A Saline-alkali-tolerant New Trichoderma and Its Application
A salt-alkali and Trichoderma-resistant technology, applied in the biological field, can solve the problem of less Trichoderma and its preparations
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Embodiment 1
[0029] Example 1: Isolation and identification of tidal flat Trichoderma TUCIM 10301
[0030] Tidal flat Trichoderma TUCIM 10301 was isolated from the rhizosphere soil of Phragmites reed in coastal flats of Dafeng Port, Jiangsu Province, and the preservation number is CGMCC NO.19611.
[0031] 1.1 Morphological characteristics of tidal flat Trichoderma TUCIM 10301
[0032] 1.1.1 Test medium
[0033] PDA: potato dextrose agar medium;
[0034] CMA: corn flour agar medium;
[0035] SNA: Synthetic Low Nutrient Agar Medium (Recipe: KH 2 PO 4 1.0g l -1 ; KNO 3 1.0g l -1 ;MgSO 4 ·7H 2 O0.5 g l -1 ;KCl 0.5g l -1 ; Glucose 0.2g l -1 ;Sucrose 0.2g l -1 ;Agar powder 20g l -1 ; pH adjusted to 7.0)
[0036] 1.1.2 Colony morphology
[0037] Inoculate the TUCIM 10301 strain of tidal flat Trichoderma TUCIM 10301 on the above-mentioned medium, and then culture it under dark conditions at 25°C for 3 days to observe its morphology. figure 2 ):
[0038] Colony: Trichoderma TUC...
Embodiment 2
[0044] Embodiment 2: the saline-alkali tolerance of tidal flat Trichoderma TUCIM 10301
[0045] Phragmites reed can grow normally in coastal saline soil with a salt content of 1.5% and pH = 8.5, so Trichoderma isolated from the rhizosphere soil samples of Phragmites is highly likely to tolerate high-salt and high-alkaline environments. From the Trichoderma screened, select the strains with fast growth and many spores to carry out NaCl and pH tolerance test as follows:
[0046] 2.1 Salt tolerance test:
[0047] Taking the tidal flat Trichoderma TUCIM 10301 strain as the main research object, another two fast-growing, spore-forming Trichoderma strains were selected to conduct salt tolerance tests together. Inoculate the three bacteria on the PDA plate at the same time, culture at 28°C for 7 days, after producing a large number of conidia, scrape the spores under sterile conditions and dilute them to OD with sterile water 600 = 0.1. First distribute 30% Murashige Skoog medium ...
Embodiment 3
[0052] Example 3: Inhibitory effect of tidal flat Trichoderma TUCIM 10301 on various plant pathogenic fungi
[0053] Four plant pathogenic fungi were selected, including Pestalotiopsis fici TUCIM 5788, Rhizoctonia solani TUCIM 3753, Fusarium oxysporum TUCIM4848, Alternaria alternata TUCIM 10233), the above-mentioned phytopathogenic bacteria were inoculated on one side of a 9 cm PDA plate with a sterilized hole puncher with a diameter of about 5 mm, and inoculated Trichoderma TUCIM 10301 on the other side of the plate after 24 hours, and cultivated in the dark at 25 ° C. On 14d, take pictures to record the situation of fungal confrontation.
[0054] The results show( Figure 4 ), Trichoderma TUCIM 10301 can inhibit the growth of Alternaria and Rhizoctonia solani colonies, limit the colony radius to 1.5-2.0cm, and completely cover the colonies of these two pathogenic fungi, producing A small amount of Trichoderma spores; Trichoderma TUCIM 10301 antagonizes Fusarium oxysporum a...
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