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Rapid sulfathiazole detection method

A technology for sulfathiazole and a detection method, which is applied in the field of rapid detection of sulfathiazole in animals, can solve the problems that it is difficult to meet the requirements of rapid screening of samples, the sensitivity and stability need to be improved, and there are many factors affecting the enzyme-linked immunosorbent assay, and the rapid detection of sulfathiazole is achieved. The effect of the convenient detection method

Pending Publication Date: 2020-08-25
詹云丹
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Problems solved by technology

Chromatography and mass spectrometry generally have defects such as long pretreatment process, complicated and technical operation, and expensive instruments and equipment, which make it difficult to meet the requirements for rapid screening of a large number of samples on site
Enzyme-linked immunoassay has a large sample size, low cost, and simple and portable instruments, but there are many factors affecting the enzyme-linked immunosorbent assay, and the sensitivity and stability need to be improved

Method used

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  • Rapid sulfathiazole detection method
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Embodiment Construction

[0028] In order to express the present invention more clearly, the present invention will be further described below in conjunction with the accompanying drawings.

[0029] Surface-enhanced Raman spectroscopy refers to the use of gold and silver nanoparticles as carriers. When the molecules to be tested are adsorbed on the rough gold and silver surfaces, the surface area between light and molecules will be greatly increased, and the electromagnetic field on the metal surface will also increase. The amplitude is enhanced, and the obtained Raman signal strength is ordinary 10 6 more than double. Surface-enhanced Raman spectroscopy has the advantages of simple pretreatment of experimental samples, fast detection of a single sample, and high detection sensitivity. Therefore, it has been widely used in the rapid monitoring of quality and safety issues such as trace pesticide residues and antibiotic drug residues in food and agricultural products. in the state of the art.

[0030]...

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Abstract

The invention provides a rapid detection method of sulfathiazole in an animal body, and a Raman characteristic peak of sulfathiazole is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. According to the method, the lowest detection concentration of sulfathiazole in an animal body is 1 mg / L. According to the method, the sulfonamide antibiotic residues in the animal body are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide thiazole residues in the animal body.

Description

technical field [0001] The invention relates to the field of rapid biological detection, in particular to a rapid detection method for sulfathiazole in animals. Background technique [0002] Veterinary drug residues refer to the parent compound and / or its metabolites of veterinary drugs contained in any edible part of animal products, as well as impurities related to veterinary drugs. These include antibiotics, anthelmintics, antiprotozoals, trypanosomidates, etc. [0003] Veterinary drugs and feed additives left in animal-derived food enter the human body along the food chain, posing a potential threat to human health. This threat has attracted more and more attention. With the change of people's demand for animal-derived food, the international requirements for drug residues in animal-derived food are getting higher and higher. Drug residues in animal-derived food have gradually become a focus of attention worldwide, among which antibiotic residues are subject to Great a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 詹云丹
Owner 詹云丹
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