Polypeptide monolayer film with 7% of exposure quantity of primary amino groups as well as preparation method and application of polypeptide monolayer film

A single-layer film and primary amino technology, applied in the field of natural polymers, can solve problems such as limiting the application of polypeptide molecules, and achieve the effects of preventing postoperative infection, improving attachment and proliferation, and good stability

Active Publication Date: 2020-09-18
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the complexity of the structure of natural biological macromolecules, there are few reports on the surface properties of polypeptide monolayer membranes, which limits the application of polypeptide molecules.

Method used

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  • Polypeptide monolayer film with 7% of exposure quantity of primary amino groups as well as preparation method and application of polypeptide monolayer film
  • Polypeptide monolayer film with 7% of exposure quantity of primary amino groups as well as preparation method and application of polypeptide monolayer film
  • Polypeptide monolayer film with 7% of exposure quantity of primary amino groups as well as preparation method and application of polypeptide monolayer film

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Embodiment 1

[0054] A preparation method of a polypeptide monolayer film, comprising the following steps:

[0055] (1) Prepare 50 mL of collagen polypeptide solution with a concentration of 4% wt: accurately weigh collagen polypeptide into 100 mL in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-necked flask in a water bath at 50±1℃, heat and stir for 2h to make it completely dissolved, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize in the water bath for 0.5h.

[0056] (2) Add surfactant SDSO to the above collagen polypeptide solution to obtain a collagen polypeptide-SDSO mixed solution. The concentration of SDSO in the mixed solution is 8.04 mmol / L (at 50°C, the mass fraction of SDSO in the mixed solution is 6%) ; Stabilized in a water bath for 6h for later use.

[0057] (3) Cut a rectangular titanium sheet with a size of 1c...

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Abstract

The present invention provides a polypeptide monolayer film with 7% of exposure quantity of primary amino groups as well as a preparation method and application of the polypeptide monolayer film. Thepolypeptide is composed of a polypeptide molecule with the molecular weight of (1.48+ / -0.2)*105 g / mol, wherein the thickness of a monolayer film is 8.9+ / -0.1 nm, the primary amino group exposure of the surface of the film is 7.0+ / -0.2%, the Zeta potential of the polypeptide monolayer film is 0.76+ / -0.1 mV; and the contact angle of the film is 48+ / -1 DEG. The polypeptide monolayer film provided bythe present invention has good stability, may be applied as a coating material on the surface of an oral implant and may carry antibiotics and antibacterial drugs and stably release the antibiotics and the antibacterial drugs, the exposure quantity of the primary amino groups on the surface of the film is precisely controlled at 6.0+ / -0.3%, a local medicine carrying coating of the polypeptide monolayer film has the advantages such as little toxic and side effects to human bodies and strong antibacterial property, and the polypeptide monolayer film is uniform and tight in combination with a pure titanium material and not easy to separate and fall off.

Description

technical field [0001] The invention belongs to the field of natural macromolecules, relates to a polypeptide monolayer film, a preparation method and application thereof, and in particular relates to a polypeptide monolayer film with a primary amino group exposure of 7%, and a preparation method and application thereof. Background technique [0002] Collagen polypeptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen polypeptide is widely used in the preparation of medical materials, biomimetic materials, packaging and finishing materials. Drug molecules; the loading of synthetic polymers or small organic molecules. If collagen polypeptides are prepared into polypeptide monolayers, the loading amount can be easily and accurately controlled. [0003] However...

Claims

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Application Information

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IPC IPC(8): A61L27/06A61L27/34A61L27/50A61L27/54
CPCA61L27/06A61L27/34A61L27/54A61L27/50A61L2430/12A61L2300/406A61L2300/404C08L89/00C08L79/02
Inventor 许静李天铎班青马慧君张震
Owner QILU UNIV OF TECH
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