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Method for simultaneously determining four effective components in Sihuangzhili Granules

A technology of Sihuang Zhili granules and active ingredients, which is applied in the directions of measuring devices, material separation, and analysis materials, etc., can solve the problem that the quality of Sihuang Zhili granules is difficult to be fully controlled, and achieve cheap and easy-to-obtain reagents and consumables, exclusive Strong, high-precision effect

Active Publication Date: 2020-08-07
CHONGQING FANGTONG ANIMAL PHARMA
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Problems solved by technology

The 2015 edition of "Chinese Veterinary Pharmacopoeia" requires only the determination of the content of baicalin, as well as the TLC identification of berberine hydrochloride and rhubarb as reference medicinal materials. There are no detection requirements for other ingredients. In this way, the effective substances will degrade after high temperature Obviously, it is difficult to fully control the quality of Sihuang Zhili Granules
[0006] However, the simultaneous determination of several components is rarely reported

Method used

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  • Method for simultaneously determining four effective components in Sihuangzhili Granules
  • Method for simultaneously determining four effective components in Sihuangzhili Granules
  • Method for simultaneously determining four effective components in Sihuangzhili Granules

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Embodiment 1

[0049] In this embodiment, the method for simultaneously determining four active ingredients in Sihuang Zhili Granules is the HPLC-DAD method, which comprises the following steps:

[0050] 1) Chromatographic conditions

[0051] Column: SunFire TM C18 (4.6mm×150mm, 5μm);

[0052] Chromatographic column filler: octadecylsilane bonded silica gel;

[0053]Mobile phase and elution method: 0.1% phosphoric acid aqueous solution is used as mobile phase A, acetonitrile is used as mobile phase B, and gradient elution is carried out according to the following procedure,

[0054] 0~5min: The mobile phase is a mobile phase A-mobile phase B mixture with a volume ratio of 92:8;

[0055] 5 to 10 minutes: The mobile phase is a mobile phase A-mobile phase B mixture with a volume ratio of 85:15;

[0056] 10-20min: The mobile phase is a mobile phase A-mobile phase B mixture with a volume ratio of 80:20;

[0057] 20-30min: The mobile phase is a mobile phase A-mobile phase B mixture with a vol...

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Abstract

The invention provides a method for simultaneously detecting four effective components in Sihuangzhili Granules, and belongs to the technical field of veterinary drug detection. According to the method, original standards and effective components of the Sihuangzhili Granules are taken as the basis; four effective components in the Sihuangzhili Granules are accurately analyzed and detected; the quality of the Sihuangzhili Granules can be further effectively controlled; the preparation quality is comprehensively evaluated; therefore, the stability of product quality and the safety and effectiveness of veterinary drugs are ensured to the greatest extent; the method has the advantages of strong specificity, high precision, good stability, high accuracy, simple operation, reagents and consumables cheap and easy to obtain, effective control of the production quality and the stability of the product, improvement of the quality and the market competitiveness of the product, and promotion and practical values in the pharmaceutical industry.

Description

technical field [0001] The invention relates to the technical field of veterinary drug detection, in particular to a method for simultaneously determining four active ingredients in Sihuang Zhili Granules. Background technique [0002] Sihuang Zhili Granules are made of Chinese medicines of Coptis chinensis, Cortex Phellodendron, Rhubarb, Scutellaria Radix, Banlangen, and Licorice, which are decocted twice in water, concentrated, granulated and dried with auxiliary materials. The disease has a good therapeutic effect, and it is included in the second part of the 2015 edition of "Chinese Veterinary Pharmacopoeia". [0003] There are also many patents on Sihuang Zhili Granules in China. For example, Chinese patent 200910066083.3 discloses a preparation method of Sihuang Zhili Effervescent Granules. The Sihuang Zhili Effervescent Granules are composed of the following raw materials and auxiliary materials Prepared: Raw materials: Coptidis 200, Phellodendron 200, Rhubarb 100, S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/74
CPCG01N30/88G01N30/74G01N2030/8809
Inventor 毕海林肖裕章张超袁启峰
Owner CHONGQING FANGTONG ANIMAL PHARMA
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