparation method of the above-mentioned biomimetic nano-preparation is provided, including:

[0059] The Cdk5 inhibitor and the chemotherapeutic drug are respectively dissolved, mixed evenly, added into the aqueous solution containing platelet membrane fragments, stirred for treatment, and obtained after dialysis.

[0060] In yet another specific embodiment of the present invention, the solvent selected for dissolving is dimethyl sulfoxide, which has a better dissolving effect; at the same time, in order to make the Cdk5 inhibitor and chemotherapeutics mix more uniformly, it is preferable to use an ultrasonic method to improve both. uniformity of mixing.

[0061] In yet another specific embodiment of the present invention, the addition is carried out in a dropwise manner; thereby making the antitumor drug components more uniformly dispersed;

[0062] Stirring treatment time is 10-40min;

[0063] Dialys...

Embodiment 1

[0093] Example 1: Preparation of Platelet Membrane Fragments

[0094] 1) Extraction of peripheral blood from mice: After fixing the mice, the eyeballs were picked up with tweezers and removed quickly, and the blood flowed from the orbits into EP tubes rinsed with EDTA.

[0095] 2) Platelet extraction: the collected whole blood sample was centrifuged at 100 g for 20 min at room temperature to separate and remove red blood cells and white blood cells to obtain platelet-rich plasma. The centrifugation was repeated once more under the same conditions for purification. Add EDTA and prostaglandin E1 to the purified platelet-rich plasma, and centrifuge at 800 g for 20 min at room temperature to obtain platelet pellets. Platelet pellets were redispersed in PBS containing EDTA and protease inhibitor tablets (Roche) and stored frozen.

[0096] 3) Preparation of platelet cell membrane fragments: the platelet cell membrane was physically destroyed by probe ultrasound, the power amplitude ...

Embodiment 2

[0097] Embodiment 2: Transmission Electron Microscopy (TEM) Identifying the Nanostructure of Platelet Membrane Fragments

[0098] Use a pipette gun to absorb 20 μL of platelet membrane fragment solution, carbon film copper grid, filter paper to absorb excess liquid, dry at room temperature, and place it under a transmission electron microscope for observation. The result is as figure 1 As shown in the transmission electron microscope pictures, it can be confirmed that the size of the newly prepared platelet membrane fragments is ~20nm, and the size is uniform and the dispersion is good.

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Abstract

The invention provides a bionic nano-preparation based on platelet membrane fragments and a preparation method and application thereof, which belong to the technical field of medicine. In the present invention, the cyclin-dependent kinase 5 (Cdk5) inhibitor and chemotherapeutic drugs are used in combination. On the one hand, the tumor cells are killed by chemotherapy, the immune system is activated to restore the IFN-γ content, and the immunosuppression caused by postoperative stress is alleviated. , on the other hand, down-regulate PD‑L1 through Cdk5 inhibitors, relieve IFN‑γ-related immune resistance, the combination of the two changes the duality of IFN‑γ, relieves its downstream immunosuppressive effect, and enables its positive effect At the same time, the biological tendency of platelets is used to solve the problem of targeted drug delivery after surgery, and through the improvement of the preparation method, the preparation method of biomimetic nanoparticles based on platelet membrane fragments is simple and the drug loading efficiency is high, so it has good practical application potential. value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a bionic nano-preparation based on platelet membrane fragments, a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Surgical resection is currently the first-line option for cancer treatment, but residual tumor cells are unavoidable after surgery, and some cells will dissociate into the peripheral blood circulation to form circulating tumor cells, resulting in postoperative recurrence and metastasis in a large number of patients. More and more evidence shows that the stress of tumor surgery destroys the host immune system,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K9/51A61K47/46A61K31/52A61K31/704A61P35/00
CPCA61K9/5176A61K31/52A61K31/704A61K45/06A61P35/00A61K2300/00
Inventor 栾玉霞李倩
Owner SHANDONG UNIV
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