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High-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and application thereof

A technology of LP-08 and Lactobacillus plantarum is applied to Lactobacillus plantarum LP-08 and its application field to achieve the effects of reducing crude fiber content and changing nutritional components

Pending Publication Date: 2020-09-22
清远一生自然生物研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no related reports on the use of ARTP to mutate Lactobacillus plantarum and screen the mutant strains of Lactobacillus plantarum tolerant to high concentrations of tea dregs for tea dregs fermentation

Method used

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  • High-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and application thereof
  • High-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and application thereof
  • High-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The ARTP mutagenesis of embodiment 1 bacterial strain

[0056] 1. Strain activation

[0057] Inoculate the glycerol bacteria of Lactobacillus plantarum on the slant medium of the MRS medium, and incubate at 30°C for 24 hours. After the culture is over, take a ring of the strain from it and put it into a fresh slant medium, and incubate it at 30°C for 16 hours to further strengthen the viability of the strain. , rejuvenate the strain to achieve the purpose of strain activation.

[0058] 2. Determination of strain ARTP mutagenesis parameters

[0059] Add sterile physiological saline to the slant that has been activated and cultivated, elute, prepare a bacterial suspension, and control the OD of the bacterial suspension 600nm The value is between 0.5-0.7. Take 10 μL of the bacterial suspension and evenly spread it on the surface of the metal slide. After drying, use sterilized forceps to transfer the plate with the sample slide to the ARTP operating chamber. Use high-p...

Embodiment 2

[0064] The domestication screening of embodiment 2 mutant strains

[0065] Take the ARTP-treated bacterial suspension and inoculate it directly into the acclimatization medium, the liquid volume is 50mL of 250mL triangular flask, 30°C, 220rpm, culture for 72h, and investigate the growth of the strain in the medium with tea residue as the main carbon source and reproductive capacity. And by gradually increasing the content of tea residue in the acclimation medium, combined with plate separation, an excellent bacterial strain with strong growth ability, tolerance and utilization of high-concentration tea residue is obtained, preserved, and used for future use. details as follows:

[0066] (1) Domestication of mutant strains by low concentration of tea dregs

[0067] The ARTP-treated bacterial suspension was directly transferred to the acclimatization medium for cultivation, and the untreated strain was used as a control to observe the color and odor changes of the fermentation...

Embodiment 3

[0077] The identification of embodiment 3 Lactobacillus plantarum LP-08

[0078] 1. Morphological characteristics

[0079] The Lactobacillus plantarum LP-08 strain was prepared into a bacterial suspension and spread on the MRS medium after dilution. After culturing at 30°C for 48 hours, the colony and cell morphology were observed.

[0080] The results showed that after being cultured on the MRS medium for 48 hours, the surface diameter of the colony was about 3 mm, and it was round and convex, smooth and compact; the cells were rod-shaped by microscopic observation, single or in pairs or in short chains.

[0081] 2. Physiological and biochemical characteristics

[0082] According to the bacterial identification manual, the physiological and biochemical characteristics of Lactobacillus plantarum LP-08, such as VP reaction, carbon source utilization, and indole test, were determined.

[0083] The results showed that the strain was a Gram-positive bacterium without producing s...

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Abstract

The present invention relates to the technical field of microbial mutation breeding and particularly to a high-concentration tea dreg-tolerant lactobacillus plantarum LP-08 strain and an application thereof. The lactobacillus plantarum LP-08 is deposited in Guangdong Microbial Culture Collection Center (GDMCC) on June 16, 2020 and has a deposit number of GDMCC No:61061. The present invention aimsto develop ground-origin green tea dregs to prepare functional bacterial tea biological feeds, an ARTP mutagenesis and domestication screening method is used to select functional microorganisms, and the lactobacillus plantarum LP-08 that can tolerate high-concentration tea dregs and is good in biomass and metabolite activity is obtained. The strain can be used for tea dreg fermentation, can changenutrient ingredients in tea dregs, can be applied to development of the tea dreg feeds, and is of great significance for high-value utilization of tea dreg resources.

Description

technical field [0001] The invention relates to the technical field of microbial mutation breeding, in particular to a strain of Lactobacillus plantarum LP-08 tolerant to high-concentration tea dregs and its application. Background technique [0002] my country is a big tea-producing country. In 2018, the output of dry raw tea exceeded 2.6 million tons, of which green tea accounted for more than 65%. It is the main producer of green tea in the world, and its total output exceeds 75% of the total global green tea output. Green tea is a traditional beverage, and its edible part only accounts for a small part of the tea. At present, with the extension of the tea industry chain, more dry tea enters the tea deep-processing enterprises, and prepares instant tea powder and tea concentrate through high-temperature water extraction. The total amount of dry matter after deep processing of the current tea leaves is only about 3% of the dry weight of the tea leaves, and the content of a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C12N15/01A23K10/12A23K10/37C12R1/25
CPCC12N1/36C12N15/01A23K10/12A23K10/37C12R2001/25C12N1/205A23V2400/169Y02P60/87
Inventor 张文梁玲蒋顺进郑雪媚翁雪清赵颖
Owner 清远一生自然生物研究院有限公司
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