Compound preparation for improving quality of upper tobacco leaves of flue-cured tobacco and preparation method and application of compound preparation

A compound preparation and tobacco leaf technology, applied in the field of tobacco production, can solve the problems of scattered application results, lack of technical level, strong pertinence, etc.

Active Publication Date: 2020-10-20
CHONGQING CHINA TOBACCO IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of the above directions have made research progress that can improve the quality of the upper tobacco leaves, but the application results are relatively scattered, highly targeted and lack of technical integration. There are certain limitations in the production of tobacco leaves, and it is difficult to deal with the determination of tobacco leaf raw materials. Quality Variety, Origin and Annual Differentiation Fluctuation

Method used

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  • Compound preparation for improving quality of upper tobacco leaves of flue-cured tobacco and preparation method and application of compound preparation
  • Compound preparation for improving quality of upper tobacco leaves of flue-cured tobacco and preparation method and application of compound preparation
  • Compound preparation for improving quality of upper tobacco leaves of flue-cured tobacco and preparation method and application of compound preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Compound preparation preparation: including the following steps,

[0036] S1: Activation culture of bacterial strains. The Bacillus cereus 4-a and Bacillus cereus 4-d obtained by screening were respectively activated and cultured on the activation plate medium.

[0037]Specifically, in a sterile working environment, use a sterilized spatula to scrape a small amount of bacteria from the slant culture medium in a closed test tube of the preserved strain, and apply it to an activated plate culture in a sterile petri dish with a three-section line separation method. On the base, grow to form a single colony. The formula of the activated plate medium is: 0.3% beef extract, 0.5% tryptone, 1% sodium chloride, 1.5% agar powder, adjust the pH value to 7.2 after making up the volume with distilled water, and heat it under high pressure at 121 ℃ Sterilize for 10 minutes, pour into a sterile petri dish to solidify.

[0038] S2: Expansion culture of bacterial strains, the single co...

Embodiment 2

[0060] The difference from Example 1 is that during the preparation of the compound preparation, tryptone is not contained in the physiological saline of the resuspended bacteria of the inoculum 1 and the inoculum 2; The volume ratio of body, enzyme preparation solution, and inorganic salt solution auxiliary agent is 1.2:10:38:15; during the baking process, on the basis of the local "three-stage six-step" flue-cured tobacco baking process in Pengshui, Chongqing, the yellowing period The initial temperature was lowered by 4°C, and the yellowing period was prolonged for 12 hours. The number of the treated sample added with the compound preparation working solution is B2, and the number of the blank control sample added with the same amount of water is C2. See Table 5 for the grade structure of the cured tobacco leaf samples, Table 6 for the sensory evaluation results of the sample cigarettes, and Table 7 and Table 8 for the routine chemical composition test results and chemical ...

Embodiment 3

[0074] The difference from Example 1 is that during the preparation of the compound preparation, before the bacteria were collected by centrifugation, the counts of live bacteria in the bacterial suspension of the two bacterial strains all reached 10. 9 pcs / ml; when preparing the stock solution of the compound preparation, the volume ratio of the first inoculum, the second inoculum, the enzyme preparation solution, and the inorganic salt solution auxiliary agent is 1.1:12:35:15; the inorganic salt solution used as the auxiliary agent at the same time The concentration is 0.05%; the neutral protease that makes up the enzyme preparation: α-amylase: cellulase: pectinase: β-glucanase=9:52:1.2:17:10; during the baking process, in Chongqing Based on the local "three-stage and six-step" flue-cured tobacco curing process in Pengshui, the initial temperature of the yellowing period is reduced by 2°C, and the temperature stabilization period of the yellowing period is extended to 18 hour...

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Abstract

The invention belongs to the technical field of tobacco production, and particularly discloses a compound preparation for improving the quality of upper tobacco leaves of flue-cured tobacco and a preparation method and application of compound preparation. The compound preparation comprises a first inoculum containing bacillus cereus 4-a, a second inoculum containing bacillus cereus 4-d and an enzyme preparation, the preservation number of the bacillus cereus 4-a is CGMCC No.3864, and the preservation number of the bacillus cereus 4-d is CGMCC No.5977. By adopting the upper tobacco leaves processed through the scheme, compared with contrast tobacco leaves which do not adopt the technology, the upper tobacco leaves of the flue-cured tobacco treated by adopting the technical scheme have the advantages that more efficient conversion of macromolecular substances in the curing process is facilitated, and the grade structure quality, sensory evaluation score, chemical component coordination and the like of the tobacco leaves are remarkably improved.

Description

technical field [0001] The invention relates to the technical field of tobacco production, in particular to a compound preparation for improving the quality of upper tobacco leaves of flue-cured tobacco, a preparation method and application thereof. Background technique [0002] The quality of tobacco leaf raw materials directly determines the quality and market sales of cigarette industry products, and its industrial availability greatly affects the tax and profit level of the tobacco industry. A large backlog of low-grade tobacco leaf raw materials has seriously occupied the funds of cigarette industry enterprises, and it is urgent to improve their utilization rate through new technological means and accelerate inventory digestion. The upper tobacco leaves of flue-cured tobacco in my country generally have defects such as insufficient maturity, unbalanced chemical composition, especially high content of nitrogen-containing compounds (such as nicotine, total protein), etc.,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20C12R1/125
CPCA24B15/20C12R2001/125C12N1/205Y02W30/40
Inventor 杜红毅孙兰茜汪长国王鹏黎洪利陈昆燕
Owner CHONGQING CHINA TOBACCO IND CO LTD
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