Polypeptide single-layer film with primary amino exposure amount of 6%, and preparation method and application for polypeptide monolayer film

A single-layer membrane, primary amino technology, applied in the field of natural polymers, can solve the problem of limiting the application of polypeptide molecules, and achieve the effect of preventing postoperative infection, improving adhesion and proliferation, and maintaining good stability.

Active Publication Date: 2020-09-18
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the complexity of the structure of natural biological macromolecules, there are few reports on th

Method used

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  • Polypeptide single-layer film with primary amino exposure amount of 6%, and preparation method and application for polypeptide monolayer film
  • Polypeptide single-layer film with primary amino exposure amount of 6%, and preparation method and application for polypeptide monolayer film
  • Polypeptide single-layer film with primary amino exposure amount of 6%, and preparation method and application for polypeptide monolayer film

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Embodiment 1

[0054] A method for preparing a polypeptide monolayer film, comprising the following steps:

[0055] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0056] (2) Add surfactant SUS to the above-mentioned collagen polypeptide solution to obtain a collagen polypeptide-SUS mixed solution, the concentration of SUS in the mixed solution is 6.52mmol / L (at 50°C, the mass fraction of SUS in the mixed solution is 6%) ; Stable in a water bath for 6h for later use.

[0057] (3) Cut a rectangular titanium sheet with a size of 1cm×1c...

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Abstract

The invention provides a polypeptide single-layer film with a primary amino exposure amount of 6%, and a preparation method and an application for the polypeptide single-layer film. The polypeptide iscomposed of polypeptide molecules with a molecular weight of (1.48 +/- 0.2) * 10<5> g/mol; the thickness of the single-layer film is 14.6 +/- 0.1 nm; the exposure amount of primary amino groups on the surface of the film is 6 +/- 0.3%; the Zeta potential of the polypeptide single-layer film is minus 4.48 +/- 0.1 mV; and the contact angle of the film is 45 +/- 1 degrees. The polypeptide single-layer film provided by the invention has good stability; the exposure amount of primary amino groups on the surface of the film is accurately controlled to be 6.0 +/- 0.3%; and the polypeptide single-layer film can be applied to an oral implant surface coating material, and can carry antibiotics and antibacterial drugs and stably release the antibiotics and the antibacterial drugs; and the local drug-loaded coating of the polypeptide single-layer film has the advantages of small toxic and side effects on the whole body, strong antibacterial property and the like, is uniformly and tightly combinedwith a pure titanium material, and is not prone to separating and falling off.

Description

technical field [0001] The invention belongs to the field of natural polymers, and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with a primary amino group exposure of 6%, and its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Bio-immobilized coatings are often used in the field of biomimetic scaffolds to solve the problem of carrying biomolecules, drug molecules, synthetic polymers or small organic molecules such as enzymes, lactose, and polydopamine. The loading capacity is ea...

Claims

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Application Information

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IPC IPC(8): A61L27/06A61L27/34A61L27/50A61L27/54
CPCA61L27/06A61L27/34A61L27/54A61L27/50A61L2430/12A61L2300/406A61L2300/404C08L89/00C08L79/02
Inventor 李天铎许静马慧君班青张震
Owner QILU UNIV OF TECH
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