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Method for enhancing hydrogel through microbial mineralization

A hydrogel and microbial technology, applied in the field of microbial mineralization to enhance hydrogel, can solve the problems of poor tensile strength effect, negative impact on strength, and high requirements for initiators, achieve good economic and social benefits, simple preparation process, Broaden the effect of application scenarios

Active Publication Date: 2020-10-16
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The double network generally combines a rigid network with a flexible network to give the gel a certain self-healing performance, but it has higher requirements for the first network. Now this disadvantage has been improved through research, but most of the chemical binders used have Toxicity, reducing the biocompatibility of the matrix; nanocomposites are generally organic-inorganic composites, and special properties can be endowed to the gel by changing the type of composite nanoparticles, such as: Ag + Antibacterial, but such inorganic nanoparticles are prone to agglomeration in the gel, which will have a negative impact on the strength, and the requirements for the initiator are relatively high; the macromolecular microsphere gel can well achieve the effect of stress dispersion, so Commonly used to increase the compressive strength, but the corresponding effect on the tensile strength is relatively poor

Method used

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  • Method for enhancing hydrogel through microbial mineralization
  • Method for enhancing hydrogel through microbial mineralization
  • Method for enhancing hydrogel through microbial mineralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 (blank group gel 1)

[0029] A method for hydrogel comprising the steps of:

[0030] (1) 1.8g of polyvinyl alcohol (PVA) particles were stirred in a high temperature (90-95°C) water bath for 2 hours and completely dissolved in deionized water to make a uniform and transparent PVA solution, and then 1.8g of urea and 3.33g of calcium chloride were dissolved in In the PVA solution, cool to room temperature to obtain a mixed solution, wherein the concentration of PVA is 6wt%, and the concentrations of urea and calcium chloride are 1M;

[0031] (2) Vacuum filter the mixed solution at 0.1MPa for 7min and 3 times, remove the air bubbles in it, and then injection mold it (the size of the mold is 90mm×10mm×3mm), then freeze at -18°C for 20h, then thaw at room temperature for 4h, alternately Cycle 3 times to obtain the blank gel.

[0032] The obtained blank group gel 1 was reacted at room temperature for 7 days, and the tensile strength of the hydrogel was tested b...

Embodiment 2

[0034] A method for microbial mineralization enhanced hydrogel, comprising the following steps:

[0035] (1) The medium used for the culture of urease bacteria is 0.13M Tris buffer, 10g / L (NH 4 ) 2 SO 4 , 20g / L yeast extract, sterilized at 120°C for 25min, then cooled to room temperature, inoculated with 1% volume of the original bacterial liquid, cultured on a shaker at 200rpm, 30°C for 24h to obtain mature bacterial liquid; then centrifuged at 8000rpm for 12min to obtain concentrated bacteria solution, the concentration of the bacterial solution was adjusted to 5×10 9 cell / mL, OD 600 The value is 1.9;

[0036] (2) 1.8g polyvinyl alcohol (PVA) particles were stirred in a high temperature (90-95°C) water bath for 3 hours and completely dissolved in deionized water to make a uniform and transparent PVA solution, and then 1.8g urea and 3.33g calcium chloride were dissolved in In the PVA solution, cool to room temperature, add 3mL of concentrated bacterial solution and stir ...

Embodiment 3

[0040] Compared with Example 2, only the dosage of the bacterial solution was changed to 5%, that is, the dosage of the concentrated bacterial solution was 1.5 mL. The prepared hydrogel was reacted at room temperature for 7 days, and the tensile strength of the hydrogel was tested by a universal testing machine. The loading speed was 100 mm / min, and the tensile strength of the sample was 0.51 MPa.

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Abstract

The invention discloses a method for enhancing hydrogel through microbial mineralization. The method comprises the following steps: 1) culturing urease bacteria liquid and preparing concentrated bacteria liquid; 2) preparing a uniform and transparent gel matrix solution, then adding urea and calcium chloride, uniformly stirring, adding the concentrated bacteria solution at room temperature, and uniformly stirring to obtain a mixed solution; and 3) carrying out vacuum filtration on the mixed solution to remove bubbles, molding by using a mold, and carrying out freeze-thaw circulation to obtainthe microbial mineralization enhanced hydrogel. According to the method, a calcium carbonate network structure can be generated in the gel through microbial mineralization, thereby achieving the purpose of gel enhancement.

Description

technical field [0001] The invention relates to a method for microbial mineralization enhancing hydrogel, which belongs to the field of hydrogel preparation. Background technique [0002] Hydrogels are hydrophilic materials with a three-dimensional network structure. With a certain shape and soft properties, it can absorb a large amount of water. Due to its good biocompatibility, hydrogels were originally widely used in the field of medicine, such as: drug delivery and release, tissue engineering, etc.; now, due to its high water absorption High water retention, its application has already expanded to many fields such as daily necessities, industrial and agricultural water retention and dust removal. Correspondingly, the gel substrates used in different fields are also different, and can be roughly divided into two categories: natural and synthetic. Among them, natural gel has good biocompatibility and low price, but has poor stability and is easy to degrade; The structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L29/04C08L89/00C08K5/21C08K3/16
CPCC08J3/075C08J2329/04C08J2489/00C08K5/21C08K2003/162
Inventor 王瑞兴张思逸章炜徐刚
Owner SOUTHEAST UNIV
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