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Separation method of phenolic acid glucoside compounds in juglans regial L. mature seed diaphragma juglandis fructus and application

A technology of walnut distraction wood and glucoside, which is applied in chemical instruments and methods, medical preparations containing active ingredients, applications, etc., and can solve problems such as unclear biological activity functions and the like

Active Publication Date: 2020-09-25
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, which specific compounds and their corresponding biological activity functions are not yet clear, and further in-depth research is needed to confirm

Method used

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  • Separation method of phenolic acid glucoside compounds in juglans regial L. mature seed diaphragma juglandis fructus and application
  • Separation method of phenolic acid glucoside compounds in juglans regial L. mature seed diaphragma juglandis fructus and application
  • Separation method of phenolic acid glucoside compounds in juglans regial L. mature seed diaphragma juglandis fructus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Obtain the extraction method of these three kinds of phenolic acid glucoside compounds from walnut, specifically as follows:

[0046] After walnut split wood was crushed, 75% ethanol was added according to the ratio of material to liquid 1:15 (M / V), stirred and extracted at 50°C for 3 hours, and the supernatant was collected by filtration, and this was repeated 3 times. The supernatant obtained three times was concentrated under reduced pressure to remove ethanol to obtain walnut alcohol extract. Put the walnut alcohol extract on the MCI column, and sequentially elute with deionized water, 30%, 50%, and 70% ethanol, collect the water elution and 30% ethanol elution parts, combine them, concentrate under reduced pressure and load the sample on The MCI column was sequentially eluted with deionized water, ethanol with a volume fraction of 10%, 20%, 30%, and 50%, and the eluted part of 10% ethanol was collected, concentrated under reduced pressure, and then loaded onto the ...

Embodiment 2

[0048] Obtain the extraction method of these three compounds from walnut, specifically as follows:

[0049]After the walnut heartwood is crushed, add 75% ethanol according to the ratio of material to liquid 1:10 (M / V), stir and extract at 40°C for 5 hours, filter to get the supernatant, and repeat this 3 times. The supernatant obtained three times was concentrated under reduced pressure to remove ethanol to obtain walnut alcohol extract. Put the walnut alcohol extract on the MCI column, and sequentially elute with deionized water, 30%, 50%, and 70% ethanol, collect the water elution and 30% ethanol elution parts, combine them, concentrate under reduced pressure and load the sample on The MCI column was sequentially eluted with deionized water, ethanol with a volume fraction of 10%, 20%, 30%, and 50%, and the eluted part of 10% ethanol was collected, concentrated under reduced pressure, and then loaded onto the ODS column. Deionized water, 5%, 10%, and 20% ethanol solution by ...

Embodiment 3

[0051] Obtain the extraction method of these three compounds from walnut, specifically as follows:

[0052] After walnut split wood was crushed, 75% ethanol was added according to the ratio of material to liquid 1:20 (M / V), stirred and extracted at 70°C for 2 hours, and the supernatant was collected by filtration, and this was repeated 3 times. The supernatant obtained three times was concentrated under reduced pressure to remove ethanol to obtain walnut alcohol extract. Put the walnut alcohol extract on the MCI column, and sequentially elute with deionized water, 30%, 50%, and 70% ethanol, collect the water elution and 30% ethanol elution parts, combine them, concentrate under reduced pressure and load the sample on The MCI column was sequentially eluted with deionized water, ethanol with a volume fraction of 10%, 20%, 30%, and 50%, and the eluted part of 10% ethanol was collected, concentrated under reduced pressure, and then loaded onto the ODS column. Deionized water, 5%,...

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Abstract

The invention discloses a separation method of phenolic acid glucoside compounds in juglans regial L. mature seed diaphragma juglandis fructus and an application, and belongs to the technical field offoods. The method comprises the following steps of (1) performing extraction: crushing the juglans regial L. mature seed diaphragma juglandis fructus, and performing extraction with alcohol to obtainjuglans regial L. mature seed alcohol extracts; and (2) performing separation: performing column chromatography on the juglans regial L. mature seed alcohol extracts obtained in the step (1), sequentially performing eluting with ethanol of different concentrations, performing repeated purifying and eluting, and performing separation to obtain the phenolic acid glucoside compounds, wherein the phenolic acid glucoside compounds are 2-hydroxyl-5-methoxylphenyl-O-beta-D-glucopyranoside, 6-hydroxyl naphthyl-1,2- ( 2-O-beta-D-glucopyranoside) and 2,6-dihydroxy naphthyl -O-beta-D-glucopyranoside. Pharmacological experiments confirm that the three compounds have favorable antioxidant activity.

Description

technical field [0001] The invention relates to a separation method and application of phenolic acid glucoside compounds in walnut centipede, belonging to the technical field of food. Background technique [0002] Walnut is the mature seed of Juglans regial L., which is rich in unsaturated fatty acids. It is known as the "King of Woody Oils". It can improve cerebral ischemia, resist fatigue and improve memory. However, the current comprehensive utilization and development of walnut products is still not enough, such as the center wood in the walnut by-product. At present, the literature reports on walnut heartwood show that there are flavonoids such as quercetin and catechin in walnut heartwood, volatile substances such as menthol and cedrol, and phenolic compounds such as gallic acid, and have antibacterial, antibacterial, and antibacterial properties. Anti-oxidation, hypoglycemic, inhibition of brain function decline, anti-tumor and other active functions. However, which...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08A61K31/7034A61K31/704A61P39/06A61P11/00A61P29/00A61P17/00A61P3/10A61P25/00A61P35/00A23L33/105A61K8/60A61Q19/08
CPCC07H15/203C07H1/08A61K31/7034A61K31/704A61P39/06A61P11/00A61P29/00A61P17/00A61P3/10A61P25/00A61P35/00A23L33/105A61K8/602A61Q19/08A23V2002/00A23V2200/30A23V2250/21A61K2300/00A61K36/52A61K2236/15A61K2236/333A61K2236/39
Inventor 孙秀兰徐一达徐德平张银志耿树香宁德鲁马婷
Owner JIANGNAN UNIV
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