Industrial in-vitro procambarus clarkii breeding method
A technology of Crayfish and isolated seedlings is applied in the direction of climate change adaptation, fish farming, application, etc. It can solve the problem that it cannot adapt to large-scale specialized production, restricts the benign and sustainable development of the industry, and can only be suitable for small-scale production. In situ breeding and other issues, to achieve the effect of excellent facilities, high production efficiency of seedlings, and promotion of upgrading and development
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Embodiment 1
[0018] A method for industrialized isolation of Procambarus clarkii seedlings, including the acquisition of brood shrimp (centralized bulk purchase or cultivation in special ponds), selection and classification of brood shrimp, in vitro operation of fertilized egg embryos, hatching and development of fertilized egg embryos, and circulating water quality Process control.
[0019] The parental shrimp mating and spawning pool (greenhouse or open-air earthen pool) has an area of 5-10 mu, a pool depth of 1.5-1.8m, and a water depth of 0.6-1m. There are 50-100 artificial shrimp nests for Procambarus clarkii in the pool .
[0020] The specification range of parental shrimps used for cultivating egg-bearing shrimps is 20-30g for female shrimps and 25-35g for male shrimps.
[0021] The ovum-bearing shrimps are divided into three categories according to the development degree of fertilized egg embryos: the first category is unruptured membranes, and the color of fertilized egg embryo...
Embodiment 2
[0025] The area of the open-air broodstock mating and spawning pool is 10 mu, the pool depth is 1.8m, and the water depth is 0.8-1m. There are 50-100 special artificial shrimp nests / mu in the pool. The size range of the parental shrimp used for cultivating egg-bearing shrimp is 20-30g for female shrimp, 25-35g for male shrimp, and the ratio of male to female is 2-3:1.
[0026] According to the degree of development of fertilized egg embryos, egg-bearing shrimps are divided into three categories: the first category is unruptured membranes, and the color of fertilized egg embryos is gray and translucent and will soon rupture the membranes; the second category is unruptured membranes, and the color of fertilized egg embryos is black Or brown, it takes a long time for the embryo to develop; the third category is that the fertilized egg in the abdomen has ruptured the membrane.
[0027] The fertilized eggs of the first type of brood shrimp are operated in vitro, and artificial or...
Embodiment 3
[0030] The greenhouse is used as a mating and spawning pool, with an area of 5 mu (conjoined greenhouse), a pool depth of 1.5m, and a water depth of 0.8m. There are 50-100 special artificial shrimp nests / mu in the pool. The size range of the parental shrimp used for cultivating egg-bearing shrimp is 20-30g for female shrimp, 25-35g for male shrimp, and the ratio of male to female is 2-3:1.
[0031] According to the degree of development of fertilized egg embryos, egg-bearing shrimps are divided into three categories: the first category is unruptured membranes, and the color of fertilized egg embryos is gray and translucent (the membranes will soon be ruptured); the second category is unruptured membranes, and the color of fertilized egg embryos is It is black or brown (it takes a long time for the embryo to develop); the third type is that the fertilized egg in the abdomen has ruptured the membrane.
[0032] The fertilized eggs of the first type of brood shrimp are operated ...
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