Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method of oak bark volatile oil as well as detection method and application of oak bark volatile oil

A technology of extraction method and detection method, which is applied in the field of extraction of volatile oil from oak bark, can solve problems such as bleeding, large side effects, and accelerated cardiovascular diseases, and achieve high anticoagulant effect

Active Publication Date: 2020-07-31
JILIN INST OF CHEM TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

About 290 million patients in my country suffer from cardiovascular diseases, and the mortality rate of cardiovascular diseases is higher than that of malignant tumors, accounting for more than 40% of disease-related deaths; coagulation can lead to the formation of thrombus, thereby accelerating the progress of cardiovascular diseases; Therefore, anticoagulation is the basis and key to prevent and treat cardiovascular diseases; at present, anticoagulant drugs have been widely used in the treatment of disseminated intravascular coagulation and thrombosis in various diseases, and natural antioxidants also play a role in thrombosis. It plays a pivotal role; since the 1940s, people have used heparin as the main drug for the treatment and prevention of venous thrombosis and thromboembolism. However, long-term use of heparin has the risk of bleeding and has relatively large side effects; therefore, research and development have small side effects and Effective Heparin Substitute Has Practical Significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction method of oak bark volatile oil as well as detection method and application of oak bark volatile oil
  • Extraction method of oak bark volatile oil as well as detection method and application of oak bark volatile oil
  • Extraction method of oak bark volatile oil as well as detection method and application of oak bark volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of extraction method of eucalyptus bark volatile oil, the steps are as follows:

[0034] Take 100g of eucalyptus bark and crush it, add distilled water, stir, soak, carry out steam distillation extraction with a volatile oil extraction device, select the optimal extraction conditions: the ratio of material to liquid is 1:5 (g / mL), and the extraction time is 5h to obtain eucalyptus bark The extraction rate of volatile oil is 0.08%±0.03%, and the extraction rate is 30% higher than other solid-liquid ratios (1:4~7) and extraction time (4~6h). The oil and water are separated to obtain a sample, and before gas chromatography-mass spectrometry (GC-MS) analysis, the volatile oil is stored in a sealed bottle and placed in the freezing layer of the refrigerator.

Embodiment 2

[0036] A method for identifying and analyzing the eucalyptus bark volatile oil prepared by the above-mentioned extraction method, the steps are as follows:

[0037]The detection of volatile oil includes: the volatile oil of the species to be tested is analyzed and detected by gas chromatography-mass spectrometry (GC-MS). The flow rate is that the initial temperature is 60°C, keep it for 6 minutes, raise the temperature to 300°C at 3°C / min, and keep it for 10 minutes. The ion source temperature is 230°C, the electron bombardment ion source (EI) energy is 70eV, the transfer line temperature is 280°C, the scanning range (m / z): 40-450, and the mass spectrum search library: Nist05.

[0038] By comparison with the standard alkane series (C 8 -C 40 ) and compare its mass spectrum with the reference data in the equipment database (NIST 05) and literature to determine the characteristics of each component. The results are shown in Table 1.

[0039] Table 1 GC-MS analysis results of ...

Embodiment 3

[0063] Adopt Fourier Transform Infrared Spectroscopy to proofread the main components detected by gas chromatography-mass spectrometry, and detect the chemical composition of the eucalyptus bark volatile oil and its main component (pentadecanoic acid), the steps are as follows:

[0064] Mix n-pentadecanoic acid and potassium bromide, grind them at a ratio of 1:100, press them into tablets, apply the volatile oil of oak bark evenly on the KBr solid window, scan the blank spectrum respectively, and use FT-IR spectrophotometry Measured at 4000cm -1 to 400cm -1 The detection was performed 20 times in between, and repeated every 3 minutes.

[0065] As attached to the manual figure 1 The infrared spectrum shown shows that the functional group absorption peak characteristic peaks of oak bark volatile oil and n-pentadecanoic acid are partially similar, and their infrared spectra are mostly consistent, and the GC-MS detection is scientific and reasonable.

[0066] In the attached f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant active ingredient extraction, and particularly relates to an extraction method, a detection method and application of oak bark volatile oil. A steam distillation method is adopted for extraction; the chemical components, antioxidant activity and anticoagulant activity of the oak bark volatile oil are explored, and GC-MS and FT-IR are adopted for analyzing the chemical components of the oak bark volatile oil. 28 components are identified in the oak bark volatile oil and account for 96.98% of the total mass of the oak bark volatile oil. A functional group deduced from an FT-IR curve fitting result is verified through GC-MS analysis, and the FT-IR spectrum of the oak bark volatile oil is consistent with the FT-IR spectrum of an n-pentadecanoic acid functional group. Based on the outstanding antioxidant and anticoagulant activities of the oak bark volatile oil, a soft capsule dosage form with antioxidant and anticoagulant effects is developed, and a new economic value is provided for comprehensive development and utilization of plant resources.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting volatile oil from oak bark, a detection method and application thereof. Background technique [0002] Oak tree, also known as Quercus mongolica Fagaceae and Quercus genus, is mainly distributed in Japan, South Korea, the Russian Far East, the Korean Peninsula, and northern and northeast China. There are about 600 species; oak tree, as a temperate deciduous tree, has important ecological and economic value; which contains sterols, triterpenes, glycosides, flavonoids, phenols, coumarins, tannic acid and other chemical substances. [0003] The wood of oak trees can be used to make furniture, the fruit can be used to produce starch, and the leaves are used to breed tussah silkworms, which have high nutritional value. In northern China, the supply exceeds demand, so the planting area of ​​oak trees is increasing year by year, and the number of discarde...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A61K36/49A61K9/48A61P7/02A61P39/06G01N21/3577G01N30/02A61K129/00
CPCC11B9/027A61K36/49A61P7/02A61P39/06A61K9/4825G01N30/02G01N21/3577G01N2021/3595A61K2236/37Y02A50/30
Inventor 周鸿立田海苹翟婉辰孙昆朱蕴文
Owner JILIN INST OF CHEM TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com