Precise preparation of acetic acid standard curve solution and detection method of protected amino acid acetic acid

Abstract

The invention discloses a method for accurately preparing an acetic acid standard curve solution and protecting amino acid acetic acid, comprising the following steps: S1, preparing an acetic acid stock solution, accurately weighing a designed amount of anhydrous potassium acetate, and adding ultrapure water according to the detection concentration requirement After constant volume, obtain the acetic acid stock solution of design concentration; S2, prepare the acetic acid standard curve solution, add ultrapure water to dilute the stock solution, obtain the acetic acid standard curve solution of design concentration, when preparing, add trifluoroethanol or methyl alcohol of design amount and other steps. The present invention makes the preparation process of the standard curve more convenient and accurate by improving the formula of the standard curve, avoids the use of organic solvents, not only reduces the volatilization of the solvent, but also reduces the detection cost; at the same time, it detects by using ion chromatography, and through the design Reasonable gradient conditions can effectively separate the interference peak from the target peak, and finally make the target peak appear obvious, easy to judge and calculate, and solve the problem of misjudgment and misjudgment in the prior art.

Description

technical field [0001] The invention relates to the technical field of acetic acid detection of protected amino acids, in particular to a method for accurately preparing a standard curve solution of acetic acid and a detection method of acetic acid of protected amino acids. Background technique [0002] With the development of peptide synthesis, the market demand for protected amino acids is increasing, and the quality requirements for protected amino acids are becoming more and more stringent. Through a lot of practice, the acetic acid impurity contained in the protected amino acid has a great negative impact on the quality control of the final product, so the customer is very strict in the control of the acetic acid in the protected amino acid, usually controlled below 200ppm, and even less than 50ppm , and how to precisely control the acetic acid content, first of all, an efficient detection method with high accuracy and high detection precision is needed to detect the ac...

AI Technical Summary

Problems solved by technology

[0007] The first object of the invention of the present invention is: when preparing the acetic acid standard curve solution in the prior art, the concentration that exists is unstable, inaccurate, and the problem that the standard curve error is large provides a kind of method for accurately preparing the acetic acid standard curve solution, To solve the problems existing in the existing technology

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