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Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation

A technology of denitrifying bacteria and composite microecology, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as increased cost, total nitrogen accumulation, single cultivation, etc., and save time and cost , full absorption, and the effect of improving fermentation efficiency

Inactive Publication Date: 2020-10-13
KESHENG ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are related invention patents for the cultivation of nitrifying bacteria, but it only cultivates a single nitrifying bacteria preparation. A single strain will cause the accumulation of total nitrogen when treating sewage, so it is necessary to cooperate with denitrifying bacteria to denitrify
However, at present, the cultivation of nitrifying bacteria and denitrifying bacteria comes from separate cultivation, or mixing after cultivation to obtain compound microbial preparations. This process is time-consuming and increases costs.

Method used

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  • Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation
  • Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation
  • Combined fermentation method of nitrifying-denitrifying bacteria composite microecological preparation

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Effect test

Embodiment 1

[0035] A combined fermentation method of nitrifying-denitrifying bacteria compound probiotics, specifically comprising the following steps:

[0036] S1, the preparation of bacterial strain, obtain Nitrobacter victoria from aerobic aeration tank by laboratory separation, obtain denitrifying bacteria from river bottom mud separation;

[0037] S2, the preparation of culture medium, specifically as follows:

[0038] Denitrifying bacteria medium:

[0039] yeast powder 2g / l,

[0040] Sodium acetate 2.5g / l,

[0041] Dipotassium hydrogen phosphate 0.5g / l,

[0042] Magnesium sulfate 0.25g / l, the culture medium was sterilized at 121°C for 20 minutes, set aside;

[0043] Nitrobacter culture medium:

[0044] Sodium chloride 1g / l,

[0045] Sodium nitrite 0.5g / l,

[0046] Anhydrous sodium acetate 3g / l,

[0047] Dipotassium hydrogen phosphate 3g / l,

[0048] Manganese sulfate 0.2g / l,

[0049] Magnesium sulfate 0.2g / l,

[0050] Ferrous sulfate 0.1%;

[0051] S3, get 1000L of the ab...

Embodiment 2

[0055] A combined fermentation method of nitrifying-denitrifying bacteria compound probiotics, the combined fermentation method specifically includes the following steps:

[0056] S1, the preparation of bacterial strain, obtain Nitrobacter victoria from aerobic aeration tank by laboratory separation, obtain denitrifying bacteria from river bottom mud separation;

[0057] S2, the preparation of culture medium, specifically as follows:

[0058] Denitrifying bacteria medium:

[0059] yeast powder 4g / l,

[0060] Sodium acetate 5g / l,

[0061] Dipotassium hydrogen phosphate 1g / l,

[0062] Magnesium sulfate 0.25g / l, the culture medium was sterilized at 121°C for 20 minutes, set aside;

[0063] Nitrobacter culture medium:

[0064] Sodium chloride 1g / l,

[0065] Sodium nitrite 0.5g / l,

[0066] Anhydrous sodium acetate 3g / l,

[0067] Dipotassium hydrogen phosphate 3g / l,

[0068] Manganese sulfate 0.2g / l,

[0069] Magnesium sulfate 0.2g / l,

[0070]Ferrous sulfate 0.1%;

[007...

Embodiment 3

[0075] The invention also provides the engineering application of the nitrifying-denitrifying bacterial fermentation liquid.

[0076] The fermented liquid of the above-mentioned joint fermentation was applied to the domestic sewage treatment pumping station in Liuhe District, Nanjing City, and the pumping station treated 500 cubic meters of sewage per day. Add the compound microbial preparation of the present invention into the aerobic tank and the denitrification tank, the dosage ratio is 5:1; the initial dosage is 100L, 50L is added on the 4th day, and 50L is added on the 8th day, and the water quality is monitored in real time Change, record the content of nitrite and nitrate, the results are shown below.

[0077] Effect of Nitrification-denitrification Bacteria on Treatment of Total Nitrogen in Domestic Sewage

[0078]

[0079] Control group (without adding bacteria):

[0080]

[0081] It can be concluded from the table that after adding the compound microbial prep...

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Abstract

The invention relates to the field of biotechnology, in particular to a combined fermentation method of a nitrifying-denitrifying bacteria composite microecological preparation. The composite microecological preparation consists of Nitrobacter winogradsky KS05 and denitrifying bacteria KS03. The combined fermentation method specifically includes the following steps: preparing a strain; preparing culture media including a denitrifying bacteria culture medium and a Nitrobacter culture medium; inoculating the Nitrobacter culture medium with the Nitrobacter winogradsky KS05 while maintaining the concentration of sodium nitrite; and after culture of the nitrifying bacteria, adding the denitrifying bacteria culture medium on the basis, inoculating the denitrifying bacteria KS03, and when the content of nitrite and nitrate in the culture medium reaches 0 mg / l, ending the mixed culture. The staged continuous cultivation of nitrifying bacteria and denitrifying bacteria of the present inventioncan complete the nitrification and denitrification culture enrichment problems in a short time, save time and cost, improve fermentation efficiency, and reduce the cost in actual production.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a joint fermentation method of a nitrifying-denitrifying bacterial compound microecological preparation. Background technique [0002] Today, due to the impact of human activities, the ammonia nitrogen indicators in urban rivers and relatively open waters remain high. The resulting ecological and environmental problems have had a major impact on the human home environment and the image of the city. Microbial restoration of the water environment has become a hot research field at present. At present, the removal of ammonia nitrogen, nitrite, and nitrate in sewage water is gradually being upgraded to a new level along with the research on nitrifying bacteria and denitrifying bacteria. [0003] There are organic nitrogen, ammonia nitrogen, nitrate nitrogen and other forms of nitrogen in wastewater, of which ammonia nitrogen and organic nitrogen are the main forms. In the proce...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/01
CPCC02F3/02C02F3/34C12N1/20Y02W10/10
Inventor 王凯朱辉
Owner KESHENG ENVIRONMENTAL PROTECTION TECH CO LTD
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