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Autologous cord blood treatment method for preventing complications of premature infants and application of autologous cord blood treatment method

A processing method, the technology of umbilical cord blood, applied in the field of umbilical cord blood processing, can solve the problems of reduced repair ability, developmental disorder of organ function, lack of precursor cells of multiple organs, etc., to achieve reduced toxicity, safe method, shortening of mechanical ventilation and oxygen supplementation the effect of time

Inactive Publication Date: 2020-08-11
广州市天河诺亚生物工程有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

The existing treatment methods for complications of premature infants are single-organ symptomatic treatment, which fails to solve the fundamental problems of lack of precursor cells in multiple organs, reduced repair ability after injury, and organ dysfunction.

Method used

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  • Autologous cord blood treatment method for preventing complications of premature infants and application of autologous cord blood treatment method
  • Autologous cord blood treatment method for preventing complications of premature infants and application of autologous cord blood treatment method
  • Autologous cord blood treatment method for preventing complications of premature infants and application of autologous cord blood treatment method

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Embodiment 1

[0048] Example 1: A method for processing autologous cord blood that can be used to prevent complications in premature infants

[0049] A method for processing autologous umbilical cord blood that can be used to prevent complications in premature infants, comprising the following steps:

[0050] (1) Autologous umbilical cord blood is stored and transported to the umbilical cord blood hematopoietic stem cell bank

[0051] Autologous umbilical cord blood (from Guangdong Province) was collected in the operating room and shaken well, and immediately stored in a refrigerator at 0-4°C, until the staff collected it and put it in a blood transport box at 4-15°C for transport to cord blood hematopoietic stem cells library. The information of each umbilical cord blood is reviewed by two people. After heat sealing to remove the blood collection needle and redundant tubing, the blood bag is weighed and the amount of umbilical cord blood is calculated. Each unit of cord blood is encoded ...

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Abstract

The invention provides an autologous cord blood treatment method for preventing complications of premature infants and an application of the autologous cord blood treatment method, and belongs to thetechnical field of cord blood treatment. The autologous cord blood treatment method for preventing complications of premature infants comprises the following steps of (1) collecting autologous cord blood, and storing and transporting the autologous cord blood to a cord blood hemopoietic stem cell storehouse; (2) counting nucleated cells of the cord blood, and counting the number of total nucleatedcells; (3) performing virus detection on the cord blood; and (4) in a sterile operation room, performing inversed centrifugation on a blood bag, removing red blood cells, then performing positive centrifugation on the blood bag, and performing separating to obtain plasma, so that the autologous cord blood nucleated cells are obtained. Besides, nucleated cell activity and CD34+ cellularity of theprepared nucleated cells are analyzed. The method is safe, time-saving, and high in repeatability, and conforms to clinical requirements. A safe and reliable autologous cord blood treatment techniquefor preventing complications of premature infants is provided for clinical application.

Description

technical field [0001] The invention belongs to the technical field of umbilical cord blood processing, and in particular relates to an autologous umbilical cord blood processing method and application thereof which can be used to prevent complications of premature infants. Background technique [0002] For a long time, umbilical cord blood was considered as biological waste and was directly discarded. With the continuous progress of scientific research, people gradually realized the importance of umbilical cord blood. In 1988, France carried out the world's first umbilical cord blood transplantation to treat Fanconi anemia, and people began to realize the medical value of umbilical cord blood, and umbilical cord blood gradually developed into the third largest source of hematopoietic stem cells after bone marrow and peripheral blood . Umbilical cord blood is rich in various stem cells and various cytokines, and has been widely used in clinical trials of regenerative medici...

Claims

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Application Information

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IPC IPC(8): A61M1/02G01N33/569C12Q1/06
CPCA61M1/0259A61M1/0272G01N33/49G01N33/56983
Inventor 嵐山芮魏伟史聪颖
Owner 广州市天河诺亚生物工程有限公司
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