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Application of PGAM5 as diagnostic marker and therapeutic target for oligospermia and asthenozoospermia

A technology for asthenozoospermia and oligospermia, applied in the field of oligospermia diagnostic markers, can solve the problems of little understanding, and achieve the effect of high accuracy and diagnostic value

Pending Publication Date: 2020-08-11
山东立菲生物产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] PGAM5 (The protein phosphoglycerate mutase 5) is a serine / threonine protein phosphatase, the activation of PGAM5 can lead to linear breakage of mitochondria arranged in series, which is related to mitochondrial apoptosis, necrosis and autophagy, and PGAM5 in Occupying a pivotal role in the cell necrosis pathway caused by a variety of reasons, involved in cell necrosis caused by excessive growth of oxygen free radicals and excessive leakage of calcium ions, etc., but the mechanism of cell necrosis caused by PGAM5 is currently poorly understood

Method used

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  • Application of PGAM5 as diagnostic marker and therapeutic target for oligospermia and asthenozoospermia
  • Application of PGAM5 as diagnostic marker and therapeutic target for oligospermia and asthenozoospermia
  • Application of PGAM5 as diagnostic marker and therapeutic target for oligospermia and asthenozoospermia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Detection method

[0028] In this example, a method for detecting oligospermia markers based on sperm proteomics is provided. The samples used in the following examples are all from the Reproductive Hospital Affiliated to Shandong University. The forward movement of sperm in samples from patients with oligoasthenospermia is 7.8 %-17.3, the sperm concentration is between 3.8-14.8 million / mL, the detection method includes the following steps:

[0029] (1) Extract the total protein of sperm cells: wash the sperm sample with DPBS, add RIPA lysis buffer and ultrasonic for 1 to 2 minutes, incubate on ice for 30 minutes to lyse, centrifuge at 14,000g for 20 minutes, take the supernatant protein liquid, use the Bradford method to determine the liquid Medium protein concentration.

[0030] (2) Enzymatically digest the protein using the method based on ultrafiltration assisted sample preparation (FASP): add 300μg protein solution to a 10KD ultrafiltration tube, shake at 600RPM for 1...

Embodiment 2

[0049] Example 2 Clinical test verification

[0050] According to the method of Example 1, the collected sperm of 10 groups of oligoasthenospermia patients were firstly subjected to protein extraction and nanoflow liquid chromatography-tandem mass spectrometry analysis, and each set of data was repeated three times, and then the mass spectrometry data was analyzed. Table 1 shows the results of the analysis. 10 people were detected with oligoasthenospermia using the above method. The accuracy of this set of data is as high as 100%.

[0051] Table 1

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[0064]

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Abstract

The invention belongs to the technical field of diagnosis for oligospermia and asthenozoospermia, and particularly relates to application of PGAM5 as a diagnostic marker and a therapeutic target for oligospermia and asthenozoospermia. On the basis of a proteomics method, protein data of normal patient samples and patient samples with oligospermia and asthenozoospermia are analyzed, BSA protein signal strength is taken as the standard to detect protein abundance of the samples, and differential protein of the normal patient samples and the diseased patient samples is obtained. By means of detection with the method, remarkable down-regulation of the PGAM5 in the samples with severe oligospermia and asthenozoospermia is determined, the PGAM5 has good credibility when serving as the diagnosticbiomarker, and the PGAM5 is applied to development of related detection reagents and kits. Meanwhile, the research result is conducive to clarifying the pathogenesis of oligospermia and asthenozoospermia, contributes to development of target drugs for oligospermia and asthenozoospermia and has great clinical significance.

Description

Technical field [0001] The present invention belongs to the field of diagnostic markers for oligospermia, and specifically relates to PGAM5 as a diagnostic marker or treatment target for oligospermia, weak sperm and even severe oligospermia. It is used in the preparation of diagnostic reagents or the treatment of oligospermia, weak sperm and severe oligospermia. The application of disease drugs. Background technique [0002] Disclosure of the background information is only intended to increase the understanding of the overall background of the present invention, and is not necessarily regarded as an acknowledgement or in any form suggesting that the information constitutes the prior art known to those of ordinary skill in the art. [0003] According to a survey by the World Health Organization, 15% of couples of childbearing age have infertility problems. Infertility has become a global medical and social problem affecting human health and social development (Turner, TT and JJ Lysi...

Claims

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Application Information

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IPC IPC(8): C12Q1/42A61K45/00A61P15/08
CPCA61K45/00A61P15/08C12Q1/42G01N2333/916G01N2800/367
Inventor 黄宁董锡阳张亚卓张曼玉孙胜楠
Owner 山东立菲生物产业有限公司
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