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Industrial in-vitro procambarus clarkii breeding method

A technology of Crayfish and isolated seedlings is applied in the direction of climate change adaptation, fish farming, application, etc. It can solve the problem that it cannot adapt to large-scale specialized production, restricts the benign and sustainable development of the industry, and can only be suitable for small-scale production. In situ breeding and other issues, to achieve the effect of excellent facilities, high production efficiency of seedlings, and promotion of upgrading and development

Inactive Publication Date: 2020-10-13
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the seasonal centralized listing of finished shrimp in the conventional farming mode also greatly restricts the further sustainable development of the industry
[0003] At present, the seedlings of Procambarus clarkii mainly come from paddy fields and ponds by self-propagation and self-breeding, or by purchasing shrimp seedlings for stocking. Because the shrimp has a small amount of eggs, a high rate of self-mutilation, and a low survival rate of seedlings, this conventional breeding The output and efficiency of the method are low, and the benefit is poor. For example, the yield per mu of rice fields and ponds is only tens of thousands, and the output is only about two to three hundred catties. It can only be suitable for small-scale local breeding, and cannot adapt to large-scale professional production , and Procambarus clarkii has no industrialized specialized nursery farms or fine seed farms like river crabs and Macrobrachium rosenbergii. The technical model of industrialized and large-scale isolated seedlings is still blank. The key link that urgently needs to be solved for the further sustainable development of the Procambarus suberi aquaculture industry
[0004] In addition, in terms of finished shrimps, due to the obvious seasonality of Procambarus clarkii farming, the main breeding period is generally from April to May every year, feeding for 45-60 days to catching and selling from May to July. The model leads to the seasonal concentration of finished shrimps on the market, and the market price drops sharply, which directly affects the breeding benefits. However, in the spring, autumn and winter of the year (November to April of the following year), the price of finished shrimps rises sharply due to the scarcity of finished shrimps, that is, When there are shrimps in the market, the price is too low, but when there is market, there is no supply of finished shrimps in the market. This situation greatly restricts the further healthy and sustainable development of the industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for industrialized isolation of Procambarus clarkii seedlings, including the acquisition of brood shrimp (centralized bulk purchase or cultivation in special ponds), selection and classification of brood shrimp, in vitro operation of fertilized egg embryos, hatching and development of fertilized egg embryos, and circulating water quality Process control.

[0019] The parental shrimp mating and spawning pool (greenhouse or open-air earthen pool) has an area of ​​5-10 mu, a pool depth of 1.5-1.8m, and a water depth of 0.6-1m. There are 50-100 artificial shrimp nests for Procambarus clarkii in the pool .

[0020] The specification range of parental shrimps used for cultivating egg-bearing shrimps is 20-30g for female shrimps and 25-35g for male shrimps.

[0021] The ovum-bearing shrimps are divided into three categories according to the development degree of fertilized egg embryos: the first category is unruptured membranes, and the color of fertilized egg embryo...

Embodiment 2

[0025] The area of ​​the open-air broodstock mating and spawning pool is 10 mu, the pool depth is 1.8m, and the water depth is 0.8-1m. There are 50-100 special artificial shrimp nests / mu in the pool. The size range of the parental shrimp used for cultivating egg-bearing shrimp is 20-30g for female shrimp, 25-35g for male shrimp, and the ratio of male to female is 2-3:1.

[0026] According to the degree of development of fertilized egg embryos, egg-bearing shrimps are divided into three categories: the first category is unruptured membranes, and the color of fertilized egg embryos is gray and translucent and will soon rupture the membranes; the second category is unruptured membranes, and the color of fertilized egg embryos is black Or brown, it takes a long time for the embryo to develop; the third category is that the fertilized egg in the abdomen has ruptured the membrane.

[0027] The fertilized eggs of the first type of brood shrimp are operated in vitro, and artificial or...

Embodiment 3

[0030] The greenhouse is used as a mating and spawning pool, with an area of ​​5 mu (conjoined greenhouse), a pool depth of 1.5m, and a water depth of 0.8m. There are 50-100 special artificial shrimp nests / mu in the pool. The size range of the parental shrimp used for cultivating egg-bearing shrimp is 20-30g for female shrimp, 25-35g for male shrimp, and the ratio of male to female is 2-3:1.

[0031] According to the degree of development of fertilized egg embryos, egg-bearing shrimps are divided into three categories: the first category is unruptured membranes, and the color of fertilized egg embryos is gray and translucent (the membranes will soon be ruptured); the second category is unruptured membranes, and the color of fertilized egg embryos is It is black or brown (it takes a long time for the embryo to develop); the third type is that the fertilized egg in the abdomen has ruptured the membrane.

[0032] The fertilized eggs of the first type of brood shrimp are operated ...

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PUM

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Abstract

The invention relates to an industrial in-vitro procambarus clarkii breeding method, and belongs to the technical field of freshwater economic shrimp fry breeding. The method comprises the following steps of performing selective purchasing or cultivating in a special pond to obtain berried shrimps, selecting and classifying the collected berried shrimps, then carrying out fertilized egg embryo in-vitro operation, and finally carrying out centralized artificial hatching on obtained in-vitro fertilized egg embryos. The treatment control of the circulating water quality is ensured in the hatchingprocess. According to the method, indoor controllable industrial large-scale breeding is performed on fertilized eggs of procambarus clarkii through in-vitro hatching, so that the production efficiency of artificial breeding is remarkably improved, the controllability of indoor artificial large-scale breeding of the procambarus clarkii and the planning property of production are realized, the artificial breeding and forming mode of the procambarus clarkii is innovated, and the upgrading development of procambarus clarkii fry breeding and breeding modes is effectively promoted.

Description

technical field [0001] The invention relates to a method for industrialized isolated seedlings of Procambarus clarkii, in particular to factory-scale artificial breeding of Procambarus clarkii, and belongs to the technical field of seed breeding of freshwater economic shrimps. Background technique [0002] Procambarus clarkii, commonly known as freshwater crayfish, belongs to Arthropoda, Crustacea, Crustacea, Subclass, Decapod, Cricketidae. It is native to North America. It was introduced to my country from Japan in 1929. It has been widely distributed in rivers, ponds and major water systems in my country. Among them, it has become an important economic species for freshwater aquaculture and comprehensive rice-fishery breeding in my country. With the increasing market consumption and export volume of Procambarus clarkii, artificial breeding, especially the rice shrimp breeding mode, has shown a blowout growth in recent years, and the scale and output of farming are expanding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 徐增洪许洪杰陈浩王根林刘国锋水燕
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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