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Novel coronavirus SARS-CoV-2 detection method and novel coronavirus SARS-CoV-2 detection device

A coronavirus and detection method technology, applied in the field of biomedicine, can solve the problems of long detection time, false positive nucleic acid detection, and high requirements for detection equipment, and achieve the effect of high accuracy, fast detection speed, and sample detection.

Active Publication Date: 2020-09-11
苏州微湃医疗科技有限公司
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  • Application Information

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Problems solved by technology

Existing detection technologies are mainly divided into nucleic acid detection and antibody detection. First, nucleic acid detection has the problems of false positives, long detection time and limited detection environment. At the same time, it has high requirements for detection equipment, platforms and operators. The second antibody Most of the tests use colloidal immunogold chromatography technology to detect patient samples on test strips to qualitatively determine whether the samples contain novel coronavirus antibodies, which cannot be quantitatively and accurately determined

Method used

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  • Novel coronavirus SARS-CoV-2 detection method and novel coronavirus SARS-CoV-2 detection device

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Embodiment 1

[0033] refer to Figure 5 As shown, this embodiment detects the novel coronavirus SARS-CoV-2 (full name is: Severe Acute Respiratory Syndrome Coronavirus 2 in 96 samples to be tested) with a desktop Raman spectrometer in a laboratory environment, and the Chinese interpretation is Severe Acute Respiratory Syndrome 2) Monospecific IgG antibody as an example:

[0034] S1. Surface-enhanced Raman scattering substrate 1 was prepared from nanofibrillated cellulose paper by oxidation treatment of 2,2,6,6-tetramethylpiperidine oxide, refer to Figure 4 Shown, concrete preparation method is as follows:

[0035] S1-1. adding the original slurry of nanofibrillated cellulose oxidized by 2,2,6,6-tetramethylpiperidinium oxide into distilled water to prepare a suspension with a cellulose content of 0.1%;

[0036] S1-2. Place the prepared suspension in a mixer and stir at a speed of 1000rpm for 2 hours;

[0037] S1-3. Take out 50g of the suspension and vacuum filter it on the filter holder ...

Embodiment 2

[0051] In this embodiment, the specific IgG antibody and IgM antibody of the new coronavirus SARS-CoV-2 in 48 samples to be tested are detected with a desktop Raman spectrometer in a laboratory environment as an example:

[0052] S1. Surface-enhanced Raman scattering substrate 1 is prepared from nanofibrillated cellulose paper by oxidizing 2,2,6,6-tetramethylpiperidine oxide. The specific preparation method and pattern of substrate 1 are the same as in Example 1 , the present invention will not go into details here;

[0053] S2. Activate the prepared substrate 1. The specific activation process is the same as in Example 1. After the activation is completed, add the new coronavirus antigen protein specific to human IgG or IgM antibodies to the 96 wells, and the addition is completed. Post-incubation for 30 minutes;

[0054] S3. Divide each of the processed 48 samples to be tested into two parts to obtain 96 drop samples, which are sequentially added to 96 wells. If the samples...

Embodiment 3

[0059] In this embodiment, the specific IgG antibody of the new coronavirus SARS-CoV-2 in the sample to be tested is detected by a handheld portable Raman spectrometer in a non-laboratory complex environment as an example:

[0060] S1. Surface-enhanced Raman scattering substrate 1 is prepared by oxidation of nanofibrillated cellulose paper through 2,2,6,6-tetramethylpiperidine oxide. The specific preparation method is the same as that of Example 1, and the pattern of substrate 1 is limited to one hole position;

[0061] S2. Activate the prepared substrate 1. The specific activation process is the same as in Example 1. After the activation is completed, add the new coronavirus antigen protein specific to the IgG antibody to the hole, and incubate for 30 minutes after the addition is completed;

[0062] S3. Steps S1 and S2 need to be prepared in advance in the laboratory environment, and the prepared substrate 1 should be frozen and stored. During the actual detection, the subst...

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Abstract

The invention discloses a novel coronavirus SARS-CoV-2 detection method and a novel coronavirus SARS-CoV-2 detection device. A substrate is mainly prepared by oxidizing nano fibrillated cellulose paper by 2, 2, 6, 6-tetramethylpiperidine oxide. A substrate pattern is wax-printed on the surface of the substrate; qualitative detection of a novel coronavirus specific IgG antibody or IgM antibody in asample is realized on the substrate by utilizing an immunogold labeling technology; detection speed is fast, after calibration, the antibody concentration in the sample can be quantitatively detected; accuracy is high, the area of a surface-enhanced Raman scattering substrate for adding a reagent is not limited to the fixed substrate pattern; the size and style of the substrate pattern can be changed according to the use requirements and the detection environment, the detection of two novel coronavirus specific antibodies can be completed at the same time, and a plurality of samples to be detected can be detected at the same time, so that the sample detection is realized most efficiently.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a detection method and a detection device for the novel coronavirus SARS-CoV-2. Background technique [0002] The new coronavirus is a relatively highly contagious virus, which mainly causes the formation of viral pneumonia. Common signs include fever, fatigue, dry cough, and gradually difficulty in breathing. The virus has a strong incubation period in the human body, and the incubation period is also contagious. There are even a large number of asymptomatic people without clinical symptoms, and asymptomatic people may also be contagious. Therefore, how to quickly and accurately detect new coronaviruses in suspected and asymptomatic groups is an important issue in dealing with the virus. Existing detection technologies are mainly divided into nucleic acid detection and antibody detection. First, nucleic acid detection has the problems of false positives, long detection time...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 栗昕马海波岳春峰刘新宇
Owner 苏州微湃医疗科技有限公司
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