Method for identifying production place of pericarpium citri reticulatae based on autofluorescence-quenched fluorescence spliced spectrum
An autofluorescence and fluorescence spectrum technology, which is applied in the field of identifying the origin of tangerine peel based on autofluorescence-quenching fluorescence splicing spectrum, can solve the problems of requiring large analytical instruments, consumption, and many harmful organic reagents, and achieves high sensitivity, strong specificity, The effect of improving the recognition rate
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[0049] A method for identifying the origin of tangerine peel based on autofluorescence-quenched fluorescence mosaic spectrum, comprising the steps of:
[0050] 1) Synthesis of CdTe quantum dots:
[0051] Add 0.0457g of cadmium dichloride and 18 μL of mercaptoacetic acid into 50mL of ultrapure water, stir at normal temperature and pressure to fully dissolve it; then add 1.0mol / L of sodium hydroxide solution to adjust the pH value of the resulting solution to 10.3, and then Introduce nitrogen and stir for 20 minutes under ice bath conditions; add 0.0089g sodium tellurite, stir for 15 minutes, then add 0.08g sodium borohydride, continue stirring for 10min; transfer the resulting clear solution to an oil bath, 100 ℃ oil bath refluxed for 1.5h, and the emission wavelength was about 550nm, and the orange-yellow CdTe quantum dot fluorescent probe (its fluorescence spectrum as shown in Fig. figure 2 shown);
[0052] 2) Preparation of dried tangerine peel aqueous extract:
[0053] ...
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