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Preparation method of araboxylan and product

A technique of extracting arabinoxylan, which is applied in the field of arabinoxylan preparation, can solve the problems of weakening functional properties, destroying functional groups, and low extraction rate, so as to achieve increased added value, high purity, and improved dissolution rate Effect

Active Publication Date: 2020-08-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In comparison, the extraction rate of the water extraction method is generally low; although the extraction rate of the alkaline extraction method is relatively high, it usually destroys some functional groups in the AX structure, such as feruloyl groups, protein groups, etc., It may weaken its functional properties; the conditions of enzymatic extraction are relatively mild, which will partially degrade the sugar chain structure of AX, but generally will not affect the functional groups in it

Method used

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  • Preparation method of araboxylan and product
  • Preparation method of araboxylan and product
  • Preparation method of araboxylan and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of water-soluble arabinoxylans with different molecular weights:

[0044] (1) 100 meshes of the purchased whole wheat flour are sieved to obtain wheat bran;

[0045] (2) Take 500g of wheat bran, mix it with deionized water, soak it at 4°C for 60min, then wash the wet wheat bran with water with a mass concentration of 5% to remove starch, and drain the washed wheat bran , heated in an oven at 50°C for 12 hours to obtain destarched wheat bran and store it in an environment of 4°C;

[0046] (3)① Mix 150g of dried starch-free wheat bran with 2.25L of 0.15M NaOH solution, wherein the NaOH solution contains 0.5% H 2 o 2 , reacted at 80°C for 90 minutes, then cooled the mixture to room temperature and centrifuged for 30 minutes to obtain supernatant a; ② adjusted the pH of supernatant a to 4.5 with 4M HCl, and then centrifuged for 30 minutes to obtain supernatant b; The supernatant b was concentrated under reduced pressure to 1 / 4 of the original volume, then pre...

Embodiment 2

[0056] The molecular weights of the polysaccharides M60%-2, M70%-2, M80%-2, N40%-2 and N70%-2 prepared in Example 1 were determined by high performance gel permeation chromatography (GPC). The chromatographic conditions are: TSK gel G3000PWxL (300*7.8mm, 7um) chromatographic column is used; the mobile phase used is 0.05M mol / L sodium sulfate aqueous solution; the flow rate of the mobile phase is 1.0ml / min; the column temperature is 35°C; the detector is RID differential detector. The relative molecular mass of the dextran reference substance is 3000, 10000, 40000, 70000, 500000, and it is mixed with the polysaccharide sample to form a 1.0 mg / ml solution. After filtering, after the instrument chromatographic column is balanced and the baseline is stable, inject 20 μl of the sample, as Figure 1-2 and Table 1.

[0057] Molecular weight is an important parameter of polysaccharides, which is closely related to the physicochemical properties and biological activities of polysaccha...

Embodiment 3

[0062] The polysaccharides M60%-2, M70%-2, M80%-2, N40%-2, and N70%-2 stored in Example 1 were analyzed by ultraviolet-visible scanning spectrum.

[0063] Since proteins and nucleic acids have ultraviolet absorption functions at specific wavelengths, the full-wavelength ultraviolet scanning analysis of polysaccharides can be used to identify whether proteins and nucleic acids are contained in polysaccharide solutions. Prepare polysaccharides M60%-2, M70%-2, M80%-2, N40%-2, and N70%-2 respectively into 5mg / ml polysaccharide solutions, and scan them with a UV spectrophotometer in the range of 200-400nm , the scan results are as follows image 3 shown.

[0064] according to image 3 It can be seen that the polysaccharide solutions M60%-2, M70%-2, M80%-2, N40%-2, and N70%-2 have no characteristic absorption peaks of nucleic acid and protein near 260nm and 280nm, indicating that the five polysaccharide solutions The removal effect of protein and nucleic acid is better, and the a...

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Abstract

The invention discloses a preparation method of araboxylan. The preparation method specifically comprises the following steps: (1) removing starch from wheat bran; (2) obtaining a polysaccharide solution by adopting an alkali extraction method; (3) carrying out deproteinization treatment on the polysaccharide solution; (4) carrying out enzyme treatment and dialysis on the deproteinized polysaccharide solution; (5) carrying out enzymolysis on the dialyzed polysaccharide solution; (6) respectively carrying out alcohol precipitation classification on the polysaccharide solution which is not subjected to enzymolysis and the polysaccharide solution which is subjected to enzymolysis to obtain araboxylan with different molecular weights; and (7) carrying out secondary enzyme treatment and secondary dialysis on araboxylan with different molecular weights. The preparation method is simple and feasible, the utilization rate of the raw material wheat bran can be increased, araboxylan with different fractions can be obtained, the structures are different, the functional effects of reducing blood sugar, resisting oxidation and the like to different extents are achieved, and the medical value ishigh.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method and product of arabinoxylan. Background technique [0002] Arabinoxylan (arabinoxylans, AX) is a non-starch polysaccharide of great research value, mainly found in the seed coats of wheat, rye, barley, rice, corn and other grains, and is one of the main components of plant cell walls. AX has a complex structure and a wide molecular weight distribution. The basic structure includes a xylan backbone connected by β-D-xylopyranose residues through β-(1→4) glycosidic bonds and α-L-arabinofuranosose The difference in the relative number and substitution of arabinose residues is the main reason for the differences in the properties of arabinoxylan (solubility, solution viscosity, gelling property, degree of enzyme action, etc.). [0003] According to the different solubility of AX in water, it is usually divided into two categories: water-extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/14
CPCC08B37/0003C08B37/0057
Inventor 牛猛王朦唐翠娥张宾佳贾才华赵思明
Owner HUAZHONG AGRI UNIV
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