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Hydrophilic antigen and/or hydrophobic antigen vaccine delivery system and preparation method thereof

A delivery system and a hydrophobic technology, applied in the field of vaccine delivery system and its preparation, can solve the problems of poor lymph node targeted delivery effect and the like, and achieve the effect of a simple preparation method

Active Publication Date: 2020-09-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims at the problems that the existing vaccine vectors usually can only carry and deliver single-component antigens, which lead to the inability to induce a comprehensive immune response against pathogenic antigens, often need to be combined with other adjuvants, and poor lymph node targeted delivery. A vaccine delivery system that can carry and deliver multiple antigen components individually or simultaneously. By delivering the antigen to the lymph node, it can promote the uptake and presentation of the antigen by the antigen-presenting cells in the lymph node, thereby inducing a vaccine that is stronger than a single-component antigen. Immune response to vaccine delivery system or free antigen

Method used

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  • Hydrophilic antigen and/or hydrophobic antigen vaccine delivery system and preparation method thereof
  • Hydrophilic antigen and/or hydrophobic antigen vaccine delivery system and preparation method thereof
  • Hydrophilic antigen and/or hydrophobic antigen vaccine delivery system and preparation method thereof

Examples

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Effect test

Embodiment 1

[0052] Dissolve 6 g of cetyltrimethylammonium chloride and 0.18 g of triethanolamine in sterile water, heat and stir for 1 h, add 20 ml of tetraethyl orthosilicate / cyclohexane solution (v / v, 5%), Adjust the stirring speed to 180rpm, react for 24 h, the product is generated in the water phase, the water phase is diluted with ethanol, centrifuged, and the precipitate is collected, the precipitate is washed twice with absolute ethanol, and resuspended with 40 ml of ammonium nitrate / ethanol solution (w / v, 0.6%), heated and stirred to reflux for 6 h to remove the template agent cetyltrimethylammonium chloride; the refluxed mesoporous silica was collected by centrifugation and freeze-dried.

Embodiment 2

[0054] Dissolve 6 g of cetyltrimethylammonium chloride and 0.8 g of triethanolamine in sterile water, heat and stir for 1 h, add 20 ml of tetraethylorthosilicate / naphthalene solution (v / v, 20%), and dissolve Adjust the stirring speed to 300rpm, react for 12 hours, the product is formed in the water phase, the water phase is diluted with ethanol, centrifuged, and the precipitate is collected, the precipitate is washed twice with absolute ethanol, and resuspended with 40 ml of ammonium nitrate / ethanol solution (w / v, 0.6%), heated and stirred to reflux for 6 h to remove the template agent cetyltrimethylammonium chloride; the refluxed mesoporous silica was collected by centrifugation and freeze-dried.

Embodiment 3

[0056] Dissolve 6 g of cetyltrimethylammonium chloride and 0.05 g of triethanolamine in sterile water, heat and stir for 1 h, add 20 ml of tetraethylorthosilicate / naphthalene solution (v / v, 40%), and dissolve The stirring speed was adjusted to 100 rpm, and the reaction was carried out for 1 h. The product was formed in the water phase. The water phase was diluted with ethanol, centrifuged, and the precipitate was collected. The precipitate was washed twice with absolute ethanol, and resuspended with 40 ml of ammonium nitrate / ethanol solution (w / v, 0.6%), heated and stirred to reflux for 6 h to remove the template agent cetyltrimethylammonium chloride; the refluxed mesoporous silica was collected by centrifugation and freeze-dried.

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Abstract

The invention provides a hydrophilic antigen and / or hydrophobic antigen vaccine delivery system and a preparation method thereof. According to the invention, mesoporous silica with good dispersibilityand controllable particle size and pore diameter is synthesized by adopting a two-phase method. A hydrophilic antigen is entrapped by the mesoporous silica through mesopores, a hydrophobic antigen isentrapped by a lipid membrane, and the vaccine delivery system is a multivalent antigen delivery system. The prepared vaccine can be transferred to lymph nodes, is efficiently taken by antigen presenting cells, induces antigen-specific immune response, and has a wide application prospect.

Description

technical field [0001] The invention relates to a mesoporous silica-based vaccine delivery system for hydrophilic antigens and / or hydrophobic antigens and a preparation method thereof, belonging to the field of medicine. Background technique [0002] The invention and application of vaccines is one of the greatest achievements of modern medicine, and it is of indisputable importance in the control and prevention of global infectious diseases. The three important directions of vaccine development include: the discovery of new antigens, new adjuvant technologies and new carrier platforms. [0003] As the most important component of vaccines, antigens are the key to determine the protective effect of vaccines. For a pathogen, a vaccine should include as many relevant antigens of the pathogen as possible to induce the body to produce a comprehensive and strong protective effect. However, the properties of different antigens derived from different pathogens or the same pathogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/104A61K39/39A61K47/69A61K47/04A61P35/00B82Y5/00A61P31/04
CPCA61K39/0011A61K39/104A61K47/6923A61K39/39B82Y5/00A61P35/00A61P31/04A61K2039/70A61K2039/55505A61K2039/57A61K2039/575
Inventor 孙逊张志荣洪小玉郭兆飞龚涛
Owner SICHUAN UNIV
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