Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus coagulans BC99 and screening method thereof

A technology of Bacillus coagulans and screening methods, applied in the field of microorganisms, can solve the problems of large losses and poor stress resistance of probiotic strains, and achieve the effects of high survival rate, strong stress resistance, and high tolerance

Inactive Publication Date: 2020-08-18
武汉微康益生菌研究院有限公司
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the widely used probiotics are mainly Bifidobacterium, Lactobacillus acidophilus, Enterococcus faecalis and Enterococcus faecium, etc., but the above-mentioned probiotic strains have poor stress resistance, and the loss during processing is relatively large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus coagulans BC99 and screening method thereof
  • Bacillus coagulans BC99 and screening method thereof
  • Bacillus coagulans BC99 and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening and identification of Bacillus coagulans BC99

[0026] Take 1.0g of fresh baby feces sample, put it into a sterile centrifuge tube filled with 9.0mL of sterile water, oscillate and mix well, place the obtained bacterial suspension in a water bath in boiling water at 80°C for 30min, take it out; then dilute it with a 10-fold gradient 100 μL of diluent with appropriate concentration gradient was selected, and MRS agar with 0.25% calcium carbonate was added (peptone 10g, beef extract powder 10g, yeast powder 5g, glucose 20g, KH 2 PO 4 2g, diammonium hydrogen citrate 2g, anhydrous sodium acetate 5g, MgSO 4 0.2g, MnSO 4 0.6g, Tween80 1mL, agar 15g, distilled water 1000mL, pH6.0-6.2) on a plate, spread evenly with a sterile coating rod, use sterile water as a blank control, spread evenly and place in a 42°C constant temperature incubator Inverted for 48-72h. Pick white or off-white, glossy, round, neatly edged, and colonized strains, and perform repeated stre...

Embodiment 2

[0029] Colony Morphology and Microscopic Examination of Bacillus coagulans BC99

[0030] Carry out colony culture to Bacillus coagulans BC99, observe the colony shape, the result is as follows figure 1 Shown; Gram staining of Bacillus coagulans BC99, followed by microscopic observation, the results are as follows figure 2 shown.

[0031] From figure 1 and figure 2 It can be seen from the figure that the colony grown on the surface of the beef extract peptone medium is off-white, with a diameter of 0.5-4mm, round shape, neat edges, slightly raised, and shiny surface. Under the microscope, it appears as a Gram-positive long rod with terminal spores.

[0032] The preparation method of beef extract peptone medium is as follows: every 1000mL beef extract peptone medium contains 5g beef extract, 10g peptone, 5g sodium chloride, 1000mL distilled water, 15g agar, pH7.0-7.2, sterilized at 118°C for 15min.

Embodiment 3

[0034] Preparation of Bacillus coagulans BC99 bacterial liquid

[0035] Bacteria solution without spore formation: inoculate Bacillus coagulans BC99 stored at -80°C in liquid MRS medium, culture in a shaker incubator at 42°C, 180r / min-250r / min, for 12h-16h, to obtain bacteria solution, marked as bacterial solution 1.

[0036] Spore-forming spores: Inoculate Bacillus coagulans BC99 stored at -80°C in liquid MRS medium, culture in a shaker incubator at 42°C, 180r / min-250r / min, and cultivate for 24h-36h to obtain bacterial liquid, label For bacterial solution 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bacillus coagulans BC99 and a screening method thereof. The preservation number of the bacillus coagulans is CGMCC No.19487, the preservation time is March 18, 2020, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The bacillus coagulans BC99provided by the invention is derived from baby excrement, has relatively strong stress resistance, has relatively high tolerance to high temperature, artificial gastric juice, artificial bile salt and artificial intestinal juice, can preserve relatively high survival rate, and is suitable for being applied to food processing and animal husbandry.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus coagulans BC99 and a screening method thereof. Background technique [0002] Lactic acid bacteria (LAB) are abundant in the intestinal tract and oral cavity of animals. Lactic acid bacteria can use carbohydrates to produce organic acids, special enzymes, lactobacillus, etc. Lactic acid bacteria can promote animal growth, regulate normal gastrointestinal flora and maintain micro-ecological balance, thereby improving gastrointestinal function, increasing food digestibility and biological potency, controlling endotoxin, inhibiting the growth of spoilage bacteria in the intestinal tract, and improving the body's immunity etc., widely used in food industry and animal husbandry industry. [0003] Currently widely used probiotics are mainly Bifidobacterium, Lactobacillus acidophilus, Enterococcus faecalis and Enterococcus faecium, etc., but the above-mentioned probio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 方曙光杜玮瑶朱明明
Owner 武汉微康益生菌研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com