Preparation method and application of ginsenoside composition
A technology of ginsenoside and composition, which is applied in the field of preparation of ginsenoside composition, can solve the problems of low yield of rare ginsenoside and the like, and achieve the effect of strong repairing ability
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Embodiment 1
[0038] Embodiment 1 Pichia pastoris transforms rare ginsenosides
[0039] 1. Preparation of 1% ginseng whole plant extract
[0040] According to the ratio (weight ratio) of roots: stems and leaves: flowers: seeds = 90:5:2.5:2.5, take 200g of ginseng whole plant samples, crush them to about 20 mesh with a pulverizer, add 2000mL of deionized water, and heat in a water bath at 96°C 2h, filter and centrifuge to obtain the filtrate; add 2000mL deionized water to the filter residue, heat in water bath for 2h, filter and centrifuge to obtain the filtrate; % Panax ginseng whole plant extract freeze-dried powder.
[0041] 2. Experiment of transformation of ginsenoside by Pichia pastoris
[0042] Using potato dextrose broth (1L medium containing 0.6% potato extract, 2.0% glucose, pH 5.6±0.2) as the seed medium, pick the bacterial liquid in the -80°C preservation tube of SP-02-DZ-08, Culture in a shaker at 30°C, 200rpm shaking, and obtain preliminary seed liquid after 16-18 hours. Acco...
Embodiment 2
[0050] Example 2 Cell scratch repair of Pichia pastoris ginseng fermentation product
[0051] To study the effect of 1% ginseng whole plant extract fermentation product of aerospace yeast SP02-DZ-08 on scratch injury of human keratinocytes.
[0052] Table 2 Samples and concentrations to be tested
[0053] Sample name full ingredients Final concentration 1% Ginseng Extract 1% Ginseng Extract 1%,0.05% Fermentation product of 1% ginseng whole plant extract of SP02-DZ-08 1% Ginseng Extract Fermentation Broth 1%,0.05%
[0054] 1) Cell culture: Keratinocytes (AL17003FK P2), seeded in 6-well plates at 200,000 / well, 5% CO at 37°C 2 Cultured in a cell culture incubator for 3 days.
[0055] 2) Sample treatment: After the solution was filtered and sterilized before and after fermentation, it was diluted 100 and 200 times with medium, and the final test concentration was 1%. Cells were incubated at 37°C, 5% CO in medium containing the sample to be test...
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