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Application of heme oxygenase-1 in diagnosis and treatment of radiation-induced lung injury

A technology of heme oxygenase and radiation-induced lung injury, which is applied in the application field of heme oxygenase-1 in the diagnosis and treatment of radiation-induced lung injury, and can solve problems such as unresearched effects

Inactive Publication Date: 2020-09-18
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found that HO-1 has a protective effect on various lung diseases such as acute lung injury induced by sepsis, bleomycin-induced pulmonary fibrosis, and chronic obstructive disease, but HO-1 has a protective effect on RILI induced by high-dose X-rays. There are no related researches and reports on the role and mechanism of

Method used

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  • Application of heme oxygenase-1 in diagnosis and treatment of radiation-induced lung injury
  • Application of heme oxygenase-1 in diagnosis and treatment of radiation-induced lung injury
  • Application of heme oxygenase-1 in diagnosis and treatment of radiation-induced lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Establishment of mouse RILI model

[0027] In order to be as consistent as possible with the RILI secondary to clinical thoracic tumor radiotherapy, we chose to irradiate the lungs of the mice: After the mice were anesthetized, they were fixed to the X-RAD SmART small animal radiotherapy simulation locator with biological tape Keep it in the prone position, with the upper limbs forward, the lower limbs backward, the limbs balanced and straight, and the CT positioning ( figure 1 A) Under the guidance, outline the scope of lung irradiation, try to avoid the heart and spine ( figure 1 B). After locating the lungs of the mice, X-ray was used to irradiate 13.5Gy with a single dose rate of 3Gy / min. After the irradiation, the mice were removed and returned to the cage for normal breeding. The materials were taken after 7 days and the analysis was carried out. Rat lung injury.

[0028] Experimental results:

[0029] Through the analysis of pathological H&E staining of m...

Embodiment 2

[0030] Example 2: Determination of HO-1 expression in lung tissue and lung macrophages of RILI mice

[0031] To investigate whether HO-1 is involved in the regulation of RILI, the total protein in the lung tissue of RILI mice was extracted, and the expression level of HO-1 was detected by Western blot.

[0032] Experimental results: X-ray can significantly induce the expression of HO-1 in lung tissue ( figure 2 A and 2C), suggesting that HO-1 is involved in X-ray-induced RILI. In addition, this study used to isolate RILI mouse lung macrophages and detect the expression of HO-1. The results showed that X-ray can induce the increase of HO-1 expression in lung macrophages ( figure 2 B and 2D), indicating that HO-1 may participate in RILI by regulating the function of macrophages.

Embodiment 3

[0033] Example 3 Determination of HO-1 expression at different times in lung macrophages induced by high-dose (6Gy, dose rate 600cGy / min) irradiation

[0034] Isolate mouse bone marrow-derived macrophages (BMDMs), and collect them after X-ray irradiation for different times (0, 1, 3, 6, 12, 24h), and detect the expression level of HO-1 by immunoblotting.

[0035] Experimental results: Western blotting found that HO-1 can be induced to express in X-ray irradiated macrophages, and the expression peak was reached at 6 hours ( image 3 )

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Abstract

The invention belongs to the field of molecular biological diagnosis and treatment of radiation-induced lung injury, and particularly relates to application of heme oxygenase-1 in diagnosis and treatment of radiation-induced lung injury. It is found that serious radiation-induced lung injury is caused by insufficient HO-1 content, so that for a patient subjected to large-dose X-ray radiation therapy, if HO-1 in blood is reduced, radiation-induced lung injury is caused. A drug such as Hemin for enhancing the expression and function of HO-1 can be used as a new method for treating radiation-induced lung injury.

Description

Technical field [0001] The invention belongs to the field of molecular biology diagnosis and treatment of radiation-induced lung injury, and specifically relates to the application of heme oxygenase-1 in the diagnosis and treatment of radiation-induced lung injury. Background technique [0002] Radiation-induced lung injury (RILI) is a very common complication after radiotherapy for thoracic malignancies, and it is a fatal disease characterized by interstitial pneumonia and pulmonary fibrosis. The clinical manifestations of RILI mainly include cough, shortness of breath, fever, respiratory insufficiency, and the development of severe respiratory failure. At present, only hormones combined with antibiotics, vitamins, antioxidants and other drugs can be used for treatment. Although they have achieved certain effects, they have caused many adverse reactions (see literature: Kalman NS, Zhao SS, Anscher MS, Urdaneta AI. Current Status of Targeted Radioprotection and Radiation Injury ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573A61K38/44A61K45/00A61P11/00A01K67/027
CPCG01N33/573A61K38/44A61K45/00A61P11/00C12Y114/99003A01K67/027A01K67/0276G01N2333/90274G01N2800/12A01K2207/35A01K2227/105A01K2267/03A01K2217/075
Inventor 庞柯欣李晓宇
Owner NANJING MEDICAL UNIV
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