Sodium dodecyl sulfate-bacitracin compound and composite oil displacement agent
A technology of sodium dodecyl sulfonate and compound oil displacement, which is applied in the directions of bacitracin, drilling compositions, peptides, etc., can solve the problem that microbial oil displacement cannot be realized, reduce the effect of microbial oil displacement, and destroy the physiological functions of microorganisms, etc. problem, to achieve the effect of improving the efficiency of synergistic reaction, strong operability and applicability, and expanding the swept volume
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Embodiment 1
[0070] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0071] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0072] (2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain bacitracin (during the chromatographic separation, the retention time of the upper capsule solution was based on the bacitracin stand...
Embodiment 2
[0079] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0080] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0081] (2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain bacitracin (during the chromatographic separation, the retention time of the upper capsule solution was based on the bacitracin stand...
Embodiment 3
[0085] This embodiment provides a kind of microbial-chemical composite oil displacement agent, and it is prepared through the following steps:
[0086] (1) Add 200mL of the culture medium of the inoculated strain to a 500mL Erlenmeyer flask, place it in an air-bath shaker at 65°C and 170r / min for cultivation, and when the bacteria grow to the late logarithmic period, centrifuge at 8000r / min for 15min to collect the cells , washed with dilute sulfuric acid (pH value = 2) to remove impurities, and suspend the bacteria to make a suspension (1cm cuvette) with an absorbance value of 1 at a wavelength of 600nm. Centrifuge for 10 minutes to collect the lower layer of bacteria;
[0087] (2) Bacteria were eluted with water at 40°C for 12 hours, centrifuged, and the upper capsule solution was taken for chromatographic separation and purification to obtain bacitracin (during the chromatographic separation, the retention time of the upper capsule solution was based on the bacitracin stand...
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