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The osabcg48 gene and its application in improving the resistance of unicellular organisms and plants to cadmium stress

A single-cell, biological technology, applied in the field of genetic engineering, can solve the problem of the cost of engineering is not operability, etc., and achieve significant specific effects

Active Publication Date: 2022-03-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the existing methods, soil remediation is mainly used as a treatment method to deal with cadmium pollution in soil, but neither soil cleaning nor soil replacement can do anything for large-scale pollution, and its engineering volume and huge cost make it basically impossible Operability

Method used

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  • The osabcg48 gene and its application in improving the resistance of unicellular organisms and plants to cadmium stress
  • The osabcg48 gene and its application in improving the resistance of unicellular organisms and plants to cadmium stress
  • The osabcg48 gene and its application in improving the resistance of unicellular organisms and plants to cadmium stress

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Embodiment Construction

[0040] In order to make the invention, the technical solution, and its technical effects are more clear, the present invention will be described in further detail below. It should be understood that the specific embodiments described in this specification are merely intended to illustrate the invention.

[0041] The experimental materials and reagents used are all consumables and reagents that are conventionally available from the commercial pathway without specifically described.

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Abstract

The invention discloses the OsABCG48 gene and its application in improving the resistance of unicellular organisms and plants to cadmium stress. The rice OsABCG48 protein sequence is as SEQ ID NO.1 or its substitution, deletion and / or addition of one or more amino acids and The gene encoding rice OsABCG48 protein is shown in SEQ ID NO. The invention finds that OsABCG48 gene can be overexpressed in single-cell organisms and plants by introducing OsABCG48 gene into single-cell organisms and plants, and improves the resistance to cadmium stress. The OsABCG48 gene of the present invention has extremely high development potential in the cultivation of new strains of cadmium-resistant single-cell organisms and new strains of crops resistant to cadmium.

Description

Technical field [0001] The present invention belongs to the field of genetic engineering, and the OSABCG48 gene is involved in improving single cell biology and higher plants, including crop anti-cadmium capabilities. Background technique [0002] Cadmium (CD) is a toxic heavy metal and one of the most environmentally threatened pollutants and one of the elements that are maximized living cell toxicity. As a biological non-essential metal, it can replace other essential metals, after entering the human body, causing internal ion steady state imbalances, protein dysfunction, DNA and plasma membrane injury, and accumulation of active oxygen. Cadmium is also a carcinogen, cadmium accumulation can cause bone fragility and renal failure. Cadmium mainly depends on food intake. Cadmium has a longer bio-half-life in the kidney (about 10-30 years), even if long-term low dose intake can lead to chronic poisoning. [0003] Existing studies have shown that the plant source diet CD is the mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/70C12N15/81C12N15/84C12N1/21C12N1/19A01H5/00A01H6/20C12R1/645C12R1/19
CPCC07K14/415C12N15/8271C12N15/70C12N15/815
Inventor 王昌虎蔡兴喆区永祥
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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