Amomum tsao-ko tissue culture and seedling raising method
A technology of grass fruit and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of cultured tissue death, yellowing death, less research, etc., to avoid callus production, reduce Callus stage, the effect of improving propagation efficiency
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Embodiment 1
[0043] 1. Selection and cleaning of explants
[0044] Screen the germplasm with large fruit and high content of aromatic oil as the female parent, cut the young and tender stem section of the base of the healthy, pest-free herbaceous fruit stem, cut it into 2-3 cm long, place it under tap water and rinse it for 1 hour, Rinse with soap solution for 10 minutes, then rinse with clean water 2-3 times until no soap solution remains, and finally rinse under tap water for 1-2 hours.
[0045] 2. Disinfection of explants
[0046] Put the cleaned explants in the ultra-clean bench, first disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 3 times, then disinfect with 8% hydrogen peroxide for 12 minutes, rinse with sterile water for 3 to 4 times, and finally rinse with 0.1 % mercury chloride + 2 drops of Tween-80 disinfection for 10 minutes, rinsed with sterile water 8 times, the contamination rate was 13.2% within 7 days, and the mortality rate of explants was 2.7%. ...
Embodiment 2
[0054] 1. Selection and cleaning of explants
[0055] The explant used in the present invention is the young and tender stem section of the grass fruit stem base, which is cut into 2 to 3 cm long, placed under tap water for 1 hour, rinsed with soap solution for 10 minutes, and then rinsed with water for 2 to 3 minutes. Rinse until no soap remains, and finally rinse under tap water for 1 to 2 hours.
[0056] 2. Disinfection of explants
[0057] Place the cleaned explants in an ultra-clean bench, first disinfect with 75% alcohol for 30 seconds, rinse with sterile water for 3 times, then disinfect with 8% hydrogen peroxide for 11 minutes, rinse with sterile water for 3 times, and finally rinse with 0.1% L Mercury + 2 drops of Tween-80 disinfection for 15 minutes, rinsed with sterile water 8 times, the contamination rate was 10.7% within 7 days, and the mortality rate of explants was 5.2%.
[0058] 3. Induction of explants
[0059] Blot the surface moisture of the sterilized ex...
Embodiment 3
[0065] 1. Selection and cleaning of explants
[0066] Screen the germplasm with large fruit and high content of aromatic oil as the female parent, cut the young and tender stem section of the base of the healthy, pest-free herbaceous fruit stem, cut it into 2-3 cm long, place it under tap water and rinse it for 1 hour, Rinse with soap solution for 10 minutes, then rinse with clean water 2-3 times until no soap solution remains, and finally rinse under tap water for 1-2 hours.
[0067] 2. Disinfection of explants
[0068] Place the cleaned explants in the ultra-clean bench, first disinfect with 75% alcohol for 15 seconds, rinse with sterile water for 3 times, then disinfect with 8% hydrogen peroxide for 10 minutes, rinse with sterile water for 3 to 4 times, and finally rinse with 0.1 % mercury chloride + 2 drops of Tween-80 disinfection for 15 minutes, rinsed with sterile water 8 times, the contamination rate was 13.2% within 7 days, and the mortality rate of explants was 4.7%...
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