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Application of ganoderma lucidum peptide polysaccharide in preparation of skin histamine inhibitor

A Ganoderma lucidum peptidoglycan and secretion inhibitor technology, which is applied in the field of medicine, can solve the problems of Ganoderma lucidum peptidoglycan histamine inhibitors and hyaluronidase inhibitors that have not been reported, and achieve the purpose of inhibiting skin allergic reactions, inhibiting cell degranulation, The effect of good therapeutic effect

Inactive Publication Date: 2020-09-18
广东华夏友美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic and foreign scholars' research on Ganoderma lucidum peptidoglycan mainly focuses on anti-aging, anti-free radical, anti-tumor, immune regulation, etc. to report

Method used

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  • Application of ganoderma lucidum peptide polysaccharide in preparation of skin histamine inhibitor
  • Application of ganoderma lucidum peptide polysaccharide in preparation of skin histamine inhibitor
  • Application of ganoderma lucidum peptide polysaccharide in preparation of skin histamine inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation of Ganoderma lucidum peptidoglycan extract, its activity screening and content determination

[0051] 1. Materials and Instruments

[0052] Materials: Glucose (Fluck), Chizhi (purchased from Guangzhou Tongrentang), deionized water (self-made), concentrated sulfuric acid (Sinopharm Chemical Reagent Co., Ltd.), phenol (Sinopharm Chemical Reagent Co., Ltd.), and other reagents were of analytical grade.

[0053] Instruments: organic membrane filtration equipment (Shandong Bona Biotechnology Group Co., Ltd.), UV 2301-II ultraviolet spectrophotometer (Shanghai Tianmei Scientific Instrument Co., Ltd.); TGL-16 high-speed low-temperature centrifuge (developed by Hunan Xiangyi Laboratory Instruments) Co., Ltd.), Waters-Tag amino acid automatic analyzer (Waters Co., Ltd., USA).

[0054] Reagent: 5% phenol solution, take an appropriate amount of phenol, distill in a sand bath, collect fractions at 180-182°C, weigh 12.5g of the above fractions, dissolve in wa...

Embodiment 2

[0083] Example 2: Experiment on the inhibitory effect of Ganoderma lucidum peptidoglycan on histamine phosphate

[0084] 1. Materials and Instruments

[0085] Reagent: The preparation method of Ganoderma lucidum peptidoglycan is the same as that in Example 1.

[0086] Animals: Normal guinea pigs, 9 weeks old, weighing 260-280g, 48 males, provided by Longgui Xingke Animal Farm, Baiyun District, Guangzhou [certificate number: SCXK (Guangdong) 2018-0002].

[0087] 2. Experimental steps

[0088] Inhibitory activity of different doses of Ganoderma lucidum peptidoglycan on histamine phosphate

[0089] Take 48 normal-grade guinea pigs, half male and half female, weighing about 300g, and randomly divide them into 6 groups according to body weight, 8 in each group, namely the model group (Mol), the blank matrix group (CON), and the positive group is Fucinolone (FQS ), the concentration is 0.5%; Ganoderma lucidum peptidoglycan high dose group (10%), Ganoderma lucidum peptidoglycan midd...

Embodiment 3

[0093] Example 3: Ganoderma lucidum peptidoglycan inhibition experiment on hyaluronidase

[0094] Further, the antihistamine effect of Ganoderma lucidum peptidoglycan was verified through the inhibition of hyaluronidase.

[0095] 1. Materials and Instruments

[0096] Reagents: Ganoderma lucidum peptidoglycan (the above extract), hyaluronidase, sodium hyaluronate, and dipotassium glycyrrhizinate were all purchased from McLean Reagent Company. Absolute ethanol, sodium hydroxide, anhydrous sodium carbonate, concentrated hydrochloric acid, p-dimethylaminobenzaldehyde, acetylacetone, glacial acetic acid, sodium acetate crystallization, anhydrous calcium chloride, etc. are domestic analytically pure.

[0097] Preparation of test solution: ①. Acetic acid buffer solution (pH=5.6): measure 1.155mL of glacial acetic acid and dilute to 100mL, mix well, then take 4.8mL of it as solution A; weigh 2.72g of sodium acetate crystals, add water to dissolve and dilute to 100mL and mix well The...

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Abstract

The invention discloses application of ganoderma lucidum peptide polysaccharide in preparation of a skin histamine inhibitor. The molecular weight of the ganoderma lucidum peptide polysaccharide is 800Da-15KDa; simultaneously, the ganoderma lucidum peptide polysaccharide can be used for preparing a skin histamine secretion inhibitor and a hyaluronidase inhibitor, and has good treatment effect on related diseases thereof, in particular allergic dermatitis; and the ganoderma lucidum peptide polysaccharide and main components thereof have a certain inhibiting effect on histamine secretion, and simultaneously, can inhibit hyaluronidase to further inhabit histamine secretion. The ganoderma lucidum peptide polysaccharide in the invention is safe and free from stimulation and side effect, has a certain preventive and therapeutic effect on related diseases of skin histamine secretion, and can be widely applied to preparing a histamine inhibitor for skin application and a product for preventingand treating related diseases of skin histamine secretion.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of ganoderma lucidum peptidoglycan in the preparation of skin histamine inhibitors. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is the fruiting body of Ganoderma lucidum, a polyporaceae fungus. It is widely distributed in Zhejiang, Guangdong, Jiangxi and other places in China. It mainly grows on decayed wood or the roots of trees. Yellow Ganoderma, Purple Ganoderma, White Ganoderma and Black Ganoderma etc. Among them, Ganoderma lucidum (Leyss.ex Fr.) Karst, also known as Danzhi and Chizhi, is corky, with a kidney-shaped or semicircular cap, reddish-brown and shiny surface, and the color of the stipe is average. Darker, it has nourishing, strong and anti-inflammatory effects in traditional Chinese medicine. Chizhi contains triterpenes such as ganoderma acid A, B, C 1 、C 2 、D 1 , Chizhi acid A, B, C, D 1 , Lingchi acid, etc.; polysacch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K36/074A61P17/00A61P37/08A61K8/73A61K8/9728A61Q19/00
CPCA61K8/73A61K9/0014A61K31/715A61K36/074A61K2800/782A61Q19/00A61K8/9728A61P17/00A61P37/08
Inventor 沈志滨胡锡昌张衍湖邓榕榕廖志强朱研芳
Owner 广东华夏友美生物科技有限公司
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