Deep infrared ratio type fluorescent probe capable of quickly responding to sulfur dioxide

A fluorescent probe and fast-response technology, applied in the field of fluorescent probes, can solve problems such as expensive instruments, complicated operation steps, and long response time, and achieve high selectivity and strong fluorescence emission effects

Active Publication Date: 2020-08-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have disadvantages such as complex operation steps, expensive instruments, long response time, etc.

Method used

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  • Deep infrared ratio type fluorescent probe capable of quickly responding to sulfur dioxide
  • Deep infrared ratio type fluorescent probe capable of quickly responding to sulfur dioxide
  • Deep infrared ratio type fluorescent probe capable of quickly responding to sulfur dioxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Synthesis of probe

[0025] 0.2 g of compound Cou-Acetyl and 0.18 g of 4-diethylaminosalicylic aldehyde were dissolved in 2.9 ml of concentrated sulfuric acid. The above system was stirred and refluxed at 90 degrees Celsius for 8 hours. After the reaction, it was cooled to room temperature, and 2.9 ml of high chlorine was added dropwise. Acid, add 50 ml of ice water, filter with suction and wash with ice water, and purify the obtained suction filtrate by column chromatography to obtain blue-purple solid probe 1, with a yield of 80%. figure 1 Is the NMR image of the probe.

Embodiment 2

[0026] Example 2 Selective analysis of probes

[0027] A mixture of EtOH and HEPES with a volume ratio of 1:9 containing 10 μmol / L probe 1 was added to a sodium bisulfite solution with a concentration of 200 μmol / L at one time to detect the change in fluorescence of the solution. The result is figure 2 , When the excitation light wavelength is 470nm, the probe is very sensitive to NaHSO 3 There is a strong orange fluorescence at 610nm, and the probe has no obvious response to other anions, cations, and sulfhydryl amino acids. It shows that the probe has excellent selectivity to sulfur dioxide.

Embodiment 3

[0028] Example 3 Analysis of probe response to changes in sulfur dioxide concentration

[0029] Add different concentrations of sodium bisulfite solution to a 1:9 mixture of EtOH and HEPES containing 10 μmol / L of probe 1, and use 470nm and 654nm for excitation. Probe 1 is at 610nm and 690nm. The ratio of the fluorescence intensity at the position changes regularly with the increase of the amount of sodium bisulfite. Such as image 3 with 4 , Indicating that the probe can respond sensitively to changes in the concentration of sodium bisulfite under the excitation of two wavelengths of light.

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PUM

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Abstract

The invention discloses a fluorescent probe for detecting sulfur dioxide, and belongs to the technical field of analytical chemistry. The probe is prepared from prepared novel coumarin dye and 4-diethylamino salicylaldehyde in concentrated sulfuric acid at 90 DEG C. The preparation raw materials of the fluorescent probe are easy to obtain, the synthesis method is simple, and the fluorescent probehas the advantages of high selectivity, high sensitivity, low detection limit and the like for sulfur dioxide. The fluorescent probe can be applied to detection of sulfur dioxide in cells.

Description

Technical field [0001] The invention provides a fluorescent probe for detecting sulfur dioxide, which belongs to the technical field of fluorescent probes. [0002] technical background [0003] Sulfur Dioxide (SO 2 ) Is an important chemical raw material that is widely used in various industries, such as pulp manufacturing, metal processing and food preservatives. At the same time, it is also one of the main pollutants of the environment. 3 - It exists in the form of sulfur and can be produced by oxidation of sulfur-containing amino acids or decomposition of sulfapyruvate. More and more studies have shown that SO 2 As an antioxidant, it plays a positive role in fighting ROS and regulating the redox state. At the same time, sulfur dioxide is also an important endogenous gas transmitter, which plays a vital role in many physiological activities, such as vasodilation, muscle relaxation, and signal transduction. Abnormal intracellular levels are closely related to certain diseases, ...

Claims

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Application Information

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IPC IPC(8): C07D311/92C09K11/06G01N21/64
CPCC07D311/92C09K11/06G01N21/6428C09K2211/1088G01N2021/6439
Inventor 吕正亮孙鑫蒋绪川范春华
Owner UNIV OF JINAN
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