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A saline-alkali tolerant Trichoderma aculeatus and its application

A technology of Trichoderma aculeatus and salt-alkali resistance, which is applied in the biological field and can solve the problems of few microbial groups, difficulty in rooting and standing of crops, poor physical and chemical properties, etc.

Active Publication Date: 2021-11-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of organic matter in saline soil, poor physical and chemical properties, few microbial groups, many anions and cations harmful to crops, and high osmotic pressure of soil solution, it is difficult for crops to take root and stand up. Therefore, how to promote the growth of plant roots in saline soil is the key technology.

Method used

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  • A saline-alkali tolerant Trichoderma aculeatus and its application
  • A saline-alkali tolerant Trichoderma aculeatus and its application
  • A saline-alkali tolerant Trichoderma aculeatus and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of Trichoderma aculeatus TUCIM 10320

[0029] Trichoderma aculeatus TUCIM 10320 was isolated from the rhizosphere soil of reeds on the coastal flats of Dafeng Port, Jiangsu Province. The strain could cover a plate with a diameter of 9 cm when cultured on PDA medium at 28°C for 72 hours, and the aerial hyphae were white and long hairy. , producing a large number of dark green conidia on the front of the colony. After morphological identification and molecular identification based on ITS, rpb2, and tef1 gene fragments, the strain was Trichoderma asperelloides (Trichoderma asperelloides), which was finally named Trichoderma asperelloides TUCIM 10320, see the evolutionary tree figure 1 .

[0030] The Trichoderma aculeatus is screened in coastal saline soil, which is friendly to the local environment; it produces a large amount of spores, which is convenient for agricultural production and utilization, and the indigenous Trichoderma h...

Embodiment 2

[0032] Embodiment 2: the saline-alkali tolerance of Trichoderma aculeatus TUCIM 10320

[0033] Phragmites reed can grow normally in coastal saline soil with a salt content of 1.5% and pH = 8.5, so Trichoderma isolated from the rhizosphere soil samples of Phragmites is highly likely to tolerate high-salt and high-alkaline environments. From the Trichoderma screened, select the strains with fast growth and many spores to carry out NaCl and pH tolerance test as follows:

[0034] 2.1 Salt tolerance test:

[0035] Trichoderma aculeatus TUCIM 10320 strain was used as the main research object, and two other fast-growing, sporogenous Trichoderma strains were selected for salt tolerance test. Inoculate the three bacteria on the PDA plate at the same time, culture at 28°C for 7 days, after producing a large number of conidia, scrape the spores under sterile conditions and dilute them to OD with sterile water 600 = 0.1. First, distribute PDB medium (potato dextrose medium, without aga...

Embodiment 3

[0040] Example 3: Inhibitory effect of Trichoderma aculeatus TUCIM 10320 on various plant pathogenic fungi

[0041] Five plant pathogenic fungi were selected, including Pestalotiopsis fici TUCIM 5788, Rhizoctonia solani TUCIM 3753, Fusarium oxysporum TUCIM4848, Alternaria alternata TUCIM 10233), large stem point mold (Macrophomasp.TUCIM 10254), the above-mentioned phytopathogenic bacteria are inoculated on one side of the PDA plate of 9cm with a sterilized hole puncher with a diameter of about 5mm, and Trichoderma aculeatus is inoculated after 24h TUCIM 10320 was cultured on the other side of the plate in the dark at 25°C for 14 days, and the fungal confrontation was recorded by taking pictures.

[0042] The results show( image 3 ), Trichoderma aculeatus TUCIM 10320 can inhibit the growth of Phomama, Alternaria, and Rhizoctonia solani colonies, limiting the colony radius to 1-1.5cm, and completely covering these three pathogenic fungi A small amount of Trichoderma spores we...

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Abstract

The invention discloses a saline-alkali-resistant trichoderma aculeatus and application thereof. Trichoderma asperelloides TUCIM 10320 was deposited on April 3, 2020 in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the deposit number CGMCC 19612. Trichoderma aculeatus TUCIM 10320 can tolerate salt stress caused by a salt concentration of 1.5M (ie 8%) and alkali stress with a pH value of 9.0, and has good salt-alkali tolerance. Under different salt concentration stress, Trichoderma aculeatus TUCIM 10320 relieved the effect of salt on plant growth inhibition. Compared with the control without inoculation, inoculation of TUCIM 10320 increased the crop biomass by 30% under the conditions of 0.5% and 0.75% NaCl content respectively. % and 62%, to promote crop root elongation, lateral root differentiation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a saline-alkali-tolerant Trichoderma aculeatus and its application. Background technique [0002] There are about 1.5 billion mu of saline soil in my country, which is the main reserve land resource in our country. With the acceleration of its natural desalination and artificial improvement, the agricultural utilization of saline soil has become an important part of my country's agricultural technology and production. However, due to the low content of organic matter in saline soil, poor physical and chemical properties, few microbial groups, many anions and cations harmful to crops, and high osmotic pressure of soil solution, it is difficult for crops to take root and stand up. Therefore, how to promote the growth of plant roots in saline soil is the key technology. . [0003] Trichoderma spp. belongs to Ascomycota (Ascomycota) Hypocreales, is a kind of ubiquitous and easy to isolate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12N1/145C12R2001/885
Inventor 丁明月蔡枫陈巍伊瑞娜·杜鲁兹尼娜
Owner NANJING AGRICULTURAL UNIVERSITY
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